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High-resolution in vivo imaging of blood vessels without labeling.

Publication ,  Journal Article
Fu, D; Ye, T; Matthews, TE; Chen, BJ; Yurtserver, G; Warren, WS
Published in: Opt Lett
September 15, 2007

We demonstrate that both oxyhemoglobin and deoxyhemoglobin have sequential two-color, two-photon absorption properties that can serve as endogenous contrasts in microvasculature imaging. Using a sensitive modulation transfer technique, we are able to image hemoglobin in red blood cells with micrometer resolution, both in vitro and in vivo. We show that excellent contrast from hemoglobin without any labeling can be obtained in tissue.

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Published In

Opt Lett

DOI

ISSN

0146-9592

Publication Date

September 15, 2007

Volume

32

Issue

18

Start / End Page

2641 / 2643

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Optics
  • Microscopy, Fluorescence, Multiphoton
  • Mice
  • Hemoglobins
  • Erythrocytes
  • Cells, Cultured
  • Blood Vessels
  • Animals
  • 5102 Atomic, molecular and optical physics
 

Citation

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Fu, D., Ye, T., Matthews, T. E., Chen, B. J., Yurtserver, G., & Warren, W. S. (2007). High-resolution in vivo imaging of blood vessels without labeling. Opt Lett, 32(18), 2641–2643. https://doi.org/10.1364/ol.32.002641
Fu, Dan, Tong Ye, Thomas E. Matthews, Benny J. Chen, Gunay Yurtserver, and Warren S. Warren. “High-resolution in vivo imaging of blood vessels without labeling.Opt Lett 32, no. 18 (September 15, 2007): 2641–43. https://doi.org/10.1364/ol.32.002641.
Fu D, Ye T, Matthews TE, Chen BJ, Yurtserver G, Warren WS. High-resolution in vivo imaging of blood vessels without labeling. Opt Lett. 2007 Sep 15;32(18):2641–3.
Fu, Dan, et al. “High-resolution in vivo imaging of blood vessels without labeling.Opt Lett, vol. 32, no. 18, Sept. 2007, pp. 2641–43. Pubmed, doi:10.1364/ol.32.002641.
Fu D, Ye T, Matthews TE, Chen BJ, Yurtserver G, Warren WS. High-resolution in vivo imaging of blood vessels without labeling. Opt Lett. 2007 Sep 15;32(18):2641–2643.
Journal cover image

Published In

Opt Lett

DOI

ISSN

0146-9592

Publication Date

September 15, 2007

Volume

32

Issue

18

Start / End Page

2641 / 2643

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Optics
  • Microscopy, Fluorescence, Multiphoton
  • Mice
  • Hemoglobins
  • Erythrocytes
  • Cells, Cultured
  • Blood Vessels
  • Animals
  • 5102 Atomic, molecular and optical physics