Skip to main content

Associations of osteopontin and NT-proBNP with circulating miRNA levels in acute coronary syndrome.

Publication ,  Journal Article
Kwee, LC; Neely, ML; Grass, E; Gregory, SG; Roe, MT; Ohman, EM; Fox, KAA; White, HD; Armstrong, PW; Bowsman, LM; Haas, JV; Duffin, KL ...
Published in: Physiol Genomics
October 1, 2019

The genomic regulatory networks underlying the pathogenesis of non-ST-segment elevation acute coronary syndrome (NSTE-ACS) are incompletely understood. As intermediate traits, protein biomarkers report on underlying disease severity and prognosis in NSTE-ACS. We hypothesized that integration of dense microRNA (miRNA) profiling with biomarker measurements would highlight potential regulatory pathways that underlie the relationships between prognostic biomarkers, miRNAs, and cardiovascular phenotypes. We performed miRNA sequencing using whole blood from 186 patients from the TRILOGY-ACS trial. Seven circulating prognostic biomarkers were measured: NH2-terminal pro-B-type natriuretic peptide (NT-proBNP), high-sensitivity C-reactive protein, osteopontin (OPN), myeloperoxidase, growth differentiation factor 15, monocyte chemoattractant protein, and neopterin. We tested miRNAs for association with each biomarker with generalized linear models and controlled the false discovery rate at 0.05. Ten miRNAs, including known cardiac-related miRNAs 25-3p and 423-3p, were associated with NT-proBNP levels (min. P = 7.5 × 10-4) and 48 miRNAs, including cardiac-related miRNAs 378a-3p, 20b-5p and 320a, -b, and -d, were associated with OPN levels (min. P = 1.6 × 10-6). NT-proBNP and OPN were also associated with time to cardiovascular death, myocardial infarction (MI), or stroke in the sample. By integrating large-scale miRNA profiling with circulating biomarkers as intermediate traits, we identified associations of known cardiac-related and novel miRNAs with two prognostic biomarkers and identified potential genomic networks regulating these biomarkers. These results, highlighting plausible biological pathways connecting miRNAs with biomarkers and outcomes, may inform future studies seeking to delineate genomic pathways underlying NSTE-ACS outcomes.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Physiol Genomics

DOI

EISSN

1531-2267

Publication Date

October 1, 2019

Volume

51

Issue

10

Start / End Page

506 / 515

Location

United States

Related Subject Headings

  • Sequence Analysis, RNA
  • Risk Factors
  • Prognosis
  • Phenotype
  • Peptide Fragments
  • Osteopontin
  • Natriuretic Peptide, Brain
  • Middle Aged
  • Male
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kwee, L. C., Neely, M. L., Grass, E., Gregory, S. G., Roe, M. T., Ohman, E. M., … Shah, S. H. (2019). Associations of osteopontin and NT-proBNP with circulating miRNA levels in acute coronary syndrome. Physiol Genomics, 51(10), 506–515. https://doi.org/10.1152/physiolgenomics.00033.2019
Kwee, Lydia Coulter, Megan L. Neely, Elizabeth Grass, Simon G. Gregory, Matthew T. Roe, E Magnus Ohman, Keith A. A. Fox, et al. “Associations of osteopontin and NT-proBNP with circulating miRNA levels in acute coronary syndrome.Physiol Genomics 51, no. 10 (October 1, 2019): 506–15. https://doi.org/10.1152/physiolgenomics.00033.2019.
Kwee LC, Neely ML, Grass E, Gregory SG, Roe MT, Ohman EM, et al. Associations of osteopontin and NT-proBNP with circulating miRNA levels in acute coronary syndrome. Physiol Genomics. 2019 Oct 1;51(10):506–15.
Kwee, Lydia Coulter, et al. “Associations of osteopontin and NT-proBNP with circulating miRNA levels in acute coronary syndrome.Physiol Genomics, vol. 51, no. 10, Oct. 2019, pp. 506–15. Pubmed, doi:10.1152/physiolgenomics.00033.2019.
Kwee LC, Neely ML, Grass E, Gregory SG, Roe MT, Ohman EM, Fox KAA, White HD, Armstrong PW, Bowsman LM, Haas JV, Duffin KL, Chan MY, Shah SH. Associations of osteopontin and NT-proBNP with circulating miRNA levels in acute coronary syndrome. Physiol Genomics. 2019 Oct 1;51(10):506–515.

Published In

Physiol Genomics

DOI

EISSN

1531-2267

Publication Date

October 1, 2019

Volume

51

Issue

10

Start / End Page

506 / 515

Location

United States

Related Subject Headings

  • Sequence Analysis, RNA
  • Risk Factors
  • Prognosis
  • Phenotype
  • Peptide Fragments
  • Osteopontin
  • Natriuretic Peptide, Brain
  • Middle Aged
  • Male
  • Humans