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Comparative and functional evaluation of in vitro generated to ex vivo CD8 T cells.

Publication ,  Journal Article
Dervovic, DD; Ciofani, M; Kianizad, K; Zúñiga-Pflücker, JC
Published in: J Immunol
October 1, 2012

The generation of the cytotoxic CD8 T cell response is dependent on the functional outcomes imposed by the intrathymic constraints of differentiation and self-tolerance. Although thymic function can be partly replicated in vitro using OP9-DL1 cell cultures to yield CD8 αβ TCR-bearing cells from hematopoietic progenitor cells, a comprehensive and functional assessment of entirely in vitro generated CD8 T cells derived from bone marrow hematopoietic stem cells has not been established and remains controversial. In this study, we demonstrate that a phenotypic, molecular, and functional signature of in vitro derived CD8 T cells is akin to that of ex vivo CD8 T cells, although several significant differences were also observed. Transfer of in vitro derived CD8 T cells into syngeneic and immunodeficient host mice showed no graft-versus-host response, whereas a robust homeostatic proliferation was observed, respectively. These findings, along with a diverse and broad TCR repertoire expressed by the in vitro derived CD8 T cells, allowed for the successful generation of Ag-specific T cells to be obtained from an entirely in vitro generated CD8 T cell pool. These findings support the use of Ag-specific in vitro derived effector CD8 T cells for immune reconstitution approaches, which would be amenable to further tailoring for their use against viral infections or malignancies.

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Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

October 1, 2012

Volume

189

Issue

7

Start / End Page

3411 / 3420

Location

United States

Related Subject Headings

  • Stromal Cells
  • Receptors, Antigen, T-Cell
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice, Congenic
  • Mice
  • Immunophenotyping
  • Immunology
  • Hematopoietic Stem Cells
 

Citation

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Dervovic, D. D., Ciofani, M., Kianizad, K., & Zúñiga-Pflücker, J. C. (2012). Comparative and functional evaluation of in vitro generated to ex vivo CD8 T cells. J Immunol, 189(7), 3411–3420. https://doi.org/10.4049/jimmunol.1200979
Dervovic, Dzana D., Maria Ciofani, Korosh Kianizad, and Juan Carlos Zúñiga-Pflücker. “Comparative and functional evaluation of in vitro generated to ex vivo CD8 T cells.J Immunol 189, no. 7 (October 1, 2012): 3411–20. https://doi.org/10.4049/jimmunol.1200979.
Dervovic DD, Ciofani M, Kianizad K, Zúñiga-Pflücker JC. Comparative and functional evaluation of in vitro generated to ex vivo CD8 T cells. J Immunol. 2012 Oct 1;189(7):3411–20.
Dervovic, Dzana D., et al. “Comparative and functional evaluation of in vitro generated to ex vivo CD8 T cells.J Immunol, vol. 189, no. 7, Oct. 2012, pp. 3411–20. Pubmed, doi:10.4049/jimmunol.1200979.
Dervovic DD, Ciofani M, Kianizad K, Zúñiga-Pflücker JC. Comparative and functional evaluation of in vitro generated to ex vivo CD8 T cells. J Immunol. 2012 Oct 1;189(7):3411–3420.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

October 1, 2012

Volume

189

Issue

7

Start / End Page

3411 / 3420

Location

United States

Related Subject Headings

  • Stromal Cells
  • Receptors, Antigen, T-Cell
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice, Congenic
  • Mice
  • Immunophenotyping
  • Immunology
  • Hematopoietic Stem Cells