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Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal 15N2-diazirine molecular tags

Publication ,  Journal Article
Theis, T; Ortiz, GX; Logan, AWJ; Claytor, KE; Feng, Y; Huhn, WP; Blum, V; Malcolmson, SJ; Chekmenev, EY; Wang, Q; Warren, WS
Published in: Science Advances
March 25, 2016

Conventional magnetic resonance (MR) faces serious sensitivity limitations which can be overcome by hyperpolarization methods, but the most common method (dynamic nuclear polarization) is complex and expensive, and applications are limited by short spin lifetimes (typically seconds) of biologically relevant molecules. We use a recently developed method, SABRE-SHEATH, to directly hyperpolarize 15N2 magnetization and long-lived 15N2 singlet spin order, with signal decay time constants of 5.8 and 23 minutes, respectively. We find >10,000-fold enhancements generating detectable nuclear MR signals that last for over an hour. 15N2-diazirines represent a class of particularly promising and versatile molecular tags, and can be incorporated into a wide range of biomolecules without significantly altering molecular function.

Duke Scholars

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Published In

Science Advances

DOI

Publication Date

March 25, 2016

Volume

2

Issue

3

Related Subject Headings

  • Nitrogen Isotopes
  • Models, Chemical
  • Magnetic Resonance Spectroscopy
  • Magnetic Fields
  • Electron Spin Resonance Spectroscopy
  • Diazomethane
 

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Theis, T., Ortiz, G. X., Logan, A. W. J., Claytor, K. E., Feng, Y., Huhn, W. P., … Warren, W. S. (2016). Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal 15N2-diazirine molecular tags. Science Advances, 2(3). https://doi.org/10.1126/sciadv.1501438
Theis, T., G. X. Ortiz, A. W. J. Logan, K. E. Claytor, Y. Feng, W. P. Huhn, V. Blum, et al. “Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal 15N2-diazirine molecular tags.” Science Advances 2, no. 3 (March 25, 2016). https://doi.org/10.1126/sciadv.1501438.
Theis T, Ortiz GX, Logan AWJ, Claytor KE, Feng Y, Huhn WP, et al. Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal 15N2-diazirine molecular tags. Science Advances. 2016 Mar 25;2(3).
Theis, T., et al. “Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal 15N2-diazirine molecular tags.” Science Advances, vol. 2, no. 3, Mar. 2016. Manual, doi:10.1126/sciadv.1501438.
Theis T, Ortiz GX, Logan AWJ, Claytor KE, Feng Y, Huhn WP, Blum V, Malcolmson SJ, Chekmenev EY, Wang Q, Warren WS. Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal 15N2-diazirine molecular tags. Science Advances. 2016 Mar 25;2(3).

Published In

Science Advances

DOI

Publication Date

March 25, 2016

Volume

2

Issue

3

Related Subject Headings

  • Nitrogen Isotopes
  • Models, Chemical
  • Magnetic Resonance Spectroscopy
  • Magnetic Fields
  • Electron Spin Resonance Spectroscopy
  • Diazomethane