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Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization.

Publication ,  Journal Article
Khan, MN; Tjong, V; Chilkoti, A; Zharnikov, M
Published in: The journal of physical chemistry. B
August 2013

We used a combination of synchrotron-based X-ray photoelectron spectroscopy (XPS) and angle-resolved near-edge X-ray absorption fine structure (NEXAFS) spectroscopy to study the chemical integrity, purity, and possible internal alignment of single-strand (ss) adenine deoxynucleotide (poly(A)) DNA brushes. The brushes were synthesized by surface-initiated enzymatic polymerization (SIEP) on a 25-mer of adenine self-assembled monolayer (SAM) on gold (A25-SH), wherein the terminal 3'-OH of the A25-SH serve as the initiation sites for SIEP of poly(A). XPS and NEXAFS spectra of poly(A) brushes were found to be almost identical to those of A25-SH initiator, with no unambiguous traces of contamination. Apart from the well-defined chemical integrity and contamination-free character, the brushes were found to have a high degree of orientational order, with an upright orientation of individual strands, despite their large thickness up to ~55 nm, that corresponds to a chain length of at least several hundred nucleotides for individual ssDNA molecules. The orientational order exhibited by these poly(A) DNA brushes, mediated presumably by base stacking, was found to be independent of the brush thickness as long as the packing density was high enough. The well-defined character and orientational ordering of the ssDNA brushes make them a potentially promising system for different applications.

Duke Scholars

Published In

The journal of physical chemistry. B

DOI

EISSN

1520-5207

ISSN

1520-6106

Publication Date

August 2013

Volume

117

Issue

34

Start / End Page

9929 / 9938

Related Subject Headings

  • Surface Properties
  • Polymerization
  • Poly A
  • Photoelectron Spectroscopy
  • Gold
  • Enzymes
  • DNA, Single-Stranded
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
 

Citation

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Khan, M. N., Tjong, V., Chilkoti, A., & Zharnikov, M. (2013). Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization. The Journal of Physical Chemistry. B, 117(34), 9929–9938. https://doi.org/10.1021/jp404774x
Khan, M Nuruzzaman, Vinalia Tjong, Ashutosh Chilkoti, and Michael Zharnikov. “Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization.The Journal of Physical Chemistry. B 117, no. 34 (August 2013): 9929–38. https://doi.org/10.1021/jp404774x.
Khan MN, Tjong V, Chilkoti A, Zharnikov M. Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization. The journal of physical chemistry B. 2013 Aug;117(34):9929–38.
Khan, M. Nuruzzaman, et al. “Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization.The Journal of Physical Chemistry. B, vol. 117, no. 34, Aug. 2013, pp. 9929–38. Epmc, doi:10.1021/jp404774x.
Khan MN, Tjong V, Chilkoti A, Zharnikov M. Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization. The journal of physical chemistry B. 2013 Aug;117(34):9929–9938.
Journal cover image

Published In

The journal of physical chemistry. B

DOI

EISSN

1520-5207

ISSN

1520-6106

Publication Date

August 2013

Volume

117

Issue

34

Start / End Page

9929 / 9938

Related Subject Headings

  • Surface Properties
  • Polymerization
  • Poly A
  • Photoelectron Spectroscopy
  • Gold
  • Enzymes
  • DNA, Single-Stranded
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences