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G-CSF induces a potentially tolerant gene and immunophenotype profile in T cells in vivo.

Publication ,  Journal Article
Toh, HC; Sun, L; Soe, Y; Wu, Y; Phoon, YP; Chia, WK; Wu, J; Wong, KY; Tan, P
Published in: Clin Immunol
July 2009

G-CSF can induce functional immune tolerance in man. In this study, purified T cells from G-CSF-mobilized peripheral blood stem cell (PBSC) donors were analysed by gene expression profiling and immunophenotyping. Results suggested a predominantly immune tolerant profile with upregulation of genes related to Th2 and Treg cells, downregulation of genes associated with Th1 cells, cytotoxicity, antigen presentation and graft-versus-host disease (GVHD) and overexpression of negative regulators of Th17 differentiation. Immunophenotyping revealed that during G-CSF exposure donors had reduced levels of T cells with a Th17 phenotype (CD4+IL-17A+CCR6+IL-23R+), more than three times lower compared to normal controls. G-CSF also led to increased levels of CD4+CD25highCD45RO+ Treg cells. Furthermore, mRNA levels of RORgammat, a Th17-specific transcription factor, decreased in T cells isolated from G-CSF-mobilized PBSC harvests. Th17 cells have been implicated in autoimmune diseases and GVHD pathophysiology. Our study is the first to report the effect of G-CSF on the Th17 subpopulation.

Duke Scholars

Published In

Clin Immunol

DOI

EISSN

1521-7035

Publication Date

July 2009

Volume

132

Issue

1

Start / End Page

83 / 92

Location

United States

Related Subject Headings

  • Th2 Cells
  • Th1 Cells
  • T-Lymphocytes, Regulatory
  • T-Lymphocytes, Helper-Inducer
  • T-Lymphocytes
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Thyroid Hormone
  • Receptors, Retinoic Acid
  • Nuclear Receptor Subfamily 1, Group F, Member 3
  • Interleukin-17
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Toh, H. C., Sun, L., Soe, Y., Wu, Y., Phoon, Y. P., Chia, W. K., … Tan, P. (2009). G-CSF induces a potentially tolerant gene and immunophenotype profile in T cells in vivo. Clin Immunol, 132(1), 83–92. https://doi.org/10.1016/j.clim.2009.03.509
Toh, Han C., Li Sun, Yatanar Soe, Yonghui Wu, Yee P. Phoon, Whay K. Chia, Jeanie Wu, Kee Y. Wong, and Patrick Tan. “G-CSF induces a potentially tolerant gene and immunophenotype profile in T cells in vivo.Clin Immunol 132, no. 1 (July 2009): 83–92. https://doi.org/10.1016/j.clim.2009.03.509.
Toh HC, Sun L, Soe Y, Wu Y, Phoon YP, Chia WK, et al. G-CSF induces a potentially tolerant gene and immunophenotype profile in T cells in vivo. Clin Immunol. 2009 Jul;132(1):83–92.
Toh, Han C., et al. “G-CSF induces a potentially tolerant gene and immunophenotype profile in T cells in vivo.Clin Immunol, vol. 132, no. 1, July 2009, pp. 83–92. Pubmed, doi:10.1016/j.clim.2009.03.509.
Toh HC, Sun L, Soe Y, Wu Y, Phoon YP, Chia WK, Wu J, Wong KY, Tan P. G-CSF induces a potentially tolerant gene and immunophenotype profile in T cells in vivo. Clin Immunol. 2009 Jul;132(1):83–92.
Journal cover image

Published In

Clin Immunol

DOI

EISSN

1521-7035

Publication Date

July 2009

Volume

132

Issue

1

Start / End Page

83 / 92

Location

United States

Related Subject Headings

  • Th2 Cells
  • Th1 Cells
  • T-Lymphocytes, Regulatory
  • T-Lymphocytes, Helper-Inducer
  • T-Lymphocytes
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Thyroid Hormone
  • Receptors, Retinoic Acid
  • Nuclear Receptor Subfamily 1, Group F, Member 3
  • Interleukin-17