Massive clonal expansion of medulloblastoma-specific T cells during adoptive cellular therapy.

Published online

Journal Article

In both human and murine systems, we have developed an adoptive cellular therapy platform against medulloblastoma and glioblastoma that uses dendritic cells pulsed with a tumor RNA transcriptome to expand polyclonal tumor-reactive T cells against a plurality of antigens within heterogeneous brain tumors. We demonstrate that peripheral TCR Vβ repertoire analysis after adoptive cellular therapy reveals that effective response to adoptive cellular therapy is concordant with massive in vivo expansion and persistence of tumor-specific T cell clones within the peripheral blood. In preclinical models of medulloblastoma and glioblastoma, and in a patient with relapsed medulloblastoma receiving adoptive cellular therapy, an early and massive expansion of tumor-reactive lymphocytes, coupled with prolonged persistence in the peripheral blood, is observed during effective therapeutic response to immunotherapy treatment.

Full Text

Duke Authors

Cited Authors

  • Flores, C; Wildes, T; Dean, BD; Moore, G; Drake, J; Abraham, R; Gil, J; Yegorov, O; Yang, C; Dean, J; Moneypenny, C; Shin, D; Pham, C; Krauser, J; King, J; Grant, G; Driscoll, T; Kurtzberg, J; McLendon, R; Gururangan, S; Mitchell, D

Published Date

  • November 2019

Published In

Volume / Issue

  • 5 / 11

Start / End Page

  • eaav9879 -

PubMed ID

  • 31807694

Pubmed Central ID

  • 31807694

Electronic International Standard Serial Number (EISSN)

  • 2375-2548

Digital Object Identifier (DOI)

  • 10.1126/sciadv.aav9879

Language

  • eng

Conference Location

  • United States