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Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential.

Publication ,  Journal Article
Robles, FE; Deb, S; Wilson, JW; Gainey, CS; Selim, MA; Mosca, PJ; Tyler, DS; Fischer, MC; Warren, WS
Published in: Biomed Opt Express
September 1, 2015

Metastatic melanoma is associated with a poor prognosis, but no method reliably predicts which melanomas of a given stage will ultimately metastasize and which will not. While sentinel lymph node biopsy (SLNB) has emerged as the most powerful predictor of metastatic disease, the majority of people dying from metastatic melanoma still have a negative SLNB. Here we analyze pump-probe microscopy images of thin biopsy slides of primary melanomas to assess their metastatic potential. Pump-probe microscopy reveals detailed chemical information of melanin with subcellular spatial resolution. Quantification of the molecular signatures without reference standards is achieved using a geometrical representation of principal component analysis. Melanin structure is analyzed in unison with the chemical information by applying principles of mathematical morphology. Results show that melanin in metastatic primary lesions has lower chemical diversity than non-metastatic primary lesions, and contains two distinct phenotypes that are indicative of aggressive disease. Further, the mathematical morphology analysis reveals melanin in metastatic primary lesions has a distinct "dusty" quality. Finally, a statistical analysis shows that the combination of the chemical information with spatial structures predicts metastatic potential with much better sensitivity than SLNB and high specificity, suggesting pump-probe microscopy can be an important tool to help predict the metastatic potential of melanomas.

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Published In

Biomed Opt Express

DOI

ISSN

2156-7085

Publication Date

September 1, 2015

Volume

6

Issue

9

Start / End Page

3631 / 3645

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics
 

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Robles, F. E., Deb, S., Wilson, J. W., Gainey, C. S., Selim, M. A., Mosca, P. J., … Warren, W. S. (2015). Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential. Biomed Opt Express, 6(9), 3631–3645. https://doi.org/10.1364/BOE.6.003631
Robles, Francisco E., Sanghamitra Deb, Jesse W. Wilson, Christina S. Gainey, M Angelica Selim, Paul J. Mosca, Douglas S. Tyler, Martin C. Fischer, and Warren S. Warren. “Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential.Biomed Opt Express 6, no. 9 (September 1, 2015): 3631–45. https://doi.org/10.1364/BOE.6.003631.
Robles FE, Deb S, Wilson JW, Gainey CS, Selim MA, Mosca PJ, et al. Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential. Biomed Opt Express. 2015 Sep 1;6(9):3631–45.
Robles, Francisco E., et al. “Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential.Biomed Opt Express, vol. 6, no. 9, Sept. 2015, pp. 3631–45. Pubmed, doi:10.1364/BOE.6.003631.
Robles FE, Deb S, Wilson JW, Gainey CS, Selim MA, Mosca PJ, Tyler DS, Fischer MC, Warren WS. Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential. Biomed Opt Express. 2015 Sep 1;6(9):3631–3645.
Journal cover image

Published In

Biomed Opt Express

DOI

ISSN

2156-7085

Publication Date

September 1, 2015

Volume

6

Issue

9

Start / End Page

3631 / 3645

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics