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Artificial surfactant attenuates hyperoxic lung injury in primates. II. Morphometric analysis.

Publication ,  Journal Article
Piantadosi, CA; Fracica, PJ; Duhaylongsod, FG; Huang, YC; Welty-Wolf, KE; Crapo, JD; Young, SL
Published in: J Appl Physiol (1985)
May 1995

Diffuse lung injury from hyperoxia is accompanied by low compliance and hypoxemia with disruption of endothelial and alveolar epithelial cell layers. Because both function and content of surfactant in diffuse lung injury decrease in animals and in humans, changes in the extent of injury during continuous hyperoxia were evaluated after treatments with a protein-free surfactant in primates. Ten baboons were ventilated with 100% O2 for 96 h and five were intermittently given an aerosol of an artificial surfactant (Exosurf). Physiological and biochemical measurements of the effects of the surfactant treatment are presented in a companion paper (Y.-C. T. Huang, A. C. Sane, S. G. Simonson, T. A. Fawcett, R. E. Moon, P. J. Fracica, M. G. Menache, C. A. Piantadosi, and S. L. Young. J. Appl. Physiol. 78: 1823-1829, 1995.) After O2 exposures, lungs were fixed and processed for electron microscopy. The cellular responses to O2 included epithelial and endothelial cell injuries, interstitial edema, and inflammation. Morphometry was used to quantitate changes in lungs of animals treated with the artificial surfactant during O2 exposure and to compare them with the untreated animals. The surfactant decreased neutrophil accumulation, increased fibroblast proliferation, and decreased changes in the volume of type I epithelial cells. Surfactant-treated animals also demonstrated better preservation of endothelial cell integrity. These responses indicate ameliorating effects of the surfactant on the pulmonary response to hyperoxia, including protection against epithelial and endothelial cell destruction. Significant interstitial inflammation and fibroblast proliferation remained, however, in surfactant-treated lungs exposed to continuous hyperoxia.

Duke Scholars

Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

May 1995

Volume

78

Issue

5

Start / End Page

1823 / 1831

Location

United States

Related Subject Headings

  • Pulmonary Surfactants
  • Pulmonary Alveoli
  • Polyethylene Glycols
  • Physiology
  • Phosphorylcholine
  • Papio
  • Oxygen
  • Microscopy, Electron
  • Male
  • Lung Diseases
 

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Piantadosi, C. A., Fracica, P. J., Duhaylongsod, F. G., Huang, Y. C., Welty-Wolf, K. E., Crapo, J. D., & Young, S. L. (1995). Artificial surfactant attenuates hyperoxic lung injury in primates. II. Morphometric analysis. J Appl Physiol (1985), 78(5), 1823–1831. https://doi.org/10.1152/jappl.1995.78.5.1823
Piantadosi, C. A., P. J. Fracica, F. G. Duhaylongsod, Y. C. Huang, K. E. Welty-Wolf, J. D. Crapo, and S. L. Young. “Artificial surfactant attenuates hyperoxic lung injury in primates. II. Morphometric analysis.J Appl Physiol (1985) 78, no. 5 (May 1995): 1823–31. https://doi.org/10.1152/jappl.1995.78.5.1823.
Piantadosi CA, Fracica PJ, Duhaylongsod FG, Huang YC, Welty-Wolf KE, Crapo JD, et al. Artificial surfactant attenuates hyperoxic lung injury in primates. II. Morphometric analysis. J Appl Physiol (1985). 1995 May;78(5):1823–31.
Piantadosi, C. A., et al. “Artificial surfactant attenuates hyperoxic lung injury in primates. II. Morphometric analysis.J Appl Physiol (1985), vol. 78, no. 5, May 1995, pp. 1823–31. Pubmed, doi:10.1152/jappl.1995.78.5.1823.
Piantadosi CA, Fracica PJ, Duhaylongsod FG, Huang YC, Welty-Wolf KE, Crapo JD, Young SL. Artificial surfactant attenuates hyperoxic lung injury in primates. II. Morphometric analysis. J Appl Physiol (1985). 1995 May;78(5):1823–1831.

Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

May 1995

Volume

78

Issue

5

Start / End Page

1823 / 1831

Location

United States

Related Subject Headings

  • Pulmonary Surfactants
  • Pulmonary Alveoli
  • Polyethylene Glycols
  • Physiology
  • Phosphorylcholine
  • Papio
  • Oxygen
  • Microscopy, Electron
  • Male
  • Lung Diseases