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WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs.

Publication ,  Journal Article
Jiang, W; Zhang, D; Bursac, N; Zhang, Y
Published in: Stem cell reports
January 2013

Generation of functional cells from human pluripotent stem cells (PSCs) through in vitro differentiation is a promising approach for drug screening and cell therapy. However, the observed large and unavoidable variation in the differentiation potential of different human embryonic stem cell (hESC)/induced PSC (iPSC) lines makes the selection of an appropriate cell line for the differentiation of a particular cell lineage difficult. Here, we report identification of WNT3 as a biomarker capable of predicting definitive endoderm (DE) differentiation potential of hESCs. We show that the mRNA level of WNT3 in hESCs correlates with their DE differentiation efficiency. In addition, manipulations of hESCs through WNT3 knockdown or overexpression can respectively inhibit or promote DE differentiation in a WNT3 level-dependent manner. Finally, analysis of several hESC lines based on their WNT3 expression levels allowed accurate prediction of their DE differentiation potential. Collectively, our study supports the notion that WNT3 can serve as a biomarker for predicting DE differentiation potential of hESCs.

Duke Scholars

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Published In

Stem cell reports

DOI

EISSN

2213-6711

ISSN

2213-6711

Publication Date

January 2013

Volume

1

Issue

1

Start / End Page

46 / 52

Related Subject Headings

  • Wnt3 Protein
  • RNA, Messenger
  • Induced Pluripotent Stem Cells
  • Humans
  • Endoderm
  • Embryonic Stem Cells
  • Cells, Cultured
  • Cell Lineage
  • Cell Differentiation
  • Biomarkers
 

Citation

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Jiang, W., Zhang, D., Bursac, N., & Zhang, Y. (2013). WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs. Stem Cell Reports, 1(1), 46–52. https://doi.org/10.1016/j.stemcr.2013.03.003
Jiang, Wei, Donghui Zhang, Nenad Bursac, and Yi Zhang. “WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs.Stem Cell Reports 1, no. 1 (January 2013): 46–52. https://doi.org/10.1016/j.stemcr.2013.03.003.
Jiang W, Zhang D, Bursac N, Zhang Y. WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs. Stem cell reports. 2013 Jan;1(1):46–52.
Jiang, Wei, et al. “WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs.Stem Cell Reports, vol. 1, no. 1, Jan. 2013, pp. 46–52. Epmc, doi:10.1016/j.stemcr.2013.03.003.
Jiang W, Zhang D, Bursac N, Zhang Y. WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs. Stem cell reports. 2013 Jan;1(1):46–52.
Journal cover image

Published In

Stem cell reports

DOI

EISSN

2213-6711

ISSN

2213-6711

Publication Date

January 2013

Volume

1

Issue

1

Start / End Page

46 / 52

Related Subject Headings

  • Wnt3 Protein
  • RNA, Messenger
  • Induced Pluripotent Stem Cells
  • Humans
  • Endoderm
  • Embryonic Stem Cells
  • Cells, Cultured
  • Cell Lineage
  • Cell Differentiation
  • Biomarkers