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A high-performance liquid chromatography method for the quantification of cysmethynil, an inhibitor of isoprenylcysteine carboxylmethyl transferase, in mouse plasma.

Publication ,  Journal Article
Wang, M; Khoo, YM; Zhou, J; Casey, P; Lee, HS
Published in: J Chromatogr B Analyt Technol Biomed Life Sci
February 15, 2009

Cysmethynil, a newly identified small molecule inhibitor of isoprenylcysteine carboxylmethyl transferase (Icmt) is involved in the post-translational modification of CaaX proteins. Cysmethynil causes cell death in many human cancer cells in vitro, and inhibits tumor growth in the xenograft mouse model in vivo. A HPLC method for the quantification of cysmethynil in mouse plasma was developed and validated. The lower limit of quantification of this method was 0.01microg/ml. Inter- and intra-day variability ranged from 0.38-8.5% and accuracy was between 86% and 98%. This sensitive method was used to quantify cysmethynil in plasma of mice after intraperitoneal dosing for preliminary pharmacokinetic studies.

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Published In

J Chromatogr B Analyt Technol Biomed Life Sci

DOI

ISSN

1570-0232

Publication Date

February 15, 2009

Volume

877

Issue

5-6

Start / End Page

553 / 557

Location

Netherlands

Related Subject Headings

  • Time Factors
  • Temperature
  • Reproducibility of Results
  • Protein Methyltransferases
  • Mice, Inbred BALB C
  • Mice
  • Indoles
  • Freezing
  • Enzyme Inhibitors
  • Drug Stability
 

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Wang, M., Khoo, Y. M., Zhou, J., Casey, P., & Lee, H. S. (2009). A high-performance liquid chromatography method for the quantification of cysmethynil, an inhibitor of isoprenylcysteine carboxylmethyl transferase, in mouse plasma. J Chromatogr B Analyt Technol Biomed Life Sci, 877(5–6), 553–557. https://doi.org/10.1016/j.jchromb.2008.12.067
Wang, Mei, Yok Moi Khoo, Jin Zhou, Patrick Casey, and How Sung Lee. “A high-performance liquid chromatography method for the quantification of cysmethynil, an inhibitor of isoprenylcysteine carboxylmethyl transferase, in mouse plasma.J Chromatogr B Analyt Technol Biomed Life Sci 877, no. 5–6 (February 15, 2009): 553–57. https://doi.org/10.1016/j.jchromb.2008.12.067.
Wang M, Khoo YM, Zhou J, Casey P, Lee HS. A high-performance liquid chromatography method for the quantification of cysmethynil, an inhibitor of isoprenylcysteine carboxylmethyl transferase, in mouse plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Feb 15;877(5–6):553–7.
Wang, Mei, et al. “A high-performance liquid chromatography method for the quantification of cysmethynil, an inhibitor of isoprenylcysteine carboxylmethyl transferase, in mouse plasma.J Chromatogr B Analyt Technol Biomed Life Sci, vol. 877, no. 5–6, Feb. 2009, pp. 553–57. Pubmed, doi:10.1016/j.jchromb.2008.12.067.
Wang M, Khoo YM, Zhou J, Casey P, Lee HS. A high-performance liquid chromatography method for the quantification of cysmethynil, an inhibitor of isoprenylcysteine carboxylmethyl transferase, in mouse plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Feb 15;877(5–6):553–557.
Journal cover image

Published In

J Chromatogr B Analyt Technol Biomed Life Sci

DOI

ISSN

1570-0232

Publication Date

February 15, 2009

Volume

877

Issue

5-6

Start / End Page

553 / 557

Location

Netherlands

Related Subject Headings

  • Time Factors
  • Temperature
  • Reproducibility of Results
  • Protein Methyltransferases
  • Mice, Inbred BALB C
  • Mice
  • Indoles
  • Freezing
  • Enzyme Inhibitors
  • Drug Stability