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Intravital imaging of mouse embryos.

Publication ,  Journal Article
Huang, Q; Cohen, MA; Alsina, FC; Devlin, G; Garrett, A; McKey, J; Havlik, P; Rakhilin, N; Wang, E; Xiang, K; Mathews, P; Wang, L; Bock, C ...
Published in: Science
April 10, 2020

Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.

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Published In

Science

DOI

EISSN

1095-9203

Publication Date

April 10, 2020

Volume

368

Issue

6487

Start / End Page

181 / 186

Location

United States

Related Subject Headings

  • Uterus
  • Synaptic Transmission
  • Retina
  • Pregnancy
  • Placenta
  • Neural Crest
  • Neovascularization, Physiologic
  • Mice, Transgenic
  • Mice
  • Intravital Microscopy
 

Citation

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Huang, Q., Cohen, M. A., Alsina, F. C., Devlin, G., Garrett, A., McKey, J., … Shen, X. (2020). Intravital imaging of mouse embryos. Science, 368(6487), 181–186. https://doi.org/10.1126/science.aba0210
Huang, Qiang, Malkiel A. Cohen, Fernando C. Alsina, Garth Devlin, Aliesha Garrett, Jennifer McKey, Patrick Havlik, et al. “Intravital imaging of mouse embryos.Science 368, no. 6487 (April 10, 2020): 181–86. https://doi.org/10.1126/science.aba0210.
Huang Q, Cohen MA, Alsina FC, Devlin G, Garrett A, McKey J, et al. Intravital imaging of mouse embryos. Science. 2020 Apr 10;368(6487):181–6.
Huang, Qiang, et al. “Intravital imaging of mouse embryos.Science, vol. 368, no. 6487, Apr. 2020, pp. 181–86. Pubmed, doi:10.1126/science.aba0210.
Huang Q, Cohen MA, Alsina FC, Devlin G, Garrett A, McKey J, Havlik P, Rakhilin N, Wang E, Xiang K, Mathews P, Wang L, Bock C, Ruthig V, Wang Y, Negrete M, Wong CW, Murthy PKL, Zhang S, Daniel AR, Kirsch DG, Kang Y, Capel B, Asokan A, Silver DL, Jaenisch R, Shen X. Intravital imaging of mouse embryos. Science. 2020 Apr 10;368(6487):181–186.
Journal cover image

Published In

Science

DOI

EISSN

1095-9203

Publication Date

April 10, 2020

Volume

368

Issue

6487

Start / End Page

181 / 186

Location

United States

Related Subject Headings

  • Uterus
  • Synaptic Transmission
  • Retina
  • Pregnancy
  • Placenta
  • Neural Crest
  • Neovascularization, Physiologic
  • Mice, Transgenic
  • Mice
  • Intravital Microscopy