piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice.
Publication
, Journal Article
Li, R; Zhuang, Y; Han, M; Xu, T; Wu, X
Published in: Dis Model Mech
May 2013
The stable genomic integration and expression of a large transgene is a major hurdle in gene therapy. We show that the modified piggyBac (PB) transposon system can be used to introduce a 207 kb genomic DNA fragment containing the RORγ/γt locus into human cells and mice. PB-mediated transgenesis results in a single copy of a stably inherited and expressed transgene. These results indicate that PB could serve as an effective high-capacity vector for functional analysis of the mammalian genome and for gene therapy in human cells.
Duke Scholars
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Published In
Dis Model Mech
DOI
EISSN
1754-8411
Publication Date
May 2013
Volume
6
Issue
3
Start / End Page
828 / 833
Location
England
Related Subject Headings
- Transgenes
- Mutagenesis, Insertional
- Molecular Sequence Data
- Mice
- Humans
- HEK293 Cells
- Genetic Vectors
- Genetic Therapy
- Gene Transfer Techniques
- Embryonic Stem Cells
Citation
APA
Chicago
ICMJE
MLA
NLM
Li, R., Zhuang, Y., Han, M., Xu, T., & Wu, X. (2013). piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice. Dis Model Mech, 6(3), 828–833. https://doi.org/10.1242/dmm.010827
Li, Rongbo, Yuan Zhuang, Min Han, Tian Xu, and Xiaohui Wu. “piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice.” Dis Model Mech 6, no. 3 (May 2013): 828–33. https://doi.org/10.1242/dmm.010827.
Li R, Zhuang Y, Han M, Xu T, Wu X. piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice. Dis Model Mech. 2013 May;6(3):828–33.
Li, Rongbo, et al. “piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice.” Dis Model Mech, vol. 6, no. 3, May 2013, pp. 828–33. Pubmed, doi:10.1242/dmm.010827.
Li R, Zhuang Y, Han M, Xu T, Wu X. piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice. Dis Model Mech. 2013 May;6(3):828–833.
Published In
Dis Model Mech
DOI
EISSN
1754-8411
Publication Date
May 2013
Volume
6
Issue
3
Start / End Page
828 / 833
Location
England
Related Subject Headings
- Transgenes
- Mutagenesis, Insertional
- Molecular Sequence Data
- Mice
- Humans
- HEK293 Cells
- Genetic Vectors
- Genetic Therapy
- Gene Transfer Techniques
- Embryonic Stem Cells