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Development of a method to isolate circulating tumor cells using mesenchymal-based capture.

Publication ,  Journal Article
Bitting, RL; Boominathan, R; Rao, C; Kemeny, G; Foulk, B; Garcia-Blanco, MA; Connelly, M; Armstrong, AJ
Published in: Methods
December 1, 2013

Epithelial tumor cells can become mesenchymal cells and vice versa via phenotypic transitions, a process known as epithelial plasticity. We postulate that during the process of metastasis, circulating tumor cells (CTCs) lose their epithelial phenotype and acquire a mesenchymal phenotype that may not be sufficiently captured by existing epithelial-based CTC technologies. We report here on the development of a novel CTC capture method, based on the biology of epithelial plasticity, which isolates cells based on OB-cadherin cell surface expression. Using this mesenchymal-based assay, OB-cadherin cellular events are detectable in men with metastatic prostate cancer and are less common in healthy volunteers. This method may complement existing epithelial-based methods and may be particularly useful in patients with bone metastases.

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Published In

Methods

DOI

EISSN

1095-9130

Publication Date

December 1, 2013

Volume

64

Issue

2

Start / End Page

129 / 136

Location

United States

Related Subject Headings

  • Prostatic Neoplasms
  • Neoplastic Cells, Circulating
  • Mesenchymal Stem Cells
  • Male
  • Humans
  • Epithelial-Mesenchymal Transition
  • Epithelial Cells
  • Cell Separation
  • Cell Line, Tumor
  • Cadherins
 

Citation

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Bitting, R. L., Boominathan, R., Rao, C., Kemeny, G., Foulk, B., Garcia-Blanco, M. A., … Armstrong, A. J. (2013). Development of a method to isolate circulating tumor cells using mesenchymal-based capture. Methods, 64(2), 129–136. https://doi.org/10.1016/j.ymeth.2013.06.034
Bitting, Rhonda L., Rengasamy Boominathan, Chandra Rao, Gabor Kemeny, Brad Foulk, Mariano A. Garcia-Blanco, Mark Connelly, and Andrew J. Armstrong. “Development of a method to isolate circulating tumor cells using mesenchymal-based capture.Methods 64, no. 2 (December 1, 2013): 129–36. https://doi.org/10.1016/j.ymeth.2013.06.034.
Bitting RL, Boominathan R, Rao C, Kemeny G, Foulk B, Garcia-Blanco MA, et al. Development of a method to isolate circulating tumor cells using mesenchymal-based capture. Methods. 2013 Dec 1;64(2):129–36.
Bitting, Rhonda L., et al. “Development of a method to isolate circulating tumor cells using mesenchymal-based capture.Methods, vol. 64, no. 2, Dec. 2013, pp. 129–36. Pubmed, doi:10.1016/j.ymeth.2013.06.034.
Bitting RL, Boominathan R, Rao C, Kemeny G, Foulk B, Garcia-Blanco MA, Connelly M, Armstrong AJ. Development of a method to isolate circulating tumor cells using mesenchymal-based capture. Methods. 2013 Dec 1;64(2):129–136.

Published In

Methods

DOI

EISSN

1095-9130

Publication Date

December 1, 2013

Volume

64

Issue

2

Start / End Page

129 / 136

Location

United States

Related Subject Headings

  • Prostatic Neoplasms
  • Neoplastic Cells, Circulating
  • Mesenchymal Stem Cells
  • Male
  • Humans
  • Epithelial-Mesenchymal Transition
  • Epithelial Cells
  • Cell Separation
  • Cell Line, Tumor
  • Cadherins