NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches.

Published online

Journal Article

Phytochromes initiate chloroplast biogenesis by activating genes encoding the photosynthetic apparatus, including photosynthesis-associated plastid-encoded genes (PhAPGs). PhAPGs are transcribed by a bacterial-type RNA polymerase (PEP), but how phytochromes in the nucleus activate chloroplast gene expression remains enigmatic. We report here a forward genetic screen in Arabidopsis that identified NUCLEAR CONTROL OF PEP ACTIVITY (NCP) as a necessary component of phytochrome signaling for PhAPG activation. NCP is dual-targeted to plastids and the nucleus. While nuclear NCP mediates the degradation of two repressors of chloroplast biogenesis, PIF1 and PIF3, NCP in plastids promotes the assembly of the PEP complex for PhAPG transcription. NCP and its paralog RCB are non-catalytic thioredoxin-like proteins that diverged in seed plants to adopt nonredundant functions in phytochrome signaling. These results support a model in which phytochromes control PhAPG expression through light-dependent double nuclear and plastidial switches that are linked by evolutionarily conserved and dual-localized regulatory proteins.

Full Text

Duke Authors

Cited Authors

  • Yang, EJ; Yoo, CY; Liu, J; Wang, H; Cao, J; Li, F-W; Pryer, KM; Sun, T-P; Weigel, D; Zhou, P; Chen, M

Published Date

  • June 14, 2019

Published In

Volume / Issue

  • 10 / 1

Start / End Page

  • 2630 -

PubMed ID

  • 31201314

Pubmed Central ID

  • 31201314

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-019-10517-1

Language

  • eng

Conference Location

  • England