Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.
Publication
, Journal Article
Forrester, MT; Thompson, JW; Foster, MW; Nogueira, L; Moseley, MA; Stamler, JS
Published in: Nat Biotechnol
June 2009
We have modified the biotin switch assay for protein S-nitrosothiols (SNOs), using resin-assisted capture (SNO-RAC). Compared with existing methodologies, SNO-RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates identification and quantification of S-nitrosylated sites by mass spectrometry. When combined with iTRAQ labeling, SNO-RAC revealed that intracellular proteins may undergo rapid denitrosylation on a global scale. This methodology is readily adapted to analyzing diverse cysteine-based protein modifications, including S-acylation.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Nat Biotechnol
DOI
EISSN
1546-1696
Publication Date
June 2009
Volume
27
Issue
6
Start / End Page
557 / 559
Location
United States
Related Subject Headings
- Sulfhydryl Compounds
- Sensitivity and Specificity
- S-Nitrosothiols
- Proteomics
- Proteome
- Mass Spectrometry
- Humans
- Glyceraldehyde-3-Phosphate Dehydrogenases
- Cysteine
- Cell Line
Citation
APA
Chicago
ICMJE
MLA
NLM
Forrester, M. T., Thompson, J. W., Foster, M. W., Nogueira, L., Moseley, M. A., & Stamler, J. S. (2009). Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture. Nat Biotechnol, 27(6), 557–559. https://doi.org/10.1038/nbt.1545
Forrester, Michael T., J Will Thompson, Matthew W. Foster, Leonardo Nogueira, M Arthur Moseley, and Jonathan S. Stamler. “Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.” Nat Biotechnol 27, no. 6 (June 2009): 557–59. https://doi.org/10.1038/nbt.1545.
Forrester MT, Thompson JW, Foster MW, Nogueira L, Moseley MA, Stamler JS. Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture. Nat Biotechnol. 2009 Jun;27(6):557–9.
Forrester, Michael T., et al. “Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.” Nat Biotechnol, vol. 27, no. 6, June 2009, pp. 557–59. Pubmed, doi:10.1038/nbt.1545.
Forrester MT, Thompson JW, Foster MW, Nogueira L, Moseley MA, Stamler JS. Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture. Nat Biotechnol. 2009 Jun;27(6):557–559.
Published In
Nat Biotechnol
DOI
EISSN
1546-1696
Publication Date
June 2009
Volume
27
Issue
6
Start / End Page
557 / 559
Location
United States
Related Subject Headings
- Sulfhydryl Compounds
- Sensitivity and Specificity
- S-Nitrosothiols
- Proteomics
- Proteome
- Mass Spectrometry
- Humans
- Glyceraldehyde-3-Phosphate Dehydrogenases
- Cysteine
- Cell Line