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Inducible expression of the alpha2-macroglobulin signaling receptor in response to antigenic stimulation: a study of second messenger generation.

Publication ,  Journal Article
Bhattacharjee, G; Misra, UK; Gawdi, G; Cianciolo, G; Pizzo, SV
Published in: J Cell Biochem
2001

Thioglycollate (TG)-elicited murine, peritoneal macrophages express two receptors for activated forms of the proteinase inhibitor alpha2-macroglobulin (alpha2M*)--namely, the low density lipoprotein receptor-related protein (LRP) and the alpha2M signaling receptor (alpha2MSR). We now report that resident peritoneal macrophages express only 400+/-50 alpha2MSR receptors/cell compared to 5000+/-500 receptor/TG-elicited macrophage. By contrast, LRP expression is only 2-2.5-fold greater on elicited cells. The low level of alpha2MSR expression by resident cells is insufficient to trigger signal transduction in contrast to TG-elicited cells which when exposed to alpha2M* demonstrate a rapid rise in inositol 1,4,5-trisphosphate and a concomitant increase in cytosolic free Ca2+. We then studied a variety of preparations injected subcutaneously for their ability to upregulate alpha2MSR. Macroaggregated bovine serum albumin (macroBSA) injection upregulated alpha2MSR and triggered signaling responses by splenic macrophages. Nonaggregated BSA injection alone or in the presence of alum, by contrast, did not alter alpha2MSR expression. Recombivax (hepatitis B antigen adsorbed to alum) injection also upregulated alpha2MSR on splenic macrophages while the alum carrier had no effect. We conclude that macrophage alpha2M* receptors are inducible and their expression may be regulated, in part, by potential antigens.

Duke Scholars

Published In

J Cell Biochem

DOI

ISSN

0730-2312

Publication Date

2001

Volume

82

Issue

2

Start / End Page

260 / 270

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Vaccines, Synthetic
  • Thioglycolates
  • Spleen
  • Specific Pathogen-Free Organisms
  • Serum Albumin, Bovine
  • Second Messenger Systems
  • Peritonitis
  • Mice, Inbred C57BL
  • Mice
 

Citation

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Bhattacharjee, G., Misra, U. K., Gawdi, G., Cianciolo, G., & Pizzo, S. V. (2001). Inducible expression of the alpha2-macroglobulin signaling receptor in response to antigenic stimulation: a study of second messenger generation. J Cell Biochem, 82(2), 260–270. https://doi.org/10.1002/jcb.1152
Bhattacharjee, G., U. K. Misra, G. Gawdi, G. Cianciolo, and S. V. Pizzo. “Inducible expression of the alpha2-macroglobulin signaling receptor in response to antigenic stimulation: a study of second messenger generation.J Cell Biochem 82, no. 2 (2001): 260–70. https://doi.org/10.1002/jcb.1152.
Bhattacharjee, G., et al. “Inducible expression of the alpha2-macroglobulin signaling receptor in response to antigenic stimulation: a study of second messenger generation.J Cell Biochem, vol. 82, no. 2, 2001, pp. 260–70. Pubmed, doi:10.1002/jcb.1152.
Bhattacharjee G, Misra UK, Gawdi G, Cianciolo G, Pizzo SV. Inducible expression of the alpha2-macroglobulin signaling receptor in response to antigenic stimulation: a study of second messenger generation. J Cell Biochem. 2001;82(2):260–270.
Journal cover image

Published In

J Cell Biochem

DOI

ISSN

0730-2312

Publication Date

2001

Volume

82

Issue

2

Start / End Page

260 / 270

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Vaccines, Synthetic
  • Thioglycolates
  • Spleen
  • Specific Pathogen-Free Organisms
  • Serum Albumin, Bovine
  • Second Messenger Systems
  • Peritonitis
  • Mice, Inbred C57BL
  • Mice