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Expression of Neurogenin 1 in mouse embryonic stem cells directs the differentiation of neuronal precursors and identifies unique patterns of down-stream gene expression.

Publication ,  Journal Article
Velkey, JM; O'Shea, KS
Published in: Dev Dyn
March 2013

BACKGROUND: Delineating the cascades of growth and transcription factor expression that shape the developing nervous system will improve our understanding of its molecular histogenesis and suggest strategies for cell replacement therapies. In the current investigation, we examined the ability of the proneural gene, Neurogenin1 (Neurog1; also Ngn1, Neurod3), to drive differentiation of pluripotent embryonic stem cells (ESC). RESULTS: Transient expression of Neurog1 in ESC was sufficient to initiate neuronal differentiation, and produced neuronal subtypes reflecting its expression pattern in vivo. To begin to address the molecular mechanisms involved, we used microarray analysis to identify potential down-stream targets of Neurog1 expressed at sequential stages of neuronal differentiation. CONCLUSIONS: ESC expressing Neurogenin1 begin to withdraw from cycle and form precursors that differentiate exclusively into neurons. This work identifies unique patterns of gene expression following expression of Neurog1, including genes and signaling pathways involved in process outgrowth and cell migration, regional differentiation of the nervous system, and cell cycle.

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Published In

Dev Dyn

DOI

EISSN

1097-0177

Publication Date

March 2013

Volume

242

Issue

3

Start / End Page

230 / 253

Location

United States

Related Subject Headings

  • Signal Transduction
  • Oligonucleotide Array Sequence Analysis
  • Neurons
  • Neural Stem Cells
  • Nerve Tissue Proteins
  • Mice
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Embryonic Stem Cells
  • Developmental Biology
 
Journal cover image

Published In

Dev Dyn

DOI

EISSN

1097-0177

Publication Date

March 2013

Volume

242

Issue

3

Start / End Page

230 / 253

Location

United States

Related Subject Headings

  • Signal Transduction
  • Oligonucleotide Array Sequence Analysis
  • Neurons
  • Neural Stem Cells
  • Nerve Tissue Proteins
  • Mice
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Embryonic Stem Cells
  • Developmental Biology