Essential roles of S-nitrosothiols in vascular homeostasis and endotoxic shock.

Journal Article (Journal Article)

The current perspective of NO biology is formulated predominantly from studies of NO synthesis. The role of S-nitrosothiol (SNO) formation and turnover in governing NO-related bioactivity remains uncertain. We generated mice with a targeted gene deletion of S-nitrosoglutathione reductase (GSNOR), and show that they exhibit substantial increases in whole-cell S-nitrosylation, tissue damage, and mortality following endotoxic or bacterial challenge. Further, GSNOR(-/-) mice have increased basal levels of SNOs in red blood cells and are hypotensive under anesthesia. Thus, SNOs regulate innate immune and vascular function, and are cleared actively to ameliorate nitrosative stress. Nitrosylation of cysteine thiols is a critical mechanism of NO function in both health and disease.

Full Text

Duke Authors

Cited Authors

  • Liu, L; Yan, Y; Zeng, M; Zhang, J; Hanes, MA; Ahearn, G; McMahon, TJ; Dickfeld, T; Marshall, HE; Que, LG; Stamler, JS

Published Date

  • February 20, 2004

Published In

Volume / Issue

  • 116 / 4

Start / End Page

  • 617 - 628

PubMed ID

  • 14980227

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(04)00131-x


  • eng

Conference Location

  • United States