RNA-guided gene activation by CRISPR-Cas9-based transcription factors.
Publication
, Journal Article
Perez-Pinera, P; Kocak, DD; Vockley, CM; Adler, AF; Kabadi, AM; Polstein, LR; Thakore, PI; Glass, KA; Ousterout, DG; Leong, KW; Guilak, F ...
Published in: Nat Methods
October 2013
Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.
Duke Scholars
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Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
October 2013
Volume
10
Issue
10
Start / End Page
973 / 976
Location
United States
Related Subject Headings
- Transcriptional Activation
- Transcription Factors
- Ribonucleases
- RNA, Small Untranslated
- RNA Editing
- Protein Engineering
- Interleukin 1 Receptor Antagonist Protein
- Humans
- High-Throughput Nucleotide Sequencing
- HEK293 Cells
Citation
APA
Chicago
ICMJE
MLA
NLM
Perez-Pinera, P., Kocak, D. D., Vockley, C. M., Adler, A. F., Kabadi, A. M., Polstein, L. R., … Gersbach, C. A. (2013). RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods, 10(10), 973–976. https://doi.org/10.1038/nmeth.2600
Perez-Pinera, Pablo, D Dewran Kocak, Christopher M. Vockley, Andrew F. Adler, Ami M. Kabadi, Lauren R. Polstein, Pratiksha I. Thakore, et al. “RNA-guided gene activation by CRISPR-Cas9-based transcription factors.” Nat Methods 10, no. 10 (October 2013): 973–76. https://doi.org/10.1038/nmeth.2600.
Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, et al. RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Oct;10(10):973–6.
Perez-Pinera, Pablo, et al. “RNA-guided gene activation by CRISPR-Cas9-based transcription factors.” Nat Methods, vol. 10, no. 10, Oct. 2013, pp. 973–76. Pubmed, doi:10.1038/nmeth.2600.
Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Oct;10(10):973–976.
Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
October 2013
Volume
10
Issue
10
Start / End Page
973 / 976
Location
United States
Related Subject Headings
- Transcriptional Activation
- Transcription Factors
- Ribonucleases
- RNA, Small Untranslated
- RNA Editing
- Protein Engineering
- Interleukin 1 Receptor Antagonist Protein
- Humans
- High-Throughput Nucleotide Sequencing
- HEK293 Cells