Scholars@Duke publication: Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts

Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts

Publication , Journal Article

Swamy, SMK; Tan, P; Zhu, YZ; Lu, J; Achuth, HN; Moochhala, S

Published in: Biochemical and Biophysical Research Communications

2004

Wound healing is a complex process involving a number of related genes and receptors. Using cDNA microarrays, we explored the global gene expression profile of phenytoin (20μg/ml) induced changes to human dermal fibroblasts. Microarray data analysis revealed ∼1500 genes were differentially expressed by 2.5-fold. At 3, 6, 12, and 24h, the transcripts of the major growth factors involved in wound healing and their receptors were increased. This was further confirmed by RT-PCR. Genes encoding other proteins with roles in signal transduction (NFκB), extracellular matrix (MMP1) including type I collagen, fibronectin, and laminin were strongly induced at 6h and onwards. Genes involved in cell cycle regulation (CCND1 and CDKN1A) were down-regulated consistent with our finding that phenytoin per se did not have cell proliferation activity. Notably, phenytoin accelerates the autocrine and paracrine activity of growth factors by up-regulating the related receptors. © 2003 Elsevier Inc. All rights reserved.

Duke Scholars

Author Patrick Tan Medicine, Medical Oncology

Published In

Biochemical and Biophysical Research Communications

DOI

10.1016/j.bbrc.2003.12.146

Publication Date

2004

Volume

314

Issue

Start / End Page

661 / 666

Related Subject Headings

Biochemistry & Molecular Biology
3404 Medicinal and biomolecular chemistry
3101 Biochemistry and cell biology
1101 Medical Biochemistry and Metabolomics
0601 Biochemistry and Cell Biology
0304 Medicinal and Biomolecular Chemistry

Citation

APA

Chicago

ICMJE

MLA

NLM

Swamy, S. M. K., Tan, P., Zhu, Y. Z., Lu, J., Achuth, H. N., & Moochhala, S. (2004). Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts. Biochemical and Biophysical Research Communications, 314(3), 661–666. https://doi.org/10.1016/j.bbrc.2003.12.146

Swamy, S. M. K., P. Tan, Y. Z. Zhu, J. Lu, H. N. Achuth, and S. Moochhala. “Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts.” Biochemical and Biophysical Research Communications 314, no. 3 (2004): 661–66. https://doi.org/10.1016/j.bbrc.2003.12.146.

Swamy SMK, Tan P, Zhu YZ, Lu J, Achuth HN, Moochhala S. Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts. Biochemical and Biophysical Research Communications. 2004;314(3):661–6.

Swamy, S. M. K., et al. “Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts.” Biochemical and Biophysical Research Communications, vol. 314, no. 3, 2004, pp. 661–66. Scival, doi:10.1016/j.bbrc.2003.12.146.

Published In

Biochemical and Biophysical Research Communications

DOI

10.1016/j.bbrc.2003.12.146

Publication Date

2004

Volume

314

Issue

Start / End Page

661 / 666

Related Subject Headings

Biochemistry & Molecular Biology
3404 Medicinal and biomolecular chemistry
3101 Biochemistry and cell biology
1101 Medical Biochemistry and Metabolomics
0601 Biochemistry and Cell Biology
0304 Medicinal and Biomolecular Chemistry