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Replication of the vesicular stomatitis virus genome in permissive and nonpermissive host cells.

Publication ,  Journal Article
Piwnica-Worms, H; Keene, JD
Published in: J Biol Chem
September 5, 1985

Permissive infections of BHK cells and nonpermissive infections of Raji cells were probed for the accumulation of vesicular stomatitis virus intracellular RNAs. In Raji cells, the onset of vesicular stomatitis virus transcription and replication was delayed when compared to BHK cells, and the accumulation of plus and minus sense leader RNAs was significantly reduced. In contrast, full length plus and minus strand replicative RNAs accumulated in Raji cells to levels approximately equivalent to those in BHK cells. In both cell types, approximately four times as many minus strands as plus strands were detected late in the infections. At 16 h postinfection, 12% of the total genomic RNA synthesized in BHK cells was packaged and released whereas only 0.8% was released from Raji cells. In addition, of those particles released by Raji cells, only 1% were infectious whereas 77% of those released by BHK cells were infectious. The virions released from both cell types contained similar amounts of the five viral proteins, however. Analysis of virions from Raji cells revealed a faster electrophoretic mobility of the glycoprotein than the glycoprotein in virions released from BHK cells. These results suggest that Raji cells may be restricted in their ability to support a complete infection at the level of virus assembly rather than at the level of RNA replication.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

September 5, 1985

Volume

260

Issue

19

Start / End Page

10503 / 10511

Location

United States

Related Subject Headings

  • Virus Replication
  • Viral Proteins
  • Vesicular stomatitis Indiana virus
  • Transcription, Genetic
  • RNA, Viral
  • Kidney
  • Humans
  • Genes, Viral
  • DNA Replication
  • Cricetinae
 

Citation

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MLA
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Piwnica-Worms, H., & Keene, J. D. (1985). Replication of the vesicular stomatitis virus genome in permissive and nonpermissive host cells. J Biol Chem, 260(19), 10503–10511.
Piwnica-Worms, H., and J. D. Keene. “Replication of the vesicular stomatitis virus genome in permissive and nonpermissive host cells.J Biol Chem 260, no. 19 (September 5, 1985): 10503–11.
Piwnica-Worms H, Keene JD. Replication of the vesicular stomatitis virus genome in permissive and nonpermissive host cells. J Biol Chem. 1985 Sep 5;260(19):10503–11.
Piwnica-Worms, H., and J. D. Keene. “Replication of the vesicular stomatitis virus genome in permissive and nonpermissive host cells.J Biol Chem, vol. 260, no. 19, Sept. 1985, pp. 10503–11.
Piwnica-Worms H, Keene JD. Replication of the vesicular stomatitis virus genome in permissive and nonpermissive host cells. J Biol Chem. 1985 Sep 5;260(19):10503–10511.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

September 5, 1985

Volume

260

Issue

19

Start / End Page

10503 / 10511

Location

United States

Related Subject Headings

  • Virus Replication
  • Viral Proteins
  • Vesicular stomatitis Indiana virus
  • Transcription, Genetic
  • RNA, Viral
  • Kidney
  • Humans
  • Genes, Viral
  • DNA Replication
  • Cricetinae