Regulation of HIF-1alpha stability through S-nitrosylation.
Journal Article (Journal Article)
Hypoxia-inducible factor 1 (HIF-1) is a master transcriptional factor. Under normal oxygen tension, HIF-1 activity is usually suppressed due to the rapid, oxygen-dependent degradation of one of its two subunits, HIF-1alpha. Here we report that normoxic HIF-1 activity can be upregulated through NO-mediated S-nitrosylation and stabilization of HIF-1alpha. In murine tumors, exposure to ionizing radiation stimulated the generation of NO in tumor-associated macrophages. As a result, the HIF-1alpha protein is S-nitrosylated at Cys533 (through "biotin switch" assay) in the oxygen-dependent degradation domain, which prevents its destruction. Importantly, this mechanism appears to be independent of the prolylhydroxylase-based pathway that is involved in oxygen-dependent regulation of HIF-1alpha. Selective disruption of this S-nitrosylation significantly attenuated both radiation-induced and macrophage-induced activation of HIF-1alpha. This interaction between NO and HIF-1 sheds new light on their involvement in tumor response to treatment as well as mammalian inflammation process in general.
Full Text
Duke Authors
Cited Authors
- Li, F; Sonveaux, P; Rabbani, ZN; Liu, S; Yan, B; Huang, Q; Vujaskovic, Z; Dewhirst, MW; Li, C-Y
Published Date
- April 13, 2007
Published In
Volume / Issue
- 26 / 1
Start / End Page
- 63 - 74
PubMed ID
- 17434127
Pubmed Central ID
- PMC2905600
International Standard Serial Number (ISSN)
- 1097-2765
Digital Object Identifier (DOI)
- 10.1016/j.molcel.2007.02.024
Language
- eng
Conference Location
- United States