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Pax7 expressing cells contribute to dermal wound repair, regulating scar size through a β-catenin mediated process.

Publication ,  Journal Article
Amini-Nik, S; Glancy, D; Boimer, C; Whetstone, H; Keller, C; Alman, BA
Published in: Stem Cells
September 2011

During skin wound healing, fibroblast-like cells reconstitute the dermal compartment of the repaired skin filling the wound gap. A subset of these cells are transcriptionally active for β-catenin/T-cell factor (TCF) signaling during the proliferative phase of the repair process, and β-catenin levels control the size of the scar that ultimately forms by regulating the number of dermal fibroblasts. Here, we performed cell lineage studies to reveal a source of the dermal cells in which β-catenin signaling is activated during wound repair. Using a reporter mouse, we found that cells in the early wound in which TCF-dependent transcription is activated express genes involved in muscle development. Using mice in which cells express Pax7 (muscle progenitors) or Mck (differentiated myocytes) are permanently labeled, we showed that one quarter of dermal cells in the healing wound are Pax7 expressing progeny, but none are Mck progeny. Removing one allele of β-catenin in Pax7 expressing progeny resulted in a significantly smaller scar size with fewer Pax7 expressing progeny cell contributing to wound repair. During wound healing, β-catenin activation causes muscle satellite cells to adopt a fibrotic phenotype and this is a source of dermal cells in the repair process.

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Published In

Stem Cells

DOI

EISSN

1549-4918

Publication Date

September 2011

Volume

29

Issue

9

Start / End Page

1371 / 1379

Location

England

Related Subject Headings

  • beta Catenin
  • Wound Healing
  • TCF Transcription Factors
  • Skin
  • PAX7 Transcription Factor
  • Mice, Transgenic
  • Mice
  • Immunology
  • Immunohistochemistry
  • Gene Expression
 

Citation

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Amini-Nik, S., Glancy, D., Boimer, C., Whetstone, H., Keller, C., & Alman, B. A. (2011). Pax7 expressing cells contribute to dermal wound repair, regulating scar size through a β-catenin mediated process. Stem Cells, 29(9), 1371–1379. https://doi.org/10.1002/stem.688
Amini-Nik, Saeid, Dylan Glancy, Corey Boimer, Heather Whetstone, Charles Keller, and Benjamin A. Alman. “Pax7 expressing cells contribute to dermal wound repair, regulating scar size through a β-catenin mediated process.Stem Cells 29, no. 9 (September 2011): 1371–79. https://doi.org/10.1002/stem.688.
Amini-Nik S, Glancy D, Boimer C, Whetstone H, Keller C, Alman BA. Pax7 expressing cells contribute to dermal wound repair, regulating scar size through a β-catenin mediated process. Stem Cells. 2011 Sep;29(9):1371–9.
Amini-Nik, Saeid, et al. “Pax7 expressing cells contribute to dermal wound repair, regulating scar size through a β-catenin mediated process.Stem Cells, vol. 29, no. 9, Sept. 2011, pp. 1371–79. Pubmed, doi:10.1002/stem.688.
Amini-Nik S, Glancy D, Boimer C, Whetstone H, Keller C, Alman BA. Pax7 expressing cells contribute to dermal wound repair, regulating scar size through a β-catenin mediated process. Stem Cells. 2011 Sep;29(9):1371–1379.
Journal cover image

Published In

Stem Cells

DOI

EISSN

1549-4918

Publication Date

September 2011

Volume

29

Issue

9

Start / End Page

1371 / 1379

Location

England

Related Subject Headings

  • beta Catenin
  • Wound Healing
  • TCF Transcription Factors
  • Skin
  • PAX7 Transcription Factor
  • Mice, Transgenic
  • Mice
  • Immunology
  • Immunohistochemistry
  • Gene Expression