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RNA interference of human papillomavirus type 18 E6 and E7 induces senescence in HeLa cells.

Publication ,  Journal Article
Hall, AHS; Alexander, KA
Published in: J Virol
May 2003

The human papillomavirus oncoproteins E6 and E7 promote cell proliferation and contribute to carcinogenesis by interfering with the activities of cellular tumor suppressors. We used a small interfering RNA molecule targeting the E7 region of the bicistronic E6 and E7 mRNA to induce RNA interference, thereby reducing expression of E6 and E7 in HeLa cells. RNA interference of E6 and E7 also inhibited cellular DNA synthesis and induced morphological and biochemical changes characteristic of cellular senescence. These results demonstrate that reducing E6 and E7 expression is sufficient to cause HeLa cells to become senescent.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

May 2003

Volume

77

Issue

10

Start / End Page

6066 / 6069

Location

United States

Related Subject Headings

  • Virology
  • RNA, Small Interfering
  • RNA, Messenger
  • RNA Interference
  • Papillomaviridae
  • Oncogene Proteins, Viral
  • Humans
  • Hela Cells
  • HeLa Cells
  • Gene Expression Regulation, Viral
 

Citation

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Hall, A. H. S., & Alexander, K. A. (2003). RNA interference of human papillomavirus type 18 E6 and E7 induces senescence in HeLa cells. J Virol, 77(10), 6066–6069. https://doi.org/10.1128/jvi.77.10.6066-6069.2003
Hall, Allison H. S., and Kenneth A. Alexander. “RNA interference of human papillomavirus type 18 E6 and E7 induces senescence in HeLa cells.J Virol 77, no. 10 (May 2003): 6066–69. https://doi.org/10.1128/jvi.77.10.6066-6069.2003.
Hall, Allison H. S., and Kenneth A. Alexander. “RNA interference of human papillomavirus type 18 E6 and E7 induces senescence in HeLa cells.J Virol, vol. 77, no. 10, May 2003, pp. 6066–69. Pubmed, doi:10.1128/jvi.77.10.6066-6069.2003.
Hall AHS, Alexander KA. RNA interference of human papillomavirus type 18 E6 and E7 induces senescence in HeLa cells. J Virol. 2003 May;77(10):6066–6069.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

May 2003

Volume

77

Issue

10

Start / End Page

6066 / 6069

Location

United States

Related Subject Headings

  • Virology
  • RNA, Small Interfering
  • RNA, Messenger
  • RNA Interference
  • Papillomaviridae
  • Oncogene Proteins, Viral
  • Humans
  • Hela Cells
  • HeLa Cells
  • Gene Expression Regulation, Viral