Loretta Georgina Que | Scholars@Duke

Loretta Georgina Que
Associate Professor of Medicine

My research interests focus on studying the role of nitric oxide and related enzymes in the pathogenesis of lung disease, specifically that caused by nitrosative/oxidative stress. Proposed studies are performed in cell culture and applied to animal models of disease, then examined in human disease where relevant. It is our hope that by better understanding the role of NO and reactive nitrogen species in mediating inflammation, and regulating cell signaling, that we will not only help to unravel the basic mechanisms of NO related lung disease, but also provide a rationale for targeted therapeutic use of NO.

Key words: nitrosative defense, lung injury, nitric oxide

Current Appointments & Affiliations

Associate Professor of Medicine, Medicine, Pulmonary, Allergy, and Critical Care Medicine, Medicine 2011

Contact Information

279 MSRB1, Durham, NC 27710
Duke Box 3132, Durham, NC 27710
loretta.que@duke.edu (919) 681-8551

Background
Education, Training, & Certifications
- Pulmonary Fellow, Medicine, Duke University 1993 - 1996
- Clinical Instructor, Medicine, Yale University 1992 - 1993
- Medical Resident, Medicine, Yale University 1989 - 1992
- M.D., University of Chicago 1989
Duke Appointment History
- Assistant Professor of Medicine, Medicine, Pulmonary, Allergy, and Critical Care Medicine, Medicine 2001 - 2010
- Associate in the Department of Medicine, Medicine, Pulmonary, Allergy, and Critical Care Medicine, Medicine 1996 - 2001
Research
Selected Grants
- A Longitudinal Prospective Observational Study of the Characteristics, Treatment Patterns and Health Outcomes of Individuals with Severe Asthma in the United States awarded by AstraZeneca Pharmaceuticals, LP 2018 - 2023
- CXCL10/CXCR3 regulation of ozone-induced epithelial permeability awarded by National Institutes of Health 2017 - 2022
- Research Training in Allergy and Clinical Immunology awarded by National Institutes of Health 2000 - 2021
- Mechanisms that Direct Airway Remodeling in Obese Asthma awarded by National Institutes of Health 2017 - 2021
- Early Detection of Changes in Pulmonary Gas Exchange by Hyperpolarized Xe MRI awarded by National Institutes of Health 2011 - 2021
- Interdisciplinary Training Program in Lung Disease awarded by National Institutes of Health 2009 - 2020
- Trial of Roflumilast in Asthma Management (TRIM) awarded by American Lung Association 2018 - 2019
- Pilot: Zinc Acetate to Treat Chronic Refractory Cough (ZICO) awarded by American Lung Association 2017 - 2019
- ALA-ACRC Network awarded by American Lung Association 2016 - 2019
- VTEU Task Order 16-0058 - Task B awarded by National Institutes of Health 2017 - 2019
- VTEU Task Order 16-0058 - Task C awarded by National Institutes of Health 2017 - 2019
- VTEU Task Order 16-0058.B1C1D1.0076 - Task D awarded by National Institutes of Health 2017 - 2019
- Modulation of Asthma by GSNO-based Therapies awarded by National Institutes of Health 2013 - 2018
- Diesel-induced Alterations of Influenza Infectivity awarded by University of North Carolina - Chapel Hill 2015 - 2017
- Surfactant Protein-A regulation of eosinophil apoptosis in asthma awarded by University of Arizona 2015 - 2017
- HIV and Asthma in the Post- ART Era awarded by National Institutes of Health 2014 - 2016
- The Duke Multidisciplinary Training Program in Pediatric Lung Disease awarded by National Institutes of Health 2010 - 2016
- Clinical Center Core awarded by University of Arizona 2009 - 2016
- Asthma Clinical Research Center of the American Lung Association awarded by American Lung Association 2009 - 2016
- I2PP2A and Asthma awarded by Cognosci, Inc. 2014 - 2015
- Diesel-induced Alterations of Influenza Infectivity awarded by University of North Carolina - Chapel Hill 2013 - 2014
- GSNO Reductase, S-Nitrosothiols, and Asthma awarded by National Institutes of Health 2007 - 2013
- Genetic regulation of O3-induced inflammation in humans awarded by National Institutes of Health 2000 - 2007
- Modulation of NO with Pulmonary Gene Transfer awarded by National Institutes of Health 2000 - 2006
Publications & Artistic Works
Selected Publications
- Journal Articles
  - Tang, M, Henderson, RJ, Holbrook, JT, Que, LG, Mathews, AM, Wise, RA, Dixon, AE, Peters, SP, Rogers, L, Smith, LJ, Teague, WG, and Lang, JE. "Does Obesity Increase Respiratory Tract Infections in Patients with Asthma?." The Journal of Allergy and Clinical Immunology. in Practice (October 9, 2018). Full Text
  - Que, LG, Yang, Z, Lugogo, NL, Katial, RK, Shoemaker, SA, Troha, JM, Rodman, DM, Tighe, RM, and Kraft, M. "Effect of the S-nitrosoglutathione reductase inhibitor N6022 on bronchial hyperreactivity in asthma." Immunity, Inflammation and Disease 6, no. 2 (June 2018): 322-331. Full Text
  - Paul, DW, Neely, NB, Clement, M, Riley, I, Al-Hegelan, M, Phelan, M, Kraft, M, Murdoch, DM, Lucas, J, Bartlett, J, McKellar, M, and Que, LG. "Development and validation of an electronic medical record (EMR)-based computed phenotype of HIV-1 infection." Journal of the American Medical Informatics Association : Jamia 25, no. 2 (February 2018): 150-157. Full Text
  - Riley, IL, Boulware, LE, Sun, J-L, Chiswell, K, Que, LG, Kraft, M, Todd, JL, Palmer, SM, and Anderson, ML. "Factors associated with reporting results for pulmonary clinical trials in ClinicalTrials.gov." Clinical Trials (London, England) 15, no. 1 (February 2018): 87-94. Full Text
  - Paul, DW, Lagat, DK, MacIntyre, N, Egger, JR, Murdoch, DM, Que, LG, and Kussin, PS. "Validation of spirometry prediction equations in western Kenya." The International Journal of Tuberculosis and Lung Disease 22, no. 1 (January 2018): 112-118. Full Text
  - Suwanpradid, J, Shih, M, Pontius, L, Yang, B, Birukova, A, Guttman-Yassky, E, Corcoran, DL, Que, LG, Tighe, RM, and MacLeod, AS. "Arginase1 Deficiency in Monocytes/Macrophages Upregulates Inducible Nitric Oxide Synthase To Promote Cutaneous Contact Hypersensitivity." Journal of Immunology (Baltimore, Md. : 1950) 199, no. 5 (September 2017): 1827-1834. Full Text
  - Durack, J, Lynch, SV, Nariya, S, Bhakta, NR, Beigelman, A, Castro, M, Dyer, A-M, Israel, E, Kraft, M, Martin, RJ, Mauger, DT, Rosenberg, SR, Sharp-King, T, White, SR, Woodruff, PG, Avila, PC, Denlinger, LC, Holguin, F, Lazarus, SC, Lugogo, N, Moore, WC, Peters, SP, Que, L, Smith, LJ, Sorkness, CA, Wechsler, ME, Wenzel, SE, Boushey, HA, Huang, YJ, National Heart, and Lung and Blood Institute's “AsthmaNet”, . "Features of the bronchial bacterial microbiome associated with atopy, asthma, and responsiveness to inhaled corticosteroid treatment." The Journal of Allergy and Clinical Immunology 140, no. 1 (July 2017): 63-75. Full Text
  - Winnica, D, Que, LG, Baffi, C, Grasemann, H, Fiedler, K, Yang, Z, Etling, E, Wasil, K, Wenzel, SE, Freeman, B, and Holguin, F. "l-citrulline prevents asymmetric dimethylarginine-mediated reductions in nitric oxide and nitrosative stress in primary human airway epithelial cells." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology 47, no. 2 (February 2017): 190-199. Full Text
  - He, M, Driehuys, B, Que, LG, and Huang, Y-CT. "Using Hyperpolarized 129Xe MRI to Quantify the Pulmonary Ventilation Distribution." Academic Radiology 23, no. 12 (December 2016): 1521-1531. Full Text
  - Ray, S, Chiba, N, Yao, C, Guan, X, McConnell, AM, Brockway, B, Que, L, McQualter, JL, and Stripp, BR. "Rare SOX2+ Airway Progenitor Cells Generate KRT5+ Cells that Repopulate Damaged Alveolar Parenchyma following Influenza Virus Infection." Stem Cell Reports 7, no. 5 (November 2016): 817-825. Full Text
  - Baffi, CW, Wood, L, Winnica, D, Strollo, PJ, Gladwin, MT, Que, LG, and Holguin, F. "Metabolic Syndrome and the Lung." Chest 149, no. 6 (June 2016): 1525-1534. (Review) Full Text
  - Ingram, JL, Slade, D, Church, TD, Francisco, D, Heck, K, Sigmon, RW, Ghio, M, Murillo, A, Firszt, R, Lugogo, NL, Que, L, Sunday, ME, and Kraft, M. "Role of Matrix Metalloproteinases-1 and -2 in Interleukin-13-Suppressed Elastin in Airway Fibroblasts in Asthma." American Journal of Respiratory Cell and Molecular Biology 54, no. 1 (January 2016): 41-50. Full Text
  - Yu, Y-RA, O'Koren, EG, Hotten, DF, Kan, MJ, Kopin, D, Nelson, ER, Que, L, and Gunn, MD. "A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues." Plos One 11, no. 3 (January 2016): e0150606-null. Full Text
  - Mehta, K, Soderblom, EJ, Thompson, JW, Que, LG, Marshall, HE, Moseley, MA, and Foster, MW. "Targeted proteomics for unraveling the post-translational regulation of human nitric oxide synthase 2." Nitric Oxide 42C (November 15, 2014): 132-. Full Text Link to Item
  - Ledford, JG, Addison, KJ, Foster, MW, and Que, LG. "Eosinophil-associated lung diseases. A cry for surfactant proteins A and D help?." American Journal of Respiratory Cell and Molecular Biology 51, no. 5 (November 2014): 604-614. (Review) Full Text
  - Castro, M, King, TS, Kunselman, SJ, Cabana, MD, Denlinger, L, Holguin, F, Kazani, SD, Moore, WC, Moy, J, Sorkness, CA, Avila, P, Bacharier, LB, Bleecker, E, Boushey, HA, Chmiel, J, Fitzpatrick, AM, Gentile, D, Hundal, M, Israel, E, Kraft, M, Krishnan, JA, LaForce, C, Lazarus, SC, Lemanske, R, Lugogo, N, Martin, RJ, Mauger, DT, Naureckas, E, Peters, SP, Phipatanakul, W, Que, LG, Sheshadri, A, Smith, L, Solway, J, Sullivan-Vedder, L, Sumino, K, Wechsler, ME, Wenzel, S, White, SR, Sutherland, ER, National Heart, Lung, and and Blood Institute’s AsthmaNet, . "Effect of vitamin D3 on asthma treatment failures in adults with symptomatic asthma and lower vitamin D levels: the VIDA randomized clinical trial." Jama 311, no. 20 (May 2014): 2083-2091. Full Text
  - Catania, J, Que, LG, Govert, JA, Hollingsworth, JW, and Wolfe, CR. "High intensive care unit admission rate for 2013-2014 influenza is associated with a low rate of vaccination." American Journal of Respiratory and Critical Care Medicine 189, no. 4 (February 2014): 485-487. (Letter) Full Text
  - Foster, MW, Thompson, JW, Que, LG, Yang, IV, Schwartz, DA, Moseley, MA, and Marshall, HE. "Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure." J Proteome Res 12, no. 5 (May 3, 2013): 2194-2205. Full Text Link to Item
  - Foster, MW, Yang, Z, Gooden, DM, Thompson, JW, Ball, CH, Turner, ME, Hou, Y, Pi, J, Moseley, MA, and Que, LG. "Proteomic characterization of the cellular response to nitrosative stress mediated by s-nitrosoglutathione reductase inhibition." J Proteome Res 11, no. 4 (April 6, 2012): 2480-2491. Full Text Link to Item
  - Wang, Y, Zhu, Z, Church, TD, Lugogo, NL, Que, LG, Francisco, D, Ingram, JL, Huggins, M, Beaver, DM, Wright, JR, and Kraft, M. "SHP-1 as a critical regulator of Mycoplasma pneumoniae-induced inflammation in human asthmatic airway epithelial cells." J Immunol 188, no. 7 (April 1, 2012): 3371-3381. Full Text Link to Item
  - Yang, IV, Tomfohr, J, Singh, J, Foss, CM, Marshall, HE, Que, LG, McElvania-Tekippe, E, Florence, S, Sundy, JS, and Schwartz, DA. "The clinical and environmental determinants of airway transcriptional profiles in allergic asthma." American Journal of Respiratory and Critical Care Medicine 185, no. 6 (March 2012): 620-627. Full Text
  - Que, LG, and Janssen-Heininger, Y. "Biochemistry of asthma." Biochimica Et Biophysica Acta 1810, no. 11 (November 2011): 1007-null. Full Text
  - Que, LG, Stiles, JV, Sundy, JS, and Foster, WM. "Pulmonary function, bronchial reactivity, and epithelial permeability are response phenotypes to ozone and develop differentially in healthy humans." Journal of Applied Physiology (Bethesda, Md. : 1985) 111, no. 3 (September 2011): 679-687. Full Text
  - Norfolk, SG, Hollingsworth, CL, Wolfe, CR, Govert, JA, Que, LG, Cheifetz, IM, and Hollingsworth, JW. "Rescue therapy in adult and pediatric patients with pH1N1 influenza infection: a tertiary center intensive care unit experience from April to October 2009." Critical Care Medicine 38, no. 11 (November 2010): 2103-2107. Full Text
  - Huang, Y-CT, Schmitt, M, Yang, Z, Que, LG, Stewart, JC, Frampton, MW, and Devlin, RB. "Gene expression profile in circulating mononuclear cells after exposure to ultrafine carbon particles." Inhal Toxicol 22, no. 10 (August 2010): 835-846. Full Text Link to Item
  - Choudhry, S, Que, LG, Yang, Z, Liu, L, Eng, C, Kim, SO, Kumar, G, Thyne, S, Chapela, R, Rodriguez-Santana, JR, Rodriguez-Cintron, W, Avila, PC, Stamler, JS, and Burchard, EG. "GSNO reductase and beta2-adrenergic receptor gene-gene interaction: bronchodilator responsiveness to albuterol." Pharmacogenetics and Genomics 20, no. 6 (June 2010): 351-358. Full Text
  - Wahidi, MM, Silvestri, GA, Coakley, RD, Ferguson, JS, Shepherd, RW, Moses, L, Conforti, J, Que, LG, Anstrom, KJ, McGuire, F, Colt, H, and Downie, GH. "A prospective multicenter study of competency metrics and educational interventions in the learning of bronchoscopy among new pulmonary fellows." Chest 137, no. 5 (May 2010): 1040-1049. Full Text
  - Que, LG, Yang, Z, Stamler, JS, Lugogo, NL, and Kraft, M. "S-nitrosoglutathione reductase: an important regulator in human asthma." American Journal of Respiratory and Critical Care Medicine 180, no. 3 (August 2009): 226-231. Full Text
  - Chudgar, SM, Cox, CE, Que, LG, Andolsek, K, Knudsen, NW, and Clay, AS. "Current teaching and evaluation methods in critical care medicine: has the Accreditation Council for Graduate Medical Education affected how we practice and teach in the intensive care unit?." Crit Care Med 37, no. 1 (January 2009): 49-60. Full Text Link to Item
  - Lugogo, NL, Ginsburg, GS, and Que, LG. "Genetic profiling and tailored therapy in asthma: are we there yet?." Curr Opin Mol Ther 9, no. 6 (December 2007): 528-537. (Review) Link to Item
  - Whalen, EJ, Foster, MW, Matsumoto, A, Ozawa, K, Violin, JD, Que, LG, Nelson, CD, Benhar, M, Keys, JR, Rockman, HA, Koch, WJ, Daaka, Y, Lefkowitz, RJ, and Stamler, JS. "Regulation of beta-adrenergic receptor signaling by S-nitrosylation of G-protein-coupled receptor kinase 2." Cell 129, no. 3 (May 4, 2007): 511-522. Full Text Link to Item
  - Clay, AS, Que, L, Petrusa, ER, Sebastian, M, and Govert, J. "Debriefing in the intensive care unit: a feedback tool to facilitate bedside teaching." Crit Care Med 35, no. 3 (March 2007): 738-754. Full Text Link to Item
  - Clay, AS, Que, LG, Govert, JA, and Andolsek, KM. ""A rose by any other name"? Toward a common terminology in simulation education and assessment [6]." Critical Care Medicine 35, no. 9 (2007): 2238--. Full Text
  - Que, LG, Liu, L, Yan, Y, Whitehead, GS, Gavett, SH, Schwartz, DA, and Stamler, JS. "Protection from experimental asthma by an endogenous bronchodilator." Science (New York, N.Y.) 308, no. 5728 (June 2005): 1618-1621. Full Text
  - Liu, L, Yan, Y, Zeng, M, Zhang, J, Hanes, MA, Ahearn, G, McMahon, TJ, Dickfeld, T, Marshall, HE, Que, LG, and Stamler, JS. "Essential roles of S-nitrosothiols in vascular homeostasis and endotoxic shock." Cell 116, no. 4 (February 2004): 617-628. Full Text
  - Yu, Q, Que, LG, and Rockey, DC. "Adenovirus-mediated gene transfer to nonparenchymal cells in normal and injured liver." American Journal of Physiology. Gastrointestinal and Liver Physiology 282, no. 3 (March 2002): G565-G572. Full Text
  - Que, LG, George, SE, Gotoh, T, Mori, M, and Huang, Y-CT. "Effects of arginase isoforms on NO Production by nNOS." Nitric Oxide : Biology and Chemistry 6, no. 1 (February 2002): 1-8. Full Text
  - Yu, Q, Que, LG, and Rockey, DC. "Adenovirus-mediated gene transfer to nonparenchymal cells in normal and injured liver." American Journal of Physiology - Gastrointestinal and Liver Physiology 282, no. 3 45-3 (2002): G565-G572.
  - Liu, L, Hausladen, A, Zeng, M, Que, L, Heitman, J, and Stamler, JS. "A metabolic enzyme for S-nitrosothiol conserved from bacteria to humans." Nature 410, no. 6827 (March 2001): 490-494. Full Text
  - Liu, L, Hausladen, A, Zeng, M, Que, L, Heitman, J, Stamler, JS, and Steverding, D. "Nitrosative stress: protection by glutathione-dependent formaldehyde dehydrogenase." Redox Rep 6, no. 4 (2001): 209-210. Full Text Link to Item
  - Que, LG, and Tony Huang, YC. "Pharmacologic adjuncts during mechanical ventilation." Semin Respir Crit Care Med 21, no. 3 (2000): 223-232. Full Text Link to Item
  - Lau, CL, Bentley, RC, Gockerman, JP, Que, LG, and D'Amico, TA. "Malignant melanoma presenting as a mediastinal mass." Ann Thorac Surg 67, no. 3 (March 1999): 851-852. Link to Item
  - Que, LG. "Ask the expert." Asthma Magazine 4, no. 5 (1999): 26--. Full Text
  - Carraway, MS, Welty-Wolf, KE, Kantrow, SP, Huang, YCT, Simonson, SG, Que, LG, Kishimoto, TK, and Piantadosi, CA. "Antibody to E- and L-selectin does not prevent lung injury or mortality in septic baboons." American Journal of Respiratory and Critical Care Medicine 157, no. 3 PART I (December 1, 1998): 938-949.
  - Que, LG, Kantrow, SP, Jenkinson, CP, Piantadosi, CA, and Huang, YC. "Induction of arginase isoforms in the lung during hyperoxia." The American Journal of Physiology 275, no. 1 Pt 1 (July 1998): L96-102.
  - Que, LG, Kantrow, SP, Jenkinson, CP, Piantadosi, CA, and Huang, YCT. "Induction of arginase isoforms in the lung during hyperoxia." American Journal of Physiology Lung Cellular and Molecular Physiology 275, no. 1 19-1 (July 1, 1998).
  - Que, LG, Kang, BH, Huang, YC, Piantadosi, CA, and Chang, LY. "Anti-intercellular adhesion molecule-1 antibody and intercellular adhesion molecule-1 gene deficiency do not prevent pulmonary neutrophil recruitment in polymicrobial sepsis." Shock 9, no. 4 (April 1998): 304-309. Link to Item
  - Carraway, MS, Welty-Wolf, KE, Kantrow, SP, Huang, YC, Simonson, SG, Que, LG, Kishimoto, TK, and Piantadosi, CA. "Antibody to E- and L-selectin does not prevent lung injury or mortality in septic baboons." Am J Respir Crit Care Med 157, no. 3 Pt 1 (March 1998): 938-949. Full Text Link to Item

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Education, Training, & Certifications

Duke Appointment History

Selected Grants

Selected Publications

Journal Articles