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Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers.

Publication ,  Journal Article
Kurzbach, D; Hassouneh, W; McDaniel, JR; Jaumann, EA; Chilkoti, A; Hinderberger, D
Published in: Journal of the American Chemical Society
July 2013

It is shown that hydrophilic (backbone) and hydrophobic (side chain) hydration layers of elastin-like polypeptides (ELPs), a class of stimulus responsive peptide polymers that exhibit lower critical solution temperature (LCST) phase transition behavior, can exist in a coupled and decoupled state. The decoupled hydration state consists of hydrophobic and hydrophilic hydration layers that respond independently to temperature, while the coupled hydration state is characterized by a common, cooperative dehydration of both hydration layers. It is further shown that the primary sequence of an ELP can be tuned to exhibit either of the hydration layer coupling modes. Charged side chains lead to decoupling, while strongly hydrophobic side chains trigger stronger interaction between hydrophilic and hydrophobic hydration, leading to coupling of both layers. Further, for aprotic residues this coupling is fostered by decreasing bulkiness of hydrophobic side chains due to larger hydration numbers and water molecules mediating coupling between side chain and backbone hydration shells. For coupled hydration shells, the LCST phase transition characterized by spin probing continuous wave electron paramagnetic resonance spectroscopy is reminiscent of a first-order process even on nanoscopic length scales. In contrast, analogous synthetic polymers exhibit nanoscale phase transitions over a broad temperature range, indicating that their nanoscale phase behavior is not of first order. Hence, our results indicate that ELPs are the first identified class of polymers that exhibit a first-order inverse phase transition on nanoscopic length scales. These results may also provide insights into the role of hydration layers in governing the structure-function relationship of intrinsically disordered proteins.

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Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

July 2013

Volume

135

Issue

30

Start / End Page

11299 / 11308

Related Subject Headings

  • Water
  • Temperature
  • Phase Transition
  • Peptides
  • Hydrophobic and Hydrophilic Interactions
  • General Chemistry
  • Elastin
  • 40 Engineering
  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

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ICMJE
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Kurzbach, D., Hassouneh, W., McDaniel, J. R., Jaumann, E. A., Chilkoti, A., & Hinderberger, D. (2013). Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers. Journal of the American Chemical Society, 135(30), 11299–11308. https://doi.org/10.1021/ja4047872
Kurzbach, Dennis, Wafa Hassouneh, Jonathan R. McDaniel, Eva A. Jaumann, Ashutosh Chilkoti, and Dariush Hinderberger. “Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers.Journal of the American Chemical Society 135, no. 30 (July 2013): 11299–308. https://doi.org/10.1021/ja4047872.
Kurzbach D, Hassouneh W, McDaniel JR, Jaumann EA, Chilkoti A, Hinderberger D. Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers. Journal of the American Chemical Society. 2013 Jul;135(30):11299–308.
Kurzbach, Dennis, et al. “Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers.Journal of the American Chemical Society, vol. 135, no. 30, July 2013, pp. 11299–308. Epmc, doi:10.1021/ja4047872.
Kurzbach D, Hassouneh W, McDaniel JR, Jaumann EA, Chilkoti A, Hinderberger D. Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers. Journal of the American Chemical Society. 2013 Jul;135(30):11299–11308.
Journal cover image

Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

July 2013

Volume

135

Issue

30

Start / End Page

11299 / 11308

Related Subject Headings

  • Water
  • Temperature
  • Phase Transition
  • Peptides
  • Hydrophobic and Hydrophilic Interactions
  • General Chemistry
  • Elastin
  • 40 Engineering
  • 34 Chemical sciences
  • 03 Chemical Sciences