
Inhibition of purified p21ras farnesyl:protein transferase by Cys-AAX tetrapeptides.
We report the identification, purification, and characterization of a farnesyl:protein transferase that transfers the farnesyl moiety from farnesyl pyrophosphate to a cysteine in p21ras proteins. The enzyme was purified approximately 60,000-fold from rat brain cytosol through use of a chromatography step based on the enzyme's ability to bind to a hexapeptide containing the consensus sequence (Cys-AAX) for farnesylation. The purified enzyme migrated on gel filtration chromatography with an apparent molecular weight of 70,000-100,000. High resolution SDS-polyacrylamide gels showed two closely spaced approximately 50 kd protein bands in the final preparation. The enzyme was inhibited competitively by peptides as short as 4 residues that contained the Cys-AAX motif. These peptides acted as alternative substrates that competed with p21H-ras for farnesylation. Effective peptides included the COOH-terminal sequences of all known p21ras proteins as well as those of lamin A and B.
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Related Subject Headings
- Transferases
- Structure-Activity Relationship
- Sesquiterpenes
- Rats, Inbred Strains
- Rats
- Proto-Oncogene Proteins p21(ras)
- Proto-Oncogene Proteins
- Protein-Tyrosine Kinases
- Polyisoprenyl Phosphates
- Oligopeptides
Citation

Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transferases
- Structure-Activity Relationship
- Sesquiterpenes
- Rats, Inbred Strains
- Rats
- Proto-Oncogene Proteins p21(ras)
- Proto-Oncogene Proteins
- Protein-Tyrosine Kinases
- Polyisoprenyl Phosphates
- Oligopeptides