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RGS10 is a selective activator of G alpha i GTPase activity.

Publication ,  Journal Article
Hunt, TW; Fields, TA; Casey, PJ; Peralta, EG
Published in: Nature
September 12, 1996

Polypeptides that define a protein family termed RGS (for regulators of G-protein signalling) are encoded by the SST2 gene of the yeast Saccharomyces cerevisiae, the EGL-10 gene of the nematode Caenorhabdatis elegans, and several related mammalian genes. Genetic studies in invertebrates and mammalian cell-transfection experiments indicate that RGS proteins negatively regulate signalling pathways involving seven transmembrane receptors and heterotrimeric G proteins. However, the biochemical mechanism by which RGS proteins control these pathways is unknown. Here we report the characterization of human RGS10, a member of this protein family. Co-immunoprecipitation studies demonstrate that RGS10 associates specifically with the activated forms of two related G-protein subunits, G alphai3, and G alphaz, but fails to interact with the structurally and functionally distinct G alphas subunit. In vitro assays with purified proteins indicate that RGS10 increases potently and selectively the GTP hydrolytic activity of several members of the G alphai family, including G alphai3, G alphaz, and G alpha0. These results demonstrate that RGS proteins can attenuate signalling pathways involving heterotrimeric G proteins by serving as GTPase-activating proteins for specific types of G alpha subunits.

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Published In

Nature

DOI

ISSN

0028-0836

Publication Date

September 12, 1996

Volume

383

Issue

6596

Start / End Page

175 / 177

Location

England

Related Subject Headings

  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Rats
  • RGS Proteins
  • Proteins
  • Precipitin Tests
  • Molecular Sequence Data
  • Hydrolysis
  • Humans
  • Hela Cells
 

Citation

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Hunt, T. W., Fields, T. A., Casey, P. J., & Peralta, E. G. (1996). RGS10 is a selective activator of G alpha i GTPase activity. Nature, 383(6596), 175–177. https://doi.org/10.1038/383175a0
Hunt, T. W., T. A. Fields, P. J. Casey, and E. G. Peralta. “RGS10 is a selective activator of G alpha i GTPase activity.Nature 383, no. 6596 (September 12, 1996): 175–77. https://doi.org/10.1038/383175a0.
Hunt TW, Fields TA, Casey PJ, Peralta EG. RGS10 is a selective activator of G alpha i GTPase activity. Nature. 1996 Sep 12;383(6596):175–7.
Hunt, T. W., et al. “RGS10 is a selective activator of G alpha i GTPase activity.Nature, vol. 383, no. 6596, Sept. 1996, pp. 175–77. Pubmed, doi:10.1038/383175a0.
Hunt TW, Fields TA, Casey PJ, Peralta EG. RGS10 is a selective activator of G alpha i GTPase activity. Nature. 1996 Sep 12;383(6596):175–177.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

September 12, 1996

Volume

383

Issue

6596

Start / End Page

175 / 177

Location

England

Related Subject Headings

  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Rats
  • RGS Proteins
  • Proteins
  • Precipitin Tests
  • Molecular Sequence Data
  • Hydrolysis
  • Humans
  • Hela Cells