Injectable tissue integrating networks from recombinant polypeptides with tunable order.

Published

Journal Article

Emergent properties of natural biomaterials result from the collective effects of nanoscale interactions among ordered and disordered domains. Here, using recombinant sequence design, we have created a set of partially ordered polypeptides to study emergent hierarchical structures by precisely encoding nanoscale order-disorder interactions. These materials, which combine the stimuli-responsiveness of disordered elastin-like polypeptides and the structural stability of polyalanine helices, are thermally responsive with tunable thermal hysteresis and the ability to reversibly form porous, viscoelastic networks above threshold temperatures. Through coarse-grain simulations, we show that hysteresis arises from physical crosslinking due to mesoscale phase separation of ordered and disordered domains. On injection of partially ordered polypeptides designed to transition at body temperature, they form stable, porous scaffolds that rapidly integrate into surrounding tissue with minimal inflammation and a high degree of vascularization. Sequence-level modulation of structural order and disorder is an untapped principle for the design of functional protein-based biomaterials.

Full Text

Duke Authors

Cited Authors

  • Roberts, S; Harmon, TS; Schaal, JL; Miao, V; Li, KJ; Hunt, A; Wen, Y; Oas, TG; Collier, JH; Pappu, RV; Chilkoti, A

Published Date

  • December 2018

Published In

Volume / Issue

  • 17 / 12

Start / End Page

  • 1154 - 1163

PubMed ID

  • 30323334

Pubmed Central ID

  • 30323334

International Standard Serial Number (ISSN)

  • 1476-1122

Digital Object Identifier (DOI)

  • 10.1038/s41563-018-0182-6

Language

  • eng

Conference Location

  • England