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Early barriers to neonatal porcine islet engraftment in a dual transplant model.

Publication ,  Journal Article
Samy, KP; Davis, RP; Gao, Q; Martin, BM; Song, M; Cano, J; Farris, AB; McDonald, A; Gall, EK; Dove, CR; Leopardi, FV; How, T; Williams, KD ...
Published in: Am J Transplant
April 2018

Porcine islet xenografts have the potential to provide an inexhaustible source of islets for β cell replacement. Proof-of-concept has been established in nonhuman primates. However, significant barriers to xenoislet transplantation remain, including the poorly understood instant blood-mediated inflammatory reaction and a thorough understanding of early xeno-specific immune responses. A paucity of data exist comparing xeno-specific immune responses with alloislet (AI) responses in primates. We recently developed a dual islet transplant model, which enables direct histologic comparison of early engraftment immunobiology. In this study, we investigate early immune responses to neonatal porcine islet (NPI) xenografts compared with rhesus islet allografts at 1 hour, 24 hours, and 7 days. Within the first 24 hours after intraportal infusion, we identified greater apoptosis (caspase 3 activity and TUNEL [terminal deoxynucleotidyl transferase dUTP nick end labeling])-positive cells) of NPIs compared with AIs. Macrophage infiltration was significantly greater at 24 hours compared with 1 hour in both NPI (wild-type) and AIs. At 7 days, IgM and macrophages were highly specific for NPIs (α1,3-galactosyltransferase knockout) compared with AIs. These findings demonstrate an augmented macrophage and antibody response toward xenografts compared with allografts. These data may inform future immune or genetic manipulations required to improve xenoislet engraftment.

Duke Scholars

Published In

Am J Transplant

DOI

EISSN

1600-6143

Publication Date

April 2018

Volume

18

Issue

4

Start / End Page

998 / 1006

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Swine
  • Surgery
  • Macrophages
  • Macaca mulatta
  • Islets of Langerhans Transplantation
  • Islets of Langerhans
  • Inflammation
  • Graft Survival
  • Graft Rejection
 

Citation

APA
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MLA
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Samy, K. P., Davis, R. P., Gao, Q., Martin, B. M., Song, M., Cano, J., … Kirk, A. D. (2018). Early barriers to neonatal porcine islet engraftment in a dual transplant model. Am J Transplant, 18(4), 998–1006. https://doi.org/10.1111/ajt.14601
Samy, K. P., R. P. Davis, Q. Gao, B. M. Martin, M. Song, J. Cano, A. B. Farris, et al. “Early barriers to neonatal porcine islet engraftment in a dual transplant model.Am J Transplant 18, no. 4 (April 2018): 998–1006. https://doi.org/10.1111/ajt.14601.
Samy KP, Davis RP, Gao Q, Martin BM, Song M, Cano J, et al. Early barriers to neonatal porcine islet engraftment in a dual transplant model. Am J Transplant. 2018 Apr;18(4):998–1006.
Samy, K. P., et al. “Early barriers to neonatal porcine islet engraftment in a dual transplant model.Am J Transplant, vol. 18, no. 4, Apr. 2018, pp. 998–1006. Pubmed, doi:10.1111/ajt.14601.
Samy KP, Davis RP, Gao Q, Martin BM, Song M, Cano J, Farris AB, McDonald A, Gall EK, Dove CR, Leopardi FV, How T, Williams KD, Devi GR, Collins BH, Kirk AD. Early barriers to neonatal porcine islet engraftment in a dual transplant model. Am J Transplant. 2018 Apr;18(4):998–1006.
Journal cover image

Published In

Am J Transplant

DOI

EISSN

1600-6143

Publication Date

April 2018

Volume

18

Issue

4

Start / End Page

998 / 1006

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Swine
  • Surgery
  • Macrophages
  • Macaca mulatta
  • Islets of Langerhans Transplantation
  • Islets of Langerhans
  • Inflammation
  • Graft Survival
  • Graft Rejection