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Transfer RNAs associated with vesicular stomatitis virus.

Publication ,  Journal Article
Isaac, CL; Keene, JD
Published in: J Gen Virol
September 1981

The predominant RNAs in purified VSV particles are 42S and 4S in size. The 4S RNA is host transfer RNA that did not incorporate detectable radiolabel during VSV infection and was detected by in vitro labelling. Surprisingly, when BHK cells were prelabelled for 30 to 54 h before infection, the incorporation of [3H]uridine and [32P]orthophosphate into virus tRNAs remained very low and virus tRNAs were found to have a 5- to 15-fold lower specific activity than the total host tRNA, the value depending, in part, upon the period of prelabelling. Two-dimensional gel electrophoresis and partial sequence analysis indicated that the virus tRNAs include most species of host tRNA and no singly predominant species was apparent. Transfer RNAs are packaged by several enveloped viruses, but we have not found 4S RNA in reovirus, which lacks an envelope. We suggest that VSV contains a membrane-associated population of tRNA which has a slower rate of turnover than the total population of cellular tRNA.

Duke Scholars

Published In

J Gen Virol

DOI

ISSN

0022-1317

Publication Date

September 1981

Volume

56

Issue

Pt 1

Start / End Page

141 / 151

Location

England

Related Subject Headings

  • Virology
  • Vesicular stomatitis Indiana virus
  • RNA, Viral
  • RNA, Transfer
  • Electrophoresis, Polyacrylamide Gel
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
 

Citation

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Isaac, C. L., & Keene, J. D. (1981). Transfer RNAs associated with vesicular stomatitis virus. J Gen Virol, 56(Pt 1), 141–151. https://doi.org/10.1099/0022-1317-56-1-141
Isaac, C. L., and J. D. Keene. “Transfer RNAs associated with vesicular stomatitis virus.J Gen Virol 56, no. Pt 1 (September 1981): 141–51. https://doi.org/10.1099/0022-1317-56-1-141.
Isaac CL, Keene JD. Transfer RNAs associated with vesicular stomatitis virus. J Gen Virol. 1981 Sep;56(Pt 1):141–51.
Isaac, C. L., and J. D. Keene. “Transfer RNAs associated with vesicular stomatitis virus.J Gen Virol, vol. 56, no. Pt 1, Sept. 1981, pp. 141–51. Pubmed, doi:10.1099/0022-1317-56-1-141.
Isaac CL, Keene JD. Transfer RNAs associated with vesicular stomatitis virus. J Gen Virol. 1981 Sep;56(Pt 1):141–151.

Published In

J Gen Virol

DOI

ISSN

0022-1317

Publication Date

September 1981

Volume

56

Issue

Pt 1

Start / End Page

141 / 151

Location

England

Related Subject Headings

  • Virology
  • Vesicular stomatitis Indiana virus
  • RNA, Viral
  • RNA, Transfer
  • Electrophoresis, Polyacrylamide Gel
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences