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Modulation of neuroinflammation and memory dysfunction using percutaneous vagus nerve stimulation in mice.

Publication ,  Journal Article
Huffman, WJ; Subramaniyan, S; Rodriguiz, RM; Wetsel, WC; Grill, WM; Terrando, N
Published in: Brain Stimul
2019

BACKGROUND: The vagus nerve is involved in regulating immunity and resolving inflammation. Current strategies aimed at modulating neuroinflammation and cognitive decline, in many cases, are limited and ineffective. OBJECTIVE: We sought to develop a minimally invasive, targeted, vagus nerve stimulation approach (pVNS), and we tested its efficacy with respect to microglial activation and amelioration of cognitive dysfunction following lipopolysaccharide (LPS) endotoxemia in mice. METHODS: We stimulated the cervical vagus nerve in mice using an ultrasound-guided needle electrode under sevoflurane anesthesia. The concentric bipolar needle electrode was percutaneously placed adjacent to the carotid sheath and stimulation was verified in real-time using bradycardia as a biomarker. Activation of vagal fibers was confirmed with immunostaining in relevant brainstem structures, including the dorsal motor nucleus and nucleus tractus solitarius. Efficacy of pVNS was evaluated following administration of LPS and analyses of changes in inflammation and behavior. RESULTS: pVNS enabled stimulation of the vagus nerve as demonstrated by changes in bradycardia and histological evaluation of c-Fos and choline acetyltransferase expression in brainstem nuclei. Following LPS administration, pVNS significantly reduced plasma levels of tumor necrosis factor-α at 3 h post-injection. pVNS prevented LPS-induced hippocampal microglial activation as analyzed by changes in Iba-1 immunoreactivity, including cell body enlargement and shortened ramifications. Cognitive dysfunction following endotoxemia was also restored by pVNS. CONCLUSION: Targeted cervical VNS using this novel percutaneous approach reduced LPS-induced systemic and brain inflammation and significantly improved cognitive responses. These results provide a novel therapeutic approach using bioelectronic medicine to modulate neuro-immune interactions that affect cognition.

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Published In

Brain Stimul

DOI

EISSN

1876-4754

Publication Date

2019

Volume

12

Issue

1

Start / End Page

19 / 29

Location

United States

Related Subject Headings

  • Vagus Nerve Stimulation
  • Solitary Nucleus
  • Neurology & Neurosurgery
  • Mice
  • Male
  • Lipopolysaccharides
  • Inflammation
  • Endotoxemia
  • Cytokines
  • Cognitive Dysfunction
 

Citation

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Chicago
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Huffman, W. J., Subramaniyan, S., Rodriguiz, R. M., Wetsel, W. C., Grill, W. M., & Terrando, N. (2019). Modulation of neuroinflammation and memory dysfunction using percutaneous vagus nerve stimulation in mice. Brain Stimul, 12(1), 19–29. https://doi.org/10.1016/j.brs.2018.10.005
Huffman, William J., Saraswathi Subramaniyan, Ramona M. Rodriguiz, William C. Wetsel, Warren M. Grill, and Niccolò Terrando. “Modulation of neuroinflammation and memory dysfunction using percutaneous vagus nerve stimulation in mice.Brain Stimul 12, no. 1 (2019): 19–29. https://doi.org/10.1016/j.brs.2018.10.005.
Huffman WJ, Subramaniyan S, Rodriguiz RM, Wetsel WC, Grill WM, Terrando N. Modulation of neuroinflammation and memory dysfunction using percutaneous vagus nerve stimulation in mice. Brain Stimul. 2019;12(1):19–29.
Huffman, William J., et al. “Modulation of neuroinflammation and memory dysfunction using percutaneous vagus nerve stimulation in mice.Brain Stimul, vol. 12, no. 1, 2019, pp. 19–29. Pubmed, doi:10.1016/j.brs.2018.10.005.
Huffman WJ, Subramaniyan S, Rodriguiz RM, Wetsel WC, Grill WM, Terrando N. Modulation of neuroinflammation and memory dysfunction using percutaneous vagus nerve stimulation in mice. Brain Stimul. 2019;12(1):19–29.
Journal cover image

Published In

Brain Stimul

DOI

EISSN

1876-4754

Publication Date

2019

Volume

12

Issue

1

Start / End Page

19 / 29

Location

United States

Related Subject Headings

  • Vagus Nerve Stimulation
  • Solitary Nucleus
  • Neurology & Neurosurgery
  • Mice
  • Male
  • Lipopolysaccharides
  • Inflammation
  • Endotoxemia
  • Cytokines
  • Cognitive Dysfunction