Isolation of Retinal Exosome Biomarkers from Blood by Targeted Immunocapture.

Published

Journal Article (Review)

The retinal pigmented epithelium (RPE) forms the outer blood-retinal barrier, provides nutrients, recycles visual pigment, and removes spent discs from the photoreceptors, among many other functions. Because of these critical roles in visual homeostasis, the RPE is a principal location of disease-associated changes in age-related macular degeneration (AMD), emphasizing its importance for study in both visual health and disease. Unfortunately, there are no early indicators of AMD or disease progression, a void that could be filled by the development of early AMD biomarkers. Exosomes are lipid bilayer membrane vesicles of nanoscale sizes that are released in a controlled fashion by cells and carry out a number of extra- and intercellular activities. In the RPE they are released from both the apical and basal sides, and each source has a unique signature/content. Exosomes released from the basolateral side of RPE cells enter the systemic circulation via the choroid and thus represent a potential source of retinal disease biomarkers in blood. Here we discuss the potential of targeted immunocapture of eye-derived exosomes and other small extracellular vesicles from blood for eye disease biomarker discovery.

Full Text

Duke Authors

Cited Authors

  • Klingeborn, M; Skiba, NP; Stamer, WD; Bowes Rickman, C

Published Date

  • 2019

Published In

Volume / Issue

  • 1185 /

Start / End Page

  • 21 - 25

PubMed ID

  • 31884583

Pubmed Central ID

  • 31884583

International Standard Serial Number (ISSN)

  • 0065-2598

Digital Object Identifier (DOI)

  • 10.1007/978-3-030-27378-1_4

Language

  • eng

Conference Location

  • United States