Skip to main content
release_alert
Welcome to the new Scholars 3.0! Read about new features and let us know what you think.
cancel
Journal cover image

Progesterone receptor membrane component 1 as the mediator of the inhibitory effect of progestins on cytokine-induced matrix metalloproteinase 9 activity in vitro.

Publication ,  Journal Article
Allen, TK; Feng, L; Grotegut, CA; Murtha, AP
Published in: Reprod Sci
February 2014

Progesterone (P4) and the progestin, 17α-hydroxyprogesterone caproate, are clinically used to prevent preterm births (PTBs); however, their mechanism of action remains unclear. Cytokine-induced matrix metalloproteinase 9 (MMP-9) activity plays a key role in preterm premature rupture of the membranes and PTB. We demonstrated that the primary chorion cells and the HTR8/SVneo cells (cytotrophoblast cell line) do not express the classical progesterone receptor (PGR) but instead a novel progesterone receptor, progesterone receptor membrane component 1 (PGRMC1), whose role remains unclear. Using HTR8/SVneo cells in culture, we further demonstrated that 6 hours pretreatment with medroxyprogesterone acetate (MPA) and dexamethasone (Dex) but not P4 or 17α-hydroxyprogesterone hexanoate significantly attenuated tumor necrosis factor α-induced MMP-9 activity after a 24-hour incubation period. The inhibitory effect of MPA, but not Dex, was attenuated when PGRMC1 expression was successfully reduced by PGRMC1 small interfering RNA. Our findings highlight a possible novel role of PGRMC1 in mediating the effects of MPA and in modulating cytokine-induced MMP-9 activity in cytotrophoblast cells in vitro.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

February 2014

Volume

21

Issue

2

Start / End Page

260 / 268

Location

United States

Related Subject Headings

  • Receptors, Progesterone
  • Progestins
  • Obstetrics & Reproductive Medicine
  • Membrane Proteins
  • Matrix Metalloproteinase 9
  • Humans
  • Female
  • Extraembryonic Membranes
  • Enzyme Activation
  • Cytokines
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Allen, T. K., Feng, L., Grotegut, C. A., & Murtha, A. P. (2014). Progesterone receptor membrane component 1 as the mediator of the inhibitory effect of progestins on cytokine-induced matrix metalloproteinase 9 activity in vitro. Reprod Sci, 21(2), 260–268. https://doi.org/10.1177/1933719113493514
Allen, Terrence K., Liping Feng, Chad A. Grotegut, and Amy P. Murtha. “Progesterone receptor membrane component 1 as the mediator of the inhibitory effect of progestins on cytokine-induced matrix metalloproteinase 9 activity in vitro.Reprod Sci 21, no. 2 (February 2014): 260–68. https://doi.org/10.1177/1933719113493514.
Allen, Terrence K., et al. “Progesterone receptor membrane component 1 as the mediator of the inhibitory effect of progestins on cytokine-induced matrix metalloproteinase 9 activity in vitro.Reprod Sci, vol. 21, no. 2, Feb. 2014, pp. 260–68. Pubmed, doi:10.1177/1933719113493514.
Journal cover image

Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

February 2014

Volume

21

Issue

2

Start / End Page

260 / 268

Location

United States

Related Subject Headings

  • Receptors, Progesterone
  • Progestins
  • Obstetrics & Reproductive Medicine
  • Membrane Proteins
  • Matrix Metalloproteinase 9
  • Humans
  • Female
  • Extraembryonic Membranes
  • Enzyme Activation
  • Cytokines