Protease-activated receptor-2 signaling triggers dendritic cell development.

Journal Article (Journal Article)

Dendritic cells (DC) are potent antigen-presenting cells that govern the effector cell responses of the immune system. DC are thought to continuously develop from circulating progenitors in a process that is accelerated by inflammatory stimuli. However, the physiological signals that regulate the development of DC from precursor cells have not been well defined. Here we show that a serine protease acting via protease-activated receptor-2 (PAR-2) stimulates the development of DC from bone marrow progenitor cells cultured in granulocyte-macrophage colony-stimulating factor and IL-4. DC fail to develop in bone marrow cultures treated with soy bean trypsin inhibitor, a serine protease inhibitor, but this inhibition is overcome by a PAR-2 agonist peptide. DC do not spontaneously develop from the bone marrow of PAR-2-deficient mice, but can be stimulated to do so by inflammatory mediators. These results suggest that endogenous serine proteases stimulate DC development in vitro. Thus, serine proteases may help trigger adaptive immune responses in vivo.

Full Text

Duke Authors

Cited Authors

  • Fields, RC; Schoenecker, JG; Hart, JP; Hoffman, MR; Pizzo, SV; Lawson, JH

Published Date

  • June 2003

Published In

Volume / Issue

  • 162 / 6

Start / End Page

  • 1817 - 1822

PubMed ID

  • 12759239

Pubmed Central ID

  • PMC1868121

International Standard Serial Number (ISSN)

  • 0002-9440

Digital Object Identifier (DOI)

  • 10.1016/S0002-9440(10)64316-7


  • eng

Conference Location

  • United States