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RNA-guided gene activation by CRISPR-Cas9-based transcription factors.

Publication ,  Journal Article
Perez-Pinera, P; Kocak, DD; Vockley, CM; Adler, AF; Kabadi, AM; Polstein, LR; Thakore, PI; Glass, KA; Ousterout, DG; Leong, KW; Guilak, F ...
Published in: Nat Methods
October 2013

Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.

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Published In

Nat Methods

DOI

EISSN

1548-7105

Publication Date

October 2013

Volume

10

Issue

10

Start / End Page

973 / 976

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Transcription Factors
  • Ribonucleases
  • RNA, Small Untranslated
  • RNA Editing
  • Protein Engineering
  • Interleukin 1 Receptor Antagonist Protein
  • Humans
  • High-Throughput Nucleotide Sequencing
  • HEK293 Cells
 

Citation

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Perez-Pinera, P., Kocak, D. D., Vockley, C. M., Adler, A. F., Kabadi, A. M., Polstein, L. R., … Gersbach, C. A. (2013). RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods, 10(10), 973–976. https://doi.org/10.1038/nmeth.2600
Perez-Pinera, Pablo, D Dewran Kocak, Christopher M. Vockley, Andrew F. Adler, Ami M. Kabadi, Lauren R. Polstein, Pratiksha I. Thakore, et al. “RNA-guided gene activation by CRISPR-Cas9-based transcription factors.Nat Methods 10, no. 10 (October 2013): 973–76. https://doi.org/10.1038/nmeth.2600.
Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, et al. RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Oct;10(10):973–6.
Perez-Pinera, Pablo, et al. “RNA-guided gene activation by CRISPR-Cas9-based transcription factors.Nat Methods, vol. 10, no. 10, Oct. 2013, pp. 973–76. Pubmed, doi:10.1038/nmeth.2600.
Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Oct;10(10):973–976.

Published In

Nat Methods

DOI

EISSN

1548-7105

Publication Date

October 2013

Volume

10

Issue

10

Start / End Page

973 / 976

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Transcription Factors
  • Ribonucleases
  • RNA, Small Untranslated
  • RNA Editing
  • Protein Engineering
  • Interleukin 1 Receptor Antagonist Protein
  • Humans
  • High-Throughput Nucleotide Sequencing
  • HEK293 Cells