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PTHrP regulates growth plate chondrocyte differentiation and proliferation in a Gli3 dependent manner utilizing hedgehog ligand dependent and independent mechanisms.

Publication ,  Journal Article
Mau, E; Whetstone, H; Yu, C; Hopyan, S; Wunder, JS; Alman, BA
Published in: Dev Biol
May 1, 2007

Growth plate chondrocytes undergo a tightly regulated process of differentiation, allowing for the longitudinal growth of bones. Although it is known that parathyroid hormone related protein (PTHrP) and Indian hedgehog regulate the differentiation of growth plate chondrocytes, how these pathways interact to regulate chondrocyte development is not fully elucidated. We examined how the interaction between PTHrP and the hedgehog activated transcription factors, Gli2 and Gli3, regulates growth plate chondrocyte differentiation and proliferation. Analysis of fetal limbs showed that Gli2 is a negative regulator and Gli3 a positive regulator of type X collagen expression. Limb explant cultures showed that PTHrP treatment inhibited type X collagen expression and increased chondrocyte proliferation. This effect was substantially enhanced in Gli2-/- limbs, was blocked in Gli3-/- limbs, and was only partially inhibited by hedgehog ligand blockade. PTHrP negatively regulated Gli mediated transcription in cell cultures, and regulated the level of the repressor form of Gli3 in a PKA dependent manner. These results show that PTHrP regulates growth plate chondrocyte proliferation and differentiation in part through the activity of Gli3, suggesting a crucial role for Gli3 in growth plate chondrocyte development.

Duke Scholars

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

May 1, 2007

Volume

305

Issue

1

Start / End Page

28 / 39

Location

United States

Related Subject Headings

  • Zinc Finger Protein Gli3
  • Zinc Finger Protein Gli2
  • Signal Transduction
  • Protein Serine-Threonine Kinases
  • Parathyroid Hormone-Related Protein
  • Nerve Tissue Proteins
  • Mice, Knockout
  • Mice
  • Kruppel-Like Transcription Factors
  • In Situ Nick-End Labeling
 

Citation

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Chicago
ICMJE
MLA
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Mau, E., Whetstone, H., Yu, C., Hopyan, S., Wunder, J. S., & Alman, B. A. (2007). PTHrP regulates growth plate chondrocyte differentiation and proliferation in a Gli3 dependent manner utilizing hedgehog ligand dependent and independent mechanisms. Dev Biol, 305(1), 28–39. https://doi.org/10.1016/j.ydbio.2007.01.031
Mau, Elaine, Heather Whetstone, Chunying Yu, Sevan Hopyan, Jay S. Wunder, and Benjamin A. Alman. “PTHrP regulates growth plate chondrocyte differentiation and proliferation in a Gli3 dependent manner utilizing hedgehog ligand dependent and independent mechanisms.Dev Biol 305, no. 1 (May 1, 2007): 28–39. https://doi.org/10.1016/j.ydbio.2007.01.031.
Mau, Elaine, et al. “PTHrP regulates growth plate chondrocyte differentiation and proliferation in a Gli3 dependent manner utilizing hedgehog ligand dependent and independent mechanisms.Dev Biol, vol. 305, no. 1, May 2007, pp. 28–39. Pubmed, doi:10.1016/j.ydbio.2007.01.031.
Journal cover image

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

May 1, 2007

Volume

305

Issue

1

Start / End Page

28 / 39

Location

United States

Related Subject Headings

  • Zinc Finger Protein Gli3
  • Zinc Finger Protein Gli2
  • Signal Transduction
  • Protein Serine-Threonine Kinases
  • Parathyroid Hormone-Related Protein
  • Nerve Tissue Proteins
  • Mice, Knockout
  • Mice
  • Kruppel-Like Transcription Factors
  • In Situ Nick-End Labeling