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Injectable tissue integrating networks from recombinant polypeptides with tunable order.

Publication ,  Journal Article
Roberts, S; Harmon, TS; Schaal, JL; Miao, V; Li, KJ; Hunt, A; Wen, Y; Oas, TG; Collier, JH; Pappu, RV; Chilkoti, A
Published in: Nat Mater
December 2018

Emergent properties of natural biomaterials result from the collective effects of nanoscale interactions among ordered and disordered domains. Here, using recombinant sequence design, we have created a set of partially ordered polypeptides to study emergent hierarchical structures by precisely encoding nanoscale order-disorder interactions. These materials, which combine the stimuli-responsiveness of disordered elastin-like polypeptides and the structural stability of polyalanine helices, are thermally responsive with tunable thermal hysteresis and the ability to reversibly form porous, viscoelastic networks above threshold temperatures. Through coarse-grain simulations, we show that hysteresis arises from physical crosslinking due to mesoscale phase separation of ordered and disordered domains. On injection of partially ordered polypeptides designed to transition at body temperature, they form stable, porous scaffolds that rapidly integrate into surrounding tissue with minimal inflammation and a high degree of vascularization. Sequence-level modulation of structural order and disorder is an untapped principle for the design of functional protein-based biomaterials.

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Published In

Nat Mater

DOI

EISSN

1476-4660

Publication Date

December 2018

Volume

17

Issue

12

Start / End Page

1154 / 1163

Location

England

Related Subject Headings

  • Viscosity
  • Temperature
  • Recombinant Proteins
  • Porosity
  • Peptides
  • Nanoscience & Nanotechnology
  • Injections
  • Elastin
  • Elasticity
 

Citation

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ICMJE
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Roberts, S., Harmon, T. S., Schaal, J. L., Miao, V., Li, K. J., Hunt, A., … Chilkoti, A. (2018). Injectable tissue integrating networks from recombinant polypeptides with tunable order. Nat Mater, 17(12), 1154–1163. https://doi.org/10.1038/s41563-018-0182-6
Roberts, Stefan, Tyler S. Harmon, Jeffrey L. Schaal, Vincent Miao, Kan Jonathan Li, Andrew Hunt, Yi Wen, et al. “Injectable tissue integrating networks from recombinant polypeptides with tunable order.Nat Mater 17, no. 12 (December 2018): 1154–63. https://doi.org/10.1038/s41563-018-0182-6.
Roberts S, Harmon TS, Schaal JL, Miao V, Li KJ, Hunt A, et al. Injectable tissue integrating networks from recombinant polypeptides with tunable order. Nat Mater. 2018 Dec;17(12):1154–63.
Roberts, Stefan, et al. “Injectable tissue integrating networks from recombinant polypeptides with tunable order.Nat Mater, vol. 17, no. 12, Dec. 2018, pp. 1154–63. Pubmed, doi:10.1038/s41563-018-0182-6.
Roberts S, Harmon TS, Schaal JL, Miao V, Li KJ, Hunt A, Wen Y, Oas TG, Collier JH, Pappu RV, Chilkoti A. Injectable tissue integrating networks from recombinant polypeptides with tunable order. Nat Mater. 2018 Dec;17(12):1154–1163.

Published In

Nat Mater

DOI

EISSN

1476-4660

Publication Date

December 2018

Volume

17

Issue

12

Start / End Page

1154 / 1163

Location

England

Related Subject Headings

  • Viscosity
  • Temperature
  • Recombinant Proteins
  • Porosity
  • Peptides
  • Nanoscience & Nanotechnology
  • Injections
  • Elastin
  • Elasticity