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RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures.

Publication ,  Journal Article
Li, D; Bradley, T; Cain, DW; Pedroza-Pacheco, I; Aggelakopoulou, M; Parks, R; Barr, M; Xia, S-M; Scearce, R; Bowman, C; Stevens, G; Newman, A ...
Published in: Immunohorizons
November 10, 2020

Rab11 recycling endosomes are involved in immunological synaptic functions, but the roles of Rab11 family-interacting protein 5 (Rab11Fip5), one of the Rab11 effectors, in the immune system remain obscure. Our previous study demonstrated that RAB11FIP5 transcripts are significantly elevated in PBMCs from HIV-1-infected individuals, making broadly HIV-1-neutralizing Abs compared with those without broadly neutralizing Abs; however, the role of Rab11FiP5 in immune functions remains unclear. In this study, a RAB11FIP5 gene knockout (RAB11FIP5 -/-) mouse model was employed to study the role of Rab11Fip5 in immune responses. RAB11FIP5 -/- mice exhibited no perturbation in lymphoid tissue cell subsets, and Rab11Fip5 was not required for serum Ab induction following HIV-1 envelope immunization, Ab transcytosis to mucosal sites, or survival after influenza challenge. However, differences were observed in multiple transcripts, including cytokine genes, in lymphocyte subsets from envelope-immunized RAB11FIP5 -/- versus control mice. These included alterations in several genes in NK cells that mirrored observations in NKs from HIV-infected humans expressing less RAB11FIP5, although Rab11Fip5 was dispensable for NK cell cytolytic activity. Notably, immunized RAB11FIP5 -/- mice had lower IL4 expression in CD4+ T follicular helper cells and showed lower TNF expression in CD8+ T cells. Likewise, TNF-α production by human CD8+ T cells correlated with PBMC RAB11FIP5 expression. These observations in RAB11FIP5 -/- mice suggest a role for Rab11Fip5 in regulating cytokine responses.

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Published In

Immunohorizons

DOI

EISSN

2573-7732

Publication Date

November 10, 2020

Volume

4

Issue

11

Start / End Page

713 / 728

Location

United States

Related Subject Headings

  • Transcriptome
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Killer Cells, Natural
  • Humans
  • HIV-1
  • HIV Infections
  • Female
  • Cytokines
 

Citation

APA
Chicago
ICMJE
MLA
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Li, D., Bradley, T., Cain, D. W., Pedroza-Pacheco, I., Aggelakopoulou, M., Parks, R., … Haynes, B. F. (2020). RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures. Immunohorizons, 4(11), 713–728. https://doi.org/10.4049/immunohorizons.2000088
Li, Dapeng, Todd Bradley, Derek W. Cain, Isabela Pedroza-Pacheco, Maria Aggelakopoulou, Robert Parks, Maggie Barr, et al. “RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures.Immunohorizons 4, no. 11 (November 10, 2020): 713–28. https://doi.org/10.4049/immunohorizons.2000088.
Li D, Bradley T, Cain DW, Pedroza-Pacheco I, Aggelakopoulou M, Parks R, et al. RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures. Immunohorizons. 2020 Nov 10;4(11):713–28.
Li, Dapeng, et al. “RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures.Immunohorizons, vol. 4, no. 11, Nov. 2020, pp. 713–28. Pubmed, doi:10.4049/immunohorizons.2000088.
Li D, Bradley T, Cain DW, Pedroza-Pacheco I, Aggelakopoulou M, Parks R, Barr M, Xia S-M, Scearce R, Bowman C, Stevens G, Newman A, Hora B, Chen Y, Riebe K, Wang Y, Sempowski G, Saunders KO, Borrow P, Haynes BF. RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures. Immunohorizons. 2020 Nov 10;4(11):713–728.

Published In

Immunohorizons

DOI

EISSN

2573-7732

Publication Date

November 10, 2020

Volume

4

Issue

11

Start / End Page

713 / 728

Location

United States

Related Subject Headings

  • Transcriptome
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Killer Cells, Natural
  • Humans
  • HIV-1
  • HIV Infections
  • Female
  • Cytokines