Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

The identification of a second actin-binding region in spinophilin/neurabin II.

Publication ,  Journal Article
Barnes, AP; Smith, FD; VanDongen, HM; VanDongen, AMJ; Milgram, SL
Published in: Brain Res Mol Brain Res
May 19, 2004

Spinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons. Previously, the actin-binding domain (ABD) of spinophilin was localized to a domain between amino acids (aa) 1 and 154. In a mass spectrometry screen for spinophilin-binding proteins, we have identified an additional actin-binding region between aa 151 and 282. F-actin co-sedimentation and GST affinity chromotography experiments further substantiate this result. Phalloidin staining of Rat2 fibroblasts transiently expressing GFP-spinophilin deletion constructs indicates co-localization with a subset of actin. Regions of spinophilin that lack the revised ABD (aa 1-230) do not co-localize with phalloidin-labeled actin, suggesting that the actin-binding domain contributes to directing the subcellular distribution of spinophilin. Targeting experiments using primary hippocampal cultures indicate that only the first actin-binding site contributes to dendritic spine localization. The second ABD targets to spines inefficiently and thus may interact with and affect actin filaments in a different manner than the first ABD.

Duke Scholars

Published In

Brain Res Mol Brain Res

DOI

ISSN

0169-328X

Publication Date

May 19, 2004

Volume

124

Issue

2

Start / End Page

105 / 113

Location

Netherlands

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Recombinant Fusion Proteins
  • Rats
  • Protein Structure, Tertiary
  • Protein Binding
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Microfilament Proteins
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Barnes, A. P., Smith, F. D., VanDongen, H. M., VanDongen, A. M. J., & Milgram, S. L. (2004). The identification of a second actin-binding region in spinophilin/neurabin II. Brain Res Mol Brain Res, 124(2), 105–113. https://doi.org/10.1016/j.molbrainres.2003.12.020
Barnes, Anthony P., F Donelson Smith, Hendrika M. VanDongen, Antonius M. J. VanDongen, and Sharon L. Milgram. “The identification of a second actin-binding region in spinophilin/neurabin II.Brain Res Mol Brain Res 124, no. 2 (May 19, 2004): 105–13. https://doi.org/10.1016/j.molbrainres.2003.12.020.
Barnes AP, Smith FD, VanDongen HM, VanDongen AMJ, Milgram SL. The identification of a second actin-binding region in spinophilin/neurabin II. Brain Res Mol Brain Res. 2004 May 19;124(2):105–13.
Barnes, Anthony P., et al. “The identification of a second actin-binding region in spinophilin/neurabin II.Brain Res Mol Brain Res, vol. 124, no. 2, May 2004, pp. 105–13. Pubmed, doi:10.1016/j.molbrainres.2003.12.020.
Barnes AP, Smith FD, VanDongen HM, VanDongen AMJ, Milgram SL. The identification of a second actin-binding region in spinophilin/neurabin II. Brain Res Mol Brain Res. 2004 May 19;124(2):105–113.
Journal cover image

Published In

Brain Res Mol Brain Res

DOI

ISSN

0169-328X

Publication Date

May 19, 2004

Volume

124

Issue

2

Start / End Page

105 / 113

Location

Netherlands

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Recombinant Fusion Proteins
  • Rats
  • Protein Structure, Tertiary
  • Protein Binding
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Microfilament Proteins
  • Humans