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Beta-catenin signaling pathway is crucial for bone morphogenetic protein 2 to induce new bone formation.

Publication ,  Journal Article
Chen, Y; Whetstone, HC; Youn, A; Nadesan, P; Chow, ECY; Lin, AC; Alman, BA
Published in: J Biol Chem
January 5, 2007

Endochondral ossification is recapitulated during bone morphogenetic protein (BMP)-induced ectopic bone formation. Although BMP and beta-catenin have been investigated in bone development and in mesenchymal cells, how they interact in this process is not clear. We implanted recombinant BMP-2 into the muscle of mice to investigate the effect of beta-catenin signaling on BMP-induced in vivo endochondral bone formation. BMP-2 induced expression of several Wnt ligands and their receptors and also activated beta-catenin-mediated T cell factor-dependent transcriptional activity. An adenovirus expressing Dickkopf-1 (Dkk-1, an inhibitor of canonical Wnt pathway) inhibited beta-catenin signaling and endochondral bone formation. Interestingly, Dkk-1 inhibited both chondrogenesis and osteogenesis. Likewise, mice expressing conditional beta-catenin null alleles also displayed an inhibition of BMP-induced chondrogenesis and osteogenesis. This is in contrast to studies of embryonic skeletogenesis, which demonstrate that beta-catenin is required for osteogenesis but is dispensable for chondrogenesis. These findings suggest that embryonic development pathways are not always recapitulated during post-natal regenerative processes, and the biochemical pathways utilized to regulate cell differentiation may be different. During in vivo ectopic bone formation, BMP-2 induces beta-catenin-mediated signaling through Wnt ligands, and beta-catenin is required for both chondrogenesis and osteogenesis.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

January 5, 2007

Volume

282

Issue

1

Start / End Page

526 / 533

Location

United States

Related Subject Headings

  • beta Catenin
  • Transcription, Genetic
  • Signal Transduction
  • Recombinant Proteins
  • Osteoblasts
  • Mice, Transgenic
  • Mice
  • Male
  • Ligands
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chen, Y., Whetstone, H. C., Youn, A., Nadesan, P., Chow, E. C. Y., Lin, A. C., & Alman, B. A. (2007). Beta-catenin signaling pathway is crucial for bone morphogenetic protein 2 to induce new bone formation. J Biol Chem, 282(1), 526–533. https://doi.org/10.1074/jbc.M602700200
Chen, Yan, Heather C. Whetstone, Andrew Youn, Puviindran Nadesan, Edwin C. Y. Chow, Alvin C. Lin, and Benjamin A. Alman. “Beta-catenin signaling pathway is crucial for bone morphogenetic protein 2 to induce new bone formation.J Biol Chem 282, no. 1 (January 5, 2007): 526–33. https://doi.org/10.1074/jbc.M602700200.
Chen Y, Whetstone HC, Youn A, Nadesan P, Chow ECY, Lin AC, et al. Beta-catenin signaling pathway is crucial for bone morphogenetic protein 2 to induce new bone formation. J Biol Chem. 2007 Jan 5;282(1):526–33.
Chen, Yan, et al. “Beta-catenin signaling pathway is crucial for bone morphogenetic protein 2 to induce new bone formation.J Biol Chem, vol. 282, no. 1, Jan. 2007, pp. 526–33. Pubmed, doi:10.1074/jbc.M602700200.
Chen Y, Whetstone HC, Youn A, Nadesan P, Chow ECY, Lin AC, Alman BA. Beta-catenin signaling pathway is crucial for bone morphogenetic protein 2 to induce new bone formation. J Biol Chem. 2007 Jan 5;282(1):526–533.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

January 5, 2007

Volume

282

Issue

1

Start / End Page

526 / 533

Location

United States

Related Subject Headings

  • beta Catenin
  • Transcription, Genetic
  • Signal Transduction
  • Recombinant Proteins
  • Osteoblasts
  • Mice, Transgenic
  • Mice
  • Male
  • Ligands
  • Humans