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Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density.

Publication ,  Journal Article
Yeh, C-H; Nojima, T; Kuraoka, M; Kelsoe, G
Published in: Nat Commun
March 2, 2018

B cells expressing high affinity antigen receptors are advantaged in germinal centers (GC), perhaps by increased acquisition of antigen for presentation to follicular helper T cells and improved T-cell help. In this model for affinity-dependent selection, the density of peptide/MHCII (pMHCII) complexes on GC B cells is the primary determinant of selection. Here we show in chimeric mice populated by B cells differing only in their capacity to express MHCII (MHCII+/+ and MHCII+/-) that GC selection is insensitive to halving pMHCII density. Alone, both B cell types generate identical humoral responses; in competition, MHCII+/+ B cells are preferentially recruited to early GCs but this advantage does not persist once GCs are established. During GC responses, competing MHCII+/+ and MHCII+/- GC B cells comparably accumulate mutations and have indistinguishable rates of affinity maturation. We conclude that B-cell selection by pMHCII density is stringent in the establishment of GCs, but relaxed during GC responses.

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Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

March 2, 2018

Volume

9

Issue

1

Start / End Page

928

Location

England

Related Subject Headings

  • Mice, Inbred C57BL
  • Immunity, Humoral
  • Germinal Center
  • Genes, MHC Class II
  • Female
  • B-Lymphocytes
  • Animals
 

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Yeh, C.-H., Nojima, T., Kuraoka, M., & Kelsoe, G. (2018). Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun, 9(1), 928. https://doi.org/10.1038/s41467-018-03382-x
Yeh, Chen-Hao, Takuya Nojima, Masayuki Kuraoka, and Garnett Kelsoe. “Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density.Nat Commun 9, no. 1 (March 2, 2018): 928. https://doi.org/10.1038/s41467-018-03382-x.
Yeh C-H, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018 Mar 2;9(1):928.
Yeh, Chen-Hao, et al. “Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density.Nat Commun, vol. 9, no. 1, Mar. 2018, p. 928. Pubmed, doi:10.1038/s41467-018-03382-x.
Yeh C-H, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018 Mar 2;9(1):928.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

March 2, 2018

Volume

9

Issue

1

Start / End Page

928

Location

England

Related Subject Headings

  • Mice, Inbred C57BL
  • Immunity, Humoral
  • Germinal Center
  • Genes, MHC Class II
  • Female
  • B-Lymphocytes
  • Animals