Skip to main content

Differential production of high molecular weight sulfated glycoproteins in normal colonic mucosa, primary colon carcinoma, and metastases.

Publication ,  Journal Article
Yamori, T; Kimura, H; Stewart, K; Ota, DM; Cleary, KR; Irimura, T
Published in: Cancer Res
May 15, 1987

Sulfated macromolecules synthesized in tumor and mucosa tissues derived from colorectal cancer patients were labeled with [35S]sulfate and separated into two fractions on DEAE-Sephacel: the slightly acidic peak (peak I) was eluted with 0.2 M NaCl and the highly acidic peak (peak II) was eluted with 0.5 M NaCl. A total of 40 specimens, which included primary colon cancer, liver metastases, and normal mucosa obtained at surgery (16 patients), were examined regarding the amount of peak I and peak II. The amount of peak I significantly decreased in the order of normal mucosa greater than primary tumors greater than metastases, while the amount of peak II did not significantly change among the tissues. Peak I was mostly resistant to chondroitinase ABC and nitrous acid treatment under acidic conditions, whereas combined chondroitinase-sensitive materials and nitrous acid-sensitive materials were greater than 80% of the radioactivity in peak II. The major radioactive component of peak I migrated at a position corresponding to Mr greater than 300,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and became Mr less than 40,000 after alkaline borohydride treatment. The major component of peak I was likely to be a sulfated glycoprotein containing sulfate groups on alkaline labile carbohydrate chains. Peak II consisted of a mixture of heparan sulfate proteoglycans and chondroitin sulfate proteoglycans. Differential incorporation of [35S]sulfate into peak I among normal mucosa, primary colon carcinoma, and colon carcinoma metastasis was observed. Therefore, decreased peak I production may be a biochemical change associated with colorectal cancer progression and metastasis.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

May 15, 1987

Volume

47

Issue

10

Start / End Page

2741 / 2747

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Mucins
  • Molecular Weight
  • Molecular Chaperones
  • Intestinal Mucosa
  • Humans
  • Glycoproteins
  • Electrophoresis, Polyacrylamide Gel
  • Colonic Neoplasms
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yamori, T., Kimura, H., Stewart, K., Ota, D. M., Cleary, K. R., & Irimura, T. (1987). Differential production of high molecular weight sulfated glycoproteins in normal colonic mucosa, primary colon carcinoma, and metastases. Cancer Res, 47(10), 2741–2747.
Yamori, T., H. Kimura, K. Stewart, D. M. Ota, K. R. Cleary, and T. Irimura. “Differential production of high molecular weight sulfated glycoproteins in normal colonic mucosa, primary colon carcinoma, and metastases.Cancer Res 47, no. 10 (May 15, 1987): 2741–47.
Yamori T, Kimura H, Stewart K, Ota DM, Cleary KR, Irimura T. Differential production of high molecular weight sulfated glycoproteins in normal colonic mucosa, primary colon carcinoma, and metastases. Cancer Res. 1987 May 15;47(10):2741–7.
Yamori T, Kimura H, Stewart K, Ota DM, Cleary KR, Irimura T. Differential production of high molecular weight sulfated glycoproteins in normal colonic mucosa, primary colon carcinoma, and metastases. Cancer Res. 1987 May 15;47(10):2741–2747.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

May 15, 1987

Volume

47

Issue

10

Start / End Page

2741 / 2747

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Mucins
  • Molecular Weight
  • Molecular Chaperones
  • Intestinal Mucosa
  • Humans
  • Glycoproteins
  • Electrophoresis, Polyacrylamide Gel
  • Colonic Neoplasms