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Localization of Metal Electrodes in the Intact Rat Brain Using Registration of 3D Microcomputed Tomography Images to a Magnetic Resonance Histology Atlas.

Publication ,  Journal Article
Borg, JS; Vu, M-A; Badea, C; Badea, A; Johnson, GA; Dzirasa, K
Published in: eNeuro
2015

Simultaneous neural recordings taken from multiple areas of the rodent brain are garnering growing interest due to the insight they can provide about spatially distributed neural circuitry. The promise of such recordings has inspired great progress in methods for surgically implanting large numbers of metal electrodes into intact rodent brains. However, methods for localizing the precise location of these electrodes have remained severely lacking. Traditional histological techniques that require slicing and staining of physical brain tissue are cumbersome, and become increasingly impractical as the number of implanted electrodes increases. Here we solve these problems by describing a method that registers 3-D computerized tomography (CT) images of intact rat brains implanted with metal electrode bundles to a Magnetic Resonance Imaging Histology (MRH) Atlas. Our method allows accurate visualization of each electrode bundle's trajectory and location without removing the electrodes from the brain or surgically implanting external markers. In addition, unlike physical brain slices, once the 3D images of the electrode bundles and the MRH atlas are registered, it is possible to verify electrode placements from many angles by "re-slicing" the images along different planes of view. Further, our method can be fully automated and easily scaled to applications with large numbers of specimens. Our digital imaging approach to efficiently localizing metal electrodes offers a substantial addition to currently available methods, which, in turn, may help accelerate the rate at which insights are gleaned from rodent network neuroscience.

Duke Scholars

Published In

eNeuro

DOI

EISSN

2373-2822

Publication Date

2015

Volume

2

Issue

4

Location

United States

Related Subject Headings

  • 1109 Neurosciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Borg, J. S., Vu, M.-A., Badea, C., Badea, A., Johnson, G. A., & Dzirasa, K. (2015). Localization of Metal Electrodes in the Intact Rat Brain Using Registration of 3D Microcomputed Tomography Images to a Magnetic Resonance Histology Atlas. ENeuro, 2(4). https://doi.org/10.1523/ENEURO.0017-15.2015
Borg, Jana Schaich, Mai-Anh Vu, Cristian Badea, Alexandra Badea, G Allan Johnson, and Kafui Dzirasa. “Localization of Metal Electrodes in the Intact Rat Brain Using Registration of 3D Microcomputed Tomography Images to a Magnetic Resonance Histology Atlas.ENeuro 2, no. 4 (2015). https://doi.org/10.1523/ENEURO.0017-15.2015.
Borg, Jana Schaich, et al. “Localization of Metal Electrodes in the Intact Rat Brain Using Registration of 3D Microcomputed Tomography Images to a Magnetic Resonance Histology Atlas.ENeuro, vol. 2, no. 4, 2015. Pubmed, doi:10.1523/ENEURO.0017-15.2015.

Published In

eNeuro

DOI

EISSN

2373-2822

Publication Date

2015

Volume

2

Issue

4

Location

United States

Related Subject Headings

  • 1109 Neurosciences