Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density.

Published online

Journal Article

B cells expressing high affinity antigen receptors are advantaged in germinal centers (GC), perhaps by increased acquisition of antigen for presentation to follicular helper T cells and improved T-cell help. In this model for affinity-dependent selection, the density of peptide/MHCII (pMHCII) complexes on GC B cells is the primary determinant of selection. Here we show in chimeric mice populated by B cells differing only in their capacity to express MHCII (MHCII+/+ and MHCII+/-) that GC selection is insensitive to halving pMHCII density. Alone, both B cell types generate identical humoral responses; in competition, MHCII+/+ B cells are preferentially recruited to early GCs but this advantage does not persist once GCs are established. During GC responses, competing MHCII+/+ and MHCII+/- GC B cells comparably accumulate mutations and have indistinguishable rates of affinity maturation. We conclude that B-cell selection by pMHCII density is stringent in the establishment of GCs, but relaxed during GC responses.

Full Text

Duke Authors

Cited Authors

  • Yeh, C-H; Nojima, T; Kuraoka, M; Kelsoe, G

Published Date

  • March 2, 2018

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 928 -

PubMed ID

  • 29500348

Pubmed Central ID

  • 29500348

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-018-03382-x

Language

  • eng

Conference Location

  • England