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Hypoxia in melanoma: using optical spectroscopy and EF5 to assess tumor oxygenation before and during regional chemotherapy for melanoma.

Publication ,  Journal Article
Speicher, PJ; Beasley, GM; Jiang, B; Lidsky, ME; Palmer, GM; Scarbrough, PM; Mosca, PJ; Dewhirst, MW; Tyler, DS
Published in: Ann Surg Oncol
May 2014

BACKGROUND: There is increasing evidence that tumor hypoxia plays a significant role in the chemoresistance of melanoma, but to our knowledge, real-time tumor oxygenation during isolated limb infusion (ILI) has not been studied. We sought to demonstrate the feasibility of measuring real-time alterations in tissue oxygenation. METHODS: Consecutive patients with histologically confirmed in-transit melanoma were enrolled onto a prospective single-arm pilot study and administered the hypoxia marker drug EF5. All patients were treated with ILI. Optical spectroscopy readings were obtained at three locations: two discrete target lesions and one normal skin control. Measurements were taken at 11 predefined time points during ILI. RESULTS: A total of six patients were enrolled onto this pilot study. Intratumor and normal skin optical spectroscopy readings were found to have discrete inflection points throughout the duration of therapy, corresponding with established time points. Baseline hypoxia as measured by both optical spectroscopy and EF5 immunofluorescence was variable, but on the basis of optical spectra, tumors appeared to become more hypoxic compared to normal skin after tourniquet application. The optical hypoxia signature was variable between patients while hemoglobin absorption increased. CONCLUSIONS: To our knowledge, this is the first use of real-time optical spectroscopy to evaluate oxygenation and perfusion within melanoma lesions during regional chemotherapy. We report our development of this new noninvasive means of assessing tumor vascular function, which has the potential to be a powerful tool for noninvasive examination of the melanoma tumor microenvironment.

Duke Scholars

Published In

Ann Surg Oncol

DOI

EISSN

1534-4681

Publication Date

May 2014

Volume

21

Issue

5

Start / End Page

1435 / 1440

Location

United States

Related Subject Headings

  • Prospective Studies
  • Prognosis
  • Pilot Projects
  • Oncology & Carcinogenesis
  • Neoplasm Staging
  • Middle Aged
  • Melphalan
  • Melanoma
  • Male
  • Indicators and Reagents
 

Citation

APA
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ICMJE
MLA
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Speicher, P. J., Beasley, G. M., Jiang, B., Lidsky, M. E., Palmer, G. M., Scarbrough, P. M., … Tyler, D. S. (2014). Hypoxia in melanoma: using optical spectroscopy and EF5 to assess tumor oxygenation before and during regional chemotherapy for melanoma. Ann Surg Oncol, 21(5), 1435–1440. https://doi.org/10.1245/s10434-013-3222-0
Speicher, Paul J., Georgia M. Beasley, Betty Jiang, Michael E. Lidsky, Gregory M. Palmer, Peter M. Scarbrough, Paul J. Mosca, Mark W. Dewhirst, and Douglas S. Tyler. “Hypoxia in melanoma: using optical spectroscopy and EF5 to assess tumor oxygenation before and during regional chemotherapy for melanoma.Ann Surg Oncol 21, no. 5 (May 2014): 1435–40. https://doi.org/10.1245/s10434-013-3222-0.
Speicher PJ, Beasley GM, Jiang B, Lidsky ME, Palmer GM, Scarbrough PM, et al. Hypoxia in melanoma: using optical spectroscopy and EF5 to assess tumor oxygenation before and during regional chemotherapy for melanoma. Ann Surg Oncol. 2014 May;21(5):1435–40.
Speicher, Paul J., et al. “Hypoxia in melanoma: using optical spectroscopy and EF5 to assess tumor oxygenation before and during regional chemotherapy for melanoma.Ann Surg Oncol, vol. 21, no. 5, May 2014, pp. 1435–40. Pubmed, doi:10.1245/s10434-013-3222-0.
Speicher PJ, Beasley GM, Jiang B, Lidsky ME, Palmer GM, Scarbrough PM, Mosca PJ, Dewhirst MW, Tyler DS. Hypoxia in melanoma: using optical spectroscopy and EF5 to assess tumor oxygenation before and during regional chemotherapy for melanoma. Ann Surg Oncol. 2014 May;21(5):1435–1440.
Journal cover image

Published In

Ann Surg Oncol

DOI

EISSN

1534-4681

Publication Date

May 2014

Volume

21

Issue

5

Start / End Page

1435 / 1440

Location

United States

Related Subject Headings

  • Prospective Studies
  • Prognosis
  • Pilot Projects
  • Oncology & Carcinogenesis
  • Neoplasm Staging
  • Middle Aged
  • Melphalan
  • Melanoma
  • Male
  • Indicators and Reagents