Tatiana Segura
Professor of Biomedical Engineering
Professor Tatiana Segura received her BS degree in Bioengineering from the University of California Berkeley and her doctorate in Chemical Engineering from Northwestern University. Her graduate work in designing and understanding non-viral gene delivery from hydrogel scaffolds was supervised by Prof. Lonnie Shea. She pursued post-doctoral training at the Swiss Federal Institute of Technology, Lausanne under the guidance of Prof. Jeffrey Hubbell, where her focus was self-assembled polymer systems for gene and drug delivery. Professor Segura's Laboratory studies the use of materials for minimally invasive in situ tissue repair. On this topic, she has published 113 peered reviewed publications to date. She has been recognized with the Outstanding Young Investigator Award from the American Society of Gene and Cell Therapy, the American Heart Association National Scientist Development Grant, and the CAREER award from National Science Foundation. She was Elected to the College of Fellows at the American Institute for Medical and Biological Engineers (AIMBE) in 2017. She spent the first 11 years of her career at UCLA department of Chemical and Biomolecular Engineering and has recently relocated to Duke University, where she holds appointments in Biomedical Engineering, Neurology and Dermatology.
Current Research Interests
The design of biomaterials to promote endogenous repair and reducing inflammation through the design of the geometry of the material, and delivering genes, proteins and drugs.
Current Appointments & Affiliations
- Professor of Biomedical Engineering, Biomedical Engineering, Pratt School of Engineering 2018
- Professor in Neurology, Neurology, Clinical Science Departments 2019
- Professor in Dermatology, Dermatology, Clinical Science Departments 2021
- Affiliate of the Duke Regeneration Center, Regeneration Next Initiative, Basic Science Departments 2021
Contact Information
- 534 Research Drive, Durham, NC 27708
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tatiana.segura@duke.edu
(919) 660-2901
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@seguralab Twitter feed
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Segura Lab website
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Tatiana Segura on LinkedIn
- Background
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Education, Training, & Certifications
- Ph.D., Northwestern University 2004
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Previous Appointments & Affiliations
- Visiting Professor in the Department of Biomedical Engineering, Biomedical Engineering, Pratt School of Engineering 2017 - 2018
- Recognition
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In the News
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MAR 2, 2022 -
SEP 27, 2021 Pratt School of Engineering -
FEB 10, 2021 -
NOV 11, 2020 Pratt School of Engineering -
NOV 5, 2019 Pratt School of Engineering -
MAY 21, 2018 Pratt School of Engineering -
AUG 22, 2017 Pratt School of Engineering
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Awards & Honors
- Research
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Selected Grants
- University Training Program in Biomolecular and Tissue Engineering awarded by National Institutes of Health 1994 - 2027
- Development and Application of New Ionization Methods for Biological Mass Spectrometry awarded by North Carolina State University 2022 - 2026
- Duke Center for Advancement of Child Health (CAtCH). awarded by National Institutes of Health 2021 - 2026
- Pharmacological Sciences Training Grant awarded by National Institutes of Health 2020 - 2025
- Engineering Adaptive Immune Responses from Hydrogel Scaffolds to Promote Tissue Regeneration awarded by National Institutes of Health 2020 - 2025
- Biomaterials to promote synapse formation after stroke awarded by National Institutes of Health 2020 - 2024
- VEGF ligand presentation and therapeutic angiogenesis awarded by National Institutes of Health 2020 - 2024
- Brain repair after stroke awarded by Centre for Effective Altruism 2022 - 2023
- Non-Canonical Pathways for Electrogene Transfer awarded by National Institutes of Health 2018 - 2022
- Cell and Gene Replacement Strategies for Arginase Deficiency awarded by University of California - Los Angeles 2018 - 2022
- Pharmacological Sciences Training Program awarded by National Institutes of Health 1975 - 2020
- Engineering Injectable Microporous Hydrogels for Brain Repair awarded by National Institutes of Health 2018 - 2020
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External Relationships
- Tempo therapeutics
- Publications & Artistic Works
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Selected Publications
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Academic Articles
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Anderson, Alexa R., and Tatiana Segura. “Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture.” Journal of Visualized Experiments : Jove, no. 188 (October 2022). https://doi.org/10.3791/64554.Full Text
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Anderson, Alexa R., Ethan Nicklow, and Tatiana Segura. “Particle fraction is a bioactive cue in granular scaffolds.” Acta Biomaterialia 150 (September 2022): 111–27. https://doi.org/10.1016/j.actbio.2022.07.051.Full Text
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Sideris, Elias, Sophia Kioulaphides, Katrina Wilson, Aaron Yu, Jun Chen, S Thomas Carmichael, and Tatiana Segura. “Particle hydrogels decrease cerebral atrophy and attenuate astrocyte and microglia/macrophage reactivity after stroke.” Advanced Therapeutics 5, no. 8 (August 2022): 2200048. https://doi.org/10.1002/adtp.202200048.Full Text
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Wilson, Katrina L., Sasha Cai Lesher Pérez, Moawiah M. Naffaa, Sean H. Kelly, and Tatiana Segura. “Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds.” Advanced Materials (Deerfield Beach, Fla.) 34, no. 33 (August 2022): e2201921. https://doi.org/10.1002/adma.202201921.Full Text
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Kurt, Evan, and Tatiana Segura. “Nucleic Acid Delivery from Granular Hydrogels.” Advanced Healthcare Materials 11, no. 3 (February 2022): e2101867. https://doi.org/10.1002/adhm.202101867.Full Text
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Samal, Juhi, and Tatiana Segura. “Injectable biomaterial shuttles for cell therapy in stroke.” Brain Research Bulletin 176 (November 2021): 25–42. https://doi.org/10.1016/j.brainresbull.2021.08.002.Full Text
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Xi, Weixian, Vishal Hegde, Stephen D. Zoller, Howard Y. Park, Christopher M. Hart, Takeru Kondo, Christopher D. Hamad, et al. “Point-of-care antimicrobial coating protects orthopaedic implants from bacterial challenge.” Nature Communications 12, no. 1 (September 2021): 5473. https://doi.org/10.1038/s41467-021-25383-z.Full Text
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Griffin, Donald R., Maani M. Archang, Chen-Hsiang Kuan, Westbrook M. Weaver, Jason S. Weinstein, An Chieh Feng, Amber Ruccia, et al. “Activating an adaptive immune response from a hydrogel scaffold imparts regenerative wound healing.” Nature Materials 20, no. 4 (April 2021): 560–69. https://doi.org/10.1038/s41563-020-00844-w.Full Text Open Access Copy
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Escuin-Ordinas, Helena, Yining Liu, Lu Sun, Willy Hugo, Robert Dimatteo, Rong Rong Huang, Paige Krystofinski, et al. “Wound healing with topical BRAF inhibitor therapy in a diabetic model suggests tissue regenerative effects.” Plos One 16, no. 6 (January 2021): e0252597. https://doi.org/10.1371/journal.pone.0252597.Full Text
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Anderson, Alexa R., and Tatiana Segura. “Injectable biomaterials for treatment of glioblastoma.” Advanced Materials Interfaces 7, no. 20 (October 2020): 2001055. https://doi.org/10.1002/admi.202001055.Full Text
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Wilson, Katrina L., S Thomas Carmichael, and Tatiana Segura. “Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke.” Journal of Visualized Experiments : Jove, no. 164 (October 2020). https://doi.org/10.3791/61450.Full Text
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McMillan, Alexander H., Emma K. Thomée, Alessandra Dellaquila, Hussam Nassman, Tatiana Segura, and Sasha Cai Lesher-Pérez. “Rapid Fabrication of Membrane-Integrated Thermoplastic Elastomer Microfluidic Devices.” Micromachines 11, no. 8 (July 2020): E731. https://doi.org/10.3390/mi11080731.Full Text
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Erning, Kevin, and Tatiana Segura. “Materials to Promote Recovery After Stroke.” Current Opinion in Biomedical Engineering 14 (June 2020): 9–17. https://doi.org/10.1016/j.cobme.2020.04.002.Full Text Open Access Copy
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Darling, Nicole J., Weixian Xi, Elias Sideris, Alexa R. Anderson, Cassie Pong, S Thomas Carmichael, and Tatiana Segura. “Click by Click Microporous Annealed Particle (MAP) Scaffolds.” Advanced Healthcare Materials 9, no. 10 (May 2020): e1901391. https://doi.org/10.1002/adhm.201901391.Full Text
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Hegde, Vishal, Howard Y. Park, Erik Dworsky, Stephen D. Zoller, Weixian Xi, Daniel O. Johansen, Amanda H. Loftin, Christopher D. Hamad, Tatiana Segura, and Nicholas M. Bernthal. “The Use of a Novel Antimicrobial Implant Coating In Vivo to Prevent Spinal Implant Infection.” Spine 45, no. 6 (March 2020): E305–11. https://doi.org/10.1097/brs.0000000000003279.Full Text
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Daly, Andrew C., Lindsay Riley, Tatiana Segura, and Jason A. Burdick. “Hydrogel microparticles for biomedical applications.” Nature Reviews. Materials 5, no. 1 (January 2020): 20–43. https://doi.org/10.1038/s41578-019-0148-6.Full Text
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Liu, Yining, and Tatiana Segura. “Biomaterials-Mediated Regulation of Macrophage Cell Fate.” Frontiers in Bioengineering and Biotechnology 8 (January 2020): 609297. https://doi.org/10.3389/fbioe.2020.609297.Full Text Open Access Copy
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Riley, Lindsay, Lucas Schirmer, and Tatiana Segura. “Granular hydrogels: emergent properties of jammed hydrogel microparticles and their applications in tissue repair and regeneration.” Current Opinion in Biotechnology 60 (December 2019): 1–8. https://doi.org/10.1016/j.copbio.2018.11.001.Full Text
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Koh, Jaekyung, Donald R. Griffin, Maani M. Archang, An-Chieh Feng, Thomas Horn, Michael Margolis, David Zalazar, Tatiana Segura, Philip O. Scumpia, and Dino Di Carlo. “Enhanced In Vivo Delivery of Stem Cells using Microporous Annealed Particle Scaffolds.” Small (Weinheim an Der Bergstrasse, Germany) 15, no. 39 (September 2019): e1903147. https://doi.org/10.1002/smll.201903147.Full Text Open Access Copy
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Stefanelli, Victoria L., Shilpa Choudhury, Ping Hu, Yining Liu, Anja Schwenzer, Chiuan-Ren Yeh, Dwight M. Chambers, et al. “Citrullination of fibronectin alters integrin clustering and focal adhesion stability promoting stromal cell invasion.” Matrix Biology : Journal of the International Society for Matrix Biology 82 (September 2019): 86–104. https://doi.org/10.1016/j.matbio.2019.04.002.Full Text
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Truong, Norman F., Evan Kurt, Nairi Tahmizyan, Sasha Cai Lesher-Pérez, Mabel Chen, Nicole J. Darling, Weixian Xi, and Tatiana Segura. “Microporous annealed particle hydrogel stiffness, void space size, and adhesion properties impact cell proliferation, cell spreading, and gene transfer.” Acta Biomaterialia 94 (August 2019): 160–72. https://doi.org/10.1016/j.actbio.2019.02.054.Full Text
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Truong, Norman F., Sasha Cai Lesher-Pérez, Evan Kurt, and Tatiana Segura. “Pathways Governing Polyethylenimine Polyplex Transfection in Microporous Annealed Particle Scaffolds.” Bioconjugate Chemistry 30, no. 2 (February 2019): 476–86. https://doi.org/10.1021/acs.bioconjchem.8b00696.Full Text
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Pannier, Angela K., Tyler Kozisek, and Tatiana Segura. “Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles.” Methods in Molecular Biology (Clifton, N.J.) 1943 (January 2019): 177–97. https://doi.org/10.1007/978-1-4939-9092-4_12.Full Text
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Stavrakis, A. I., S. Zhu, A. H. Loftin, X. Weixian, J. Niska, V. Hegde, T. Segura, and N. M. Bernthal. “Controlled Release of Vancomycin and Tigecycline from an Orthopaedic Implant Coating Prevents Staphylococcus aureus Infection in an Open Fracture Animal Model.” Biomed Research International 2019 (January 2019): 1638508. https://doi.org/10.1155/2019/1638508.Full Text Open Access Copy
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Darling, Nicole, Elias Sideris, Naomi Hamada, and Tatiana Segura. “Injectable and spatially patterned microporous annealed particle (MAP) hydrogels for tissue repair applications.” Advanced Science, October 11, 2018, 1–8. https://doi.org/10.1002/advs.201801046.Full Text Link to Item
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Villate-Beitia, Ilia, Norman F. Truong, Idoia Gallego, Jon Zárate, Gustavo Puras, José Luis Pedraz, and Tatiana Segura. “Hyaluronic acid hydrogel scaffolds loaded with cationic niosomes for efficient non-viral gene delivery.” Rsc Advances 8, no. 56 (September 2018): 31934–42. https://doi.org/10.1039/c8ra05125a.Full Text
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Youngblood, Richard L., Norman F. Truong, Tatiana Segura, and Lonnie D. Shea. “It's All in the Delivery: Designing Hydrogels for Cell and Non-viral Gene Therapies.” Molecular Therapy : The Journal of the American Society of Gene Therapy 26, no. 9 (September 2018): 2087–2106. https://doi.org/10.1016/j.ymthe.2018.07.022.Full Text
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Nih, Lina R., Shiva Gojgini, S Thomas Carmichael, and Tatiana Segura. “Dual-function injectable angiogenic biomaterial for the repair of brain tissue following stroke.” Nature Materials 17, no. 7 (July 2018): 642–51. https://doi.org/10.1038/s41563-018-0083-8.Full Text
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Zhu, Suwei, Shuoran Li, Helena Escuin-Ordinas, Robert Dimatteo, Weixian Xi, Antoni Ribas, and Tatiana Segura. “Accelerated wound healing by injectable star poly(ethylene glycol)-b-poly(propylene sulfide) scaffolds loaded with poorly water-soluble drugs.” Journal of Controlled Release : Official Journal of the Controlled Release Society 282 (July 2018): 156–65. https://doi.org/10.1016/j.jconrel.2018.05.006.Full Text
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Dimatteo, Robert, Nicole J. Darling, and Tatiana Segura. “In situ forming injectable hydrogels for drug delivery and wound repair.” Advanced Drug Delivery Reviews 127 (March 2018): 167–84. https://doi.org/10.1016/j.addr.2018.03.007.Full Text
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Truong, Norman F., and Tatiana Segura. “Sustained Transgene Expression via Hydrogel-Mediated Gene Transfer Results from Multiple Transfection Events.” Acs Biomaterials Science & Engineering 4, no. 3 (March 2018): 981–87. https://doi.org/10.1021/acsbiomaterials.7b00957.Full Text
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Li, S., L. R. Nih, H. Bachman, P. Fei, Y. Li, E. Nam, R. Dimatteo, S. T. Carmichael, T. H. Barker, and T. Segura. “Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability.” Nature Materials 16, no. 9 (September 1, 2017): 953–61. https://doi.org/10.1038/nmat4954.Full Text
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Ding, Yichen, Arash Abiri, Parinaz Abiri, Shuoran Li, Chih-Chiang Chang, Kyung In Baek, Jeffrey J. Hsu, et al. “Integrating light-sheet imaging with virtual reality to recapitulate developmental cardiac mechanics.” Jci Insight 2 (2017).
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Nih, Lina R., Elias Sideris, S Thomas Carmichael, and Tatiana Segura. “Injection of Microporous Annealing Particle (MAP) Hydrogels in the Stroke Cavity Reduces Gliosis and Inflammation and Promotes NPC Migration to the Lesion.” Advanced Materials 29 (2017).
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Nih, Lina R., Pouria Moshayedi, Irene L. Llorente, Andrew R. Berg, Jessica Cinkornpumin, William E. Lowry, Tatiana Segura, and S Thomas Carmichael. “Engineered HA hydrogel for stem cell transplantation in the brain: Biocompatibility data using a design of experiment approach.” Data in Brief 10 (2017): 202–9.
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Ray, Aniruddha, Shuoran Li, Tatiana Segura, and Aydogan Ozcan. “High-throughput quantification of nanoparticle degradation using computational microscopy and its application to drug delivery nanocapsules.” Acs Photonics 4 (2017): 1216–24.
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Zhu, Xiaolu, Shiva Gojgini, Ting-Hsuan Chen, Peng Fei, Siyan Dong, Chih-Ming Ho, and Tatiana Segura. “Directing three-dimensional multicellular morphogenesis by self-organization of vascular mesenchymal cells in hyaluronic acid hydrogels.” Journal of Biological Engineering 11 (2017): 12–12.
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Darling, Nicole J., Yiu-Sun Hung, Shruti Sharma, and Tatiana Segura. “Controlling the kinetics of thiol-maleimide Michael-type addition gelation kinetics for the generation of homogenous poly (ethylene glycol) hydrogels.” Biomaterials 101 (2016): 199–206.
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Escuin-Ordinas, Helena, Shuoran Li, Michael W. Xie, Lu Sun, Willy Hugo, Rong Rong Huang, Jing Jiao, et al. “Cutaneous wound healing through paradoxical MAPK activation by BRAF inhibitors.” Nature Communications 7 (2016).
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Lesher-Perez, Sasha Cai, Tatiana Segura, and Christopher Moraes. “Getting there is half the battle: recent advances in delivering therapeutics.” Integrative Biology 8 (2016): 8–11.
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Li, Jie, Linlin Zhang, Yang Liu, Jing Wen, Di Wu, Duo Xu, Tatiana Segura, Jing Jin, Yunfeng Lu, and Hui Wang. “An intracellular protein delivery platform based on glutathione-responsive protein nanocapsules.” Chemical Communications 52 (2016): 13608–11.
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Moshayedi, Pouria, Lina R. Nih, Irene L. Llorente, Andrew R. Berg, Jessica Cinkornpumin, William E. Lowry, Tatiana Segura, and S Thomas Carmichael. “Systematic optimization of an engineered hydrogel allows for selective control of human neural stem cell survival and differentiation after transplantation in the stroke brain.” Biomaterials 105 (2016): 145–55.
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Nih, Lina Ratiba, Stanley Thomas Carmichael, and Tatiana Segura. “Hydrogels for brain repair after stroke: an emerging treatment option.” Current Opinion in Biotechnology 40 (2016): 155–63.
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Sideris, Elias, Donald R. Griffin, Yichen Ding, Shuoran Li, Westbrook M. Weaver, Dino Di Carlo, Tzung Hsiai, and Tatiana Segura. “Particle hydrogels based on hyaluronic acid building blocks.” Acs Biomaterials Science & Engineering 2 (2016): 2034–41.
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Stavrakis, Alexandra I., Suwei Zhu, Vishal Hegde, Amanda H. Loftin, Alyssa G. Ashbaugh, Jared A. Niska, Lloyd S. Miller, Tatiana Segura, and Nicholas M. Bernthal. “In Vivo Efficacy of a ´93Smart´94 Antimicrobial Implant Coating.” Jbjs 98 (2016): 1183–89.
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Zhu, Suwei, and Tatiana Segura. “Cell-Demanded VEGF Release via Nanocapsules Elicits Different Receptor Activation Dynamics and Enhanced Angiogenesis.” Annals of Biomedical Engineering 44 (2016): 1983–92.
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Zhu, Xiaolu, Shiva Gojgini, Ting-Hsuan Chen, Fang Teng, Peng Fei, Siyan Dong, Tatiana Segura, and Chih-Ming Ho. “Three dimensional tubular structure self-assembled by vascular mesenchymal cells at stiffness interfaces of hydrogels.” Biomedicine & Pharmacotherapy 83 (2016): 1203–11.
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Du, J., J. Jin, Y. Liu, J. Li, T. Tokatlian, Z. Lu, T. Segura, X. B. Yuan, X. Yang, and Y. Lu. “Erratum: Gold-nanocrystal-enhanced bioluminescent nanocapsules (ACS Nano (2014) 8 (9964-9969) DOI: 10.1021/nn504371h).” Acs Nano 9, no. 2 (February 24, 2015): 2213. https://doi.org/10.1021/acsnano.5b00374.Full Text
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Boehnke, Natalie, Cynthia Cam, Erhan Bat, Tatiana Segura, and Heather D. Maynard. “Imine hydrogels with tunable degradability for tissue engineering.” Biomacromolecules 16 (2015): 2101–8.
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Cam, C., S. Zhu, N. F. Truong, P. O. Scumpia, and T. Segura. “Systematic evaluation of natural scaffolds in cutaneous wound healing.” Journal of Materials Chemistry B 3, no. 40 (January 1, 2015): 7986–92. https://doi.org/10.1039/c5tb00807g.Full Text
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Du, Juanjuan, Jing Jin, Yang Liu, Jie Li, Talar Tokatlian, Zuhong Lu, Tatiana Segura, Xu-bo Yuan, Xianjin Yang, and Yunfeng Lu. “Correction to Gold-Nanocrystal-Enhanced Bioluminescent Nanocapsules.” Acs Nano 9 (2015): 2213–2213.
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Griffin, Donald R., Westbrook M. Weaver, Philip O. Scumpia, Dino Di Carlo, and Tatiana Segura. “Accelerated wound healing by injectable microporous gel scaffolds assembled from annealed building blocks.” Nature Materials 14 (2015): 737–44.
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Lam, Jonathan, S Thomas Carmichael, William E. Lowry, and Tatiana Segura. “Hydrogel Design of Experiments Methodology to Optimize Hydrogel for iPSC-NPC Culture.” Advanced Healthcare Materials 4 (2015): 534–39.
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Siegman, Shayne, Norman F. Truong, and Tatiana Segura. “Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation.” Acta Biomaterialia 28 (2015): 45–54.
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Tokatlian, Talar, Cynthia Cam, and Tatiana Segura. “Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model.” Advanced Healthcare Materials 4 (2015): 1084–91.
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Zhu, Suwei, Lina Nih, S Thomas Carmichael, Yunfeng Lu, and Tatiana Segura. “Enzyme-Responsive Delivery of Multiple Proteins with Spatiotemporal Control.” Advanced Materials 27 (2015): 3620–25.
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Griffin, D. R., J. Borrajo, A. Soon, G. F. Acosta-Vélez, V. Oshita, N. Darling, J. MacK, T. Barker, M. L. Iruela-Arispe, and T. Segura. “Erratum: Hybrid photopatterned enzymatic reaction (HyPER) for in situ cell manipulation (ChemBioChem (2014) 15 (233-242) DOI: 10.1002/cbic.201300687).” Chembiochem 15, no. 4 (March 3, 2014): 485–86. https://doi.org/10.1002/cbic.201400067.Full Text
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Cam, Cynthia, and Tatiana Segura. “Chemical sintering generates uniform porous hyaluronic acid hydrogels.” Acta Biomaterialia 10 (2014): 205–13.
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Du, Juanjuan, Jing Jin, Yang Liu, Jie Li, Talar Tokatlian, Zuhong Lu, Tatiana Segura, Xu-bo Yuan, Xianjin Yang, and Yunfeng Lu. “Gold-nanocrystal-enhanced bioluminescent nanocapsules.” Acs Nano 8 (2014): 9964–69.
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Griffin, Donald R., Jacob Borrajo, Allyson Soon, Giovanny F. Acosta-Vèlez, Victor Oshita, Nicole Darling, Julia Mack, Thomas Barker, M Luisa Iruela-Arispe, and Tatiana Segura. “Hybrid photopatterned enzymatic reaction (HyPER) for in situ cell manipulation.” Chembiochem 15 (2014): 233–42.
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Lam, Jonathan, Bill Lowry, S Thomas Carmichael, and Tatiana Segura. “Optimizing hydrogels in vitro for transplantation of iPS-NPCs in vivo after stroke,” 2014.
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Lam, Jonathan, Norman F. Truong, and Tatiana Segura. “Design of cell–matrix interactions in hyaluronic acid hydrogel scaffolds.” Acta Biomaterialia 10 (2014): 1571–80.
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Lam, Jonathan, William E. Lowry, S Thomas Carmichael, and Tatiana Segura. “Delivery of iPS-NPCs to the Stroke Cavity within a Hyaluronic Acid Matrix Promotes the Differentiation of Transplanted Cells.” Advanced Functional Materials 24 (2014): 7053–62.
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Nowak-Sliwinska, Patrycja, Tatiana Segura, and M Luisa Iruela-Arispe. “The chicken chorioallantoic membrane model in biology, medicine and bioengineering.” Angiogenesis 17 (2014): 779–804.
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Tokatlian, Talar, Cynthia Cam, and Tatiana Segura. “Non-viral DNA delivery from porous hyaluronic acid hydrogels in mice.” Biomaterials 35 (2014): 825–35.
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Zhu, Suwei, and Tatiana Segura. “Hydrogel-based nanocomposites of therapeutic proteins for tissue repair.” Current Opinion in Chemical Engineering 4 (2014): 128–36.
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Cam, Cynthia, and Tatiana Segura. “Matrix-based gene delivery for tissue repair.” Current Opinion in Biotechnology 24 (2013): 855–63.
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Dhaliwal, Anandika, Victor Oshita, and Tatiana Segura. “Transfection in the third dimension.” Integrative Biology 5 (2013): 1206–16.
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Lam, Jonathan, and Tatiana Segura. “The modulation of MSC integrin expression by RGD presentation.” Biomaterials 34 (2013): 3938–47.
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Pannier, Angela K., and Tatiana Segura. “Surface-and hydrogel-mediated delivery of nucleic acid nanoparticles.” Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, 2013, 149–69.
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Dhaliwal, A., J. Lam, M. Maldonado, C. Lin, and T. Segura. “Extracellular matrix modulates non-viral gene transfer to mouse mesenchymal stem cells.” Soft Matter 8, no. 5 (February 7, 2012): 1451–59. https://doi.org/10.1039/c1sm06591b.Full Text
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Dhaliwal, Anandika, Maricela Maldonado, Clayton Lin, and Tatiana Segura. “Cellular cytoskeleton dynamics modulates non-viral gene delivery through RhoGTPases.” Plos One 7 (2012): e35046–e35046.
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Grover, Gregory N., Jonathan Lam, Thi H. Nguyen, Tatiana Segura, and Heather D. Maynard. “Biocompatible hydrogels by oxime Click chemistry.” Biomacromolecules 13 (2012): 3013–17.
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Tokatlian, Talar, Cynthia Cam, Shayne N. Siegman, Yuguo Lei, and Tatiana Segura. “Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs.” Acta Biomaterialia 8 (2012): 3921–31.
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Anderson, Sean M., Bhupinder Shergill, Zachary T. Barry, Eleana Manousiouthakis, Tom T. Chen, Elliot Botvinick, Manu O. Platt, M Luisa Iruela-Arispe, and Tatiana Segura. “VEGF internalization is not required for VEGFR-2 phosphorylation in bioengineered surfaces with covalently linked VEGF.” Integrative Biology 3 (2011): 887–96.
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Anderson, Sean M., Shayne N. Siegman, and Tatiana Segura. “The effect of vascular endothelial growth factor (VEGF) presentation within fibrin matrices on endothelial cell branching.” Biomaterials 32 (2011): 7432–43.
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Collier, Joel H., and Tatiana Segura. “Evolving the use of peptides as components of biomaterials.” Biomaterials 32 (2011): 4198–4204.
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Gojgini, Shiva, Talar Tokatlian, and Tatiana Segura. “Utilizing cell–matrix interactions to modulate gene transfer to stem cells inside hyaluronic acid hydrogels.” Molecular Pharmaceutics 8 (2011): 1582–91.
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Lei, Yuguo, Maha Rahim, Quinn Ng, and Tatiana Segura. “Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery.” Journal of Controlled Release 153 (2011): 255–61.
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Lei, Yuguo, Shiva Gojgini, Jonathan Lam, and Tatiana Segura. “The spreading, migration and proliferation of mouse mesenchymal stem cells cultured inside hyaluronic acid hydrogels.” Biomaterials 32 (2011): 39–47.
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Ng, Quinn K. T., Helen Su, Amanda Lee Armijo, Johannes Czernin, Caius G. Radu, and Tatiana Segura. “Clustered Arg–Gly–Asp peptides enhances tumor targeting of nonviral vectors.” Chemmedchem 6 (2011): 623–27.
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Wen, Jing, Sean M. Anderson, Juanjuan Du, Ming Yan, Jun Wang, Meiqing Shen, Yunfeng Lu, and Tatiana Segura. “Controlled Protein Delivery Based on Enzyme-Responsive Nanocapsules.” Advanced Materials 23 (2011): 4549–53.
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Zhang, Jianjun, Juanjuan Du, Ming Yan, Anandika Dhaliwal, Jing Wen, Fengqi Liu, Tatiana Segura, and Yunfeng Lu. “Synthesis of protein nano-conjugates for cancer therapy.” Nano Research 4 (2011): 425–33.
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Zhang, Jianjun, Talar Tokatlian, Jin Zhong, Quinn K. T. Ng, Michaela Patterson, William E. Lowry, S Thomas Carmichael, and Tatiana Segura. “Physically Associated Synthetic Hydrogels with Long-Term Covalent Stabilization for Cell Culture and Stem Cell Transplantation.” Advanced Materials 23 (2011): 5098–5103.
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Zhang, Jianjun, Yuguo Lei, Anandika Dhaliwal, Quinn K. T. Ng, Juanjuan Du, Ming Yan, Yunfeng Lu, and Tatiana Segura. “Protein- Polymer Nanoparticles for Nonviral Gene Delivery.” Biomacromolecules 12 (2011): 1006–14.
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Chen, Tom T., Alfonso Luque, Sunyoung Lee, Sean M. Anderson, Tatiana Segura, and M Luisa Iruela-Arispe. “Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells.” The Journal of Cell Biology 188 (2010): 595–609.
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Dhaliwal, Anandika, Maricela Maldonado, Zenas Han, and Tatiana Segura. “Differential uptake of DNA–poly (ethylenimine) polyplexes in cells cultured on collagen and fibronectin surfaces.” Acta Biomaterialia 6 (2010): 3436–47.
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Du, Juanjuan, Changming Yu, Daocheng Pan, Jianmin Li, Wei Chen, Ming Yan, Tatiana Segura, and Yunfeng Lu. “Quantum-dot-decorated robust transductable bioluminescent nanocapsules.” Journal of the American Chemical Society 132 (2010): 12780–81.
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Lei, Yuguo, Quinn K. T. Ng, and Tatiana Segura. “Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds.” Microscopy Research and Technique 73 (2010): 910–17.
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Lei, Yuguo, Suxian Huang, Pooria Sharif-Kashani, Yong Chen, Pirouz Kavehpour, and Tatiana Segura. “Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds.” Biomaterials 31 (2010): 9106–16.
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Tokatlian, Talar, Chadwick T. Shrum, Warren M. Kadoya, and Tatiana Segura. “Protease degradable tethers for controlled and cell-mediated release of nanoparticles in 2-and 3-dimensions.” Biomaterials 31 (2010): 8072–80.
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Tokatlian, Talar, and Tatiana Segura. “siRNA applications in nanomedicine.” Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 2 (2010): 305–15.
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Yan, Ming, Juanjuan Du, Zhen Gu, Min Liang, Yufang Hu, Wenjun Zhang, Saul Priceman, et al. “A novel intracellular protein delivery platform based on single-protein nanocapsules.” Nature Nanotechnology 5 (2010): 48–53.
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Anderson, Sean M., Tom T. Chen, M Luisa Iruela-Arispe, and Tatiana Segura. “The phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) by engineered surfaces with electrostatically or covalently immobilized VEGF.” Biomaterials 30 (2009): 4618–28.
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Lei, Yuguo, and Tatiana Segura. “DNA delivery from matrix metalloproteinase degradable poly (ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells.” Biomaterials 30 (2009): 254–65.
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Ng, Quinn K. T., Marie K. Sutton, Pan Soonsawad, Li Xing, Holland Cheng, and Tatiana Segura. “Engineering clustered ligand binding into nonviral vectors: αvβ3 targeting as an example.” Molecular Therapy 17 (2009): 828–36.
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Adel ddotow, Catharina, Tatiana Segura, Jeffrey A. Hubbell, and Peter Frey. “The effect of enzymatically degradable poly (ethylene glycol) hydrogels on smooth muscle cell phenotype.” Biomaterials 29 (2008): 314–26.
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Segura, Tatiana, Hugo Schmokel, and Jeffrey A. Hubbell. “RNA interference targeting hypoxia inducible factor 1α reduces post-operative adhesions in rats.” Journal of Surgical Research 141 (2007): 162–70.
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Segura, Tatiana, and Jeffrey A. Hubbell. “Synthesis and in vitro characterization of an ABC triblock copolymer for siRNA delivery.” Bioconjugate Chemistry 18 (2007): 736–45.
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Segura, Tatiana. “Formulations and Delivery Limitations of Nucleic-Acid-Based Therapies.” Handbook of Pharmaceutical Biotechnology, 2007, 1013–59.
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Segura, Tatiana. “Materials for cell-triggered and efficient gene transfer.” Nanomedicine: Nanotechnology, Biology and Medicine 3 (2007): 344–344.
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Bengali, Zain, Angela K. Pannier, Tatiana Segura, Brian C. Anderson, Jae-Hyung Jang, Thomas A. Mustoe, and Lonnie D. Shea. “Gene delivery through cell culture substrate adsorbed DNA complexes.” Biotechnology and Bioengineering 90 (2005): 290–302.
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Segura, Tatiana, Brian C. Anderson, Peter H. Chung, Rebecca E. Webber, Kenneth R. Shull, and Lonnie D. Shea. “Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern.” Biomaterials 26 (2005): 359–71.
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Segura, Tatiana, Peter H. Chung, and Lonnie D. Shea. “DNA delivery from hyaluronic acid-collagen hydrogels via a substrate-mediated approach.” Biomaterials 26 (2005): 1575–84.
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Segura, Tatiana, Matthew J. Volk, and Lonnie D. Shea. “Substrate-mediated DNA delivery: role of the cationic polymer structure and extent of modification.” Journal of Controlled Release 93 (2003): 69–84.
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Segura, Tatiana, and Lonnie D. Shea. “Surface-tethered DNA complexes for enhanced gene delivery.” Bioconjugate Chemistry 13 (2002): 621–29.
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Segura, Tatiana, and Lonnie D. Shea. “Materials for non-viral gene delivery.” Annual Review of Materials Research 31 (2001): 25–46.
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Other Articles
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Griffin, U. S. “CONTROLLABLE SELF-ANNEALING MICROGEL PARTICLES FOR BIOMEDICAL APPLICATIONS,” December 2017.
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Segura, Tatiana, and Jeffrey A. Hubbell. “Triblock copolymers for cytoplasmic delivery of gene-based drugs,” November 29, 2016.
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Griffin, Donald R., Westbrook Weaver, Tatiana Segura, Dino Di Carlo, and Philip Scumpia. “Controllable self-annealing microgel particles for biomedical applications,” September 2016.
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Lu, Yunfeng, Tatiana Segura, Suwei Zhu, and Jing Wen. “Enzyme responsive nanocapsules for protein delivery,” December 17, 2015.
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Maynard, Heather D., Tatiana Segura, Gregory Grover, and Jonathan Lam. “Method of creating hydrogels through oxime bond fomration,” July 23, 2015.
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Segura, Tatiana. “Enzyme-assisted spatial decoration of biomaterials,” April 16, 2015.
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Konstantopoulos, Konstantinos, and Tatiana Segura. “Editorial overview: Biological engineering: Advances in smart and controlled delivery of therapeutic molecules.” Elsevier, 2015.
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Segura, Tatiana, and Quinn Ng. “Multivalent clustering targeting strategy for drug carriers,” September 11, 2012.
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Segura, Tatiana, Lonnie D. Shea, Angela K. Pannier, Zain Bengali, Jae-Hyung Jang, Peter Chung, and Brian C. Anderson. “Controlled surface-associated delivery of genes and oligonucleotides.” Biological Systems Engineering: Papers and Publications, 2006.
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Segura, Tatiana, and Lonnie D. Shea. “Controlled surface-associated delivery of genes and oligonucleotides,” May 10, 2005.
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- Teaching & Mentoring
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Recent Courses
- BME 791: Graduate Independent Study 2023
- EGR 393: Research Projects in Engineering 2023
- NEUROSCI 493: Research Independent Study 1 2023
- NEUROSCI 495: Research Independent Study 3 2023
- BME 394: Projects in Biomedical Engineering (GE) 2022
- BME 493: Projects in Biomedical Engineering (GE) 2022
- BME 494: Projects in Biomedical Engineering (GE) 2022
- BME 771: Bioconjugation in drug biomaterials and drug delivery systems 2022
- BME 791: Graduate Independent Study 2022
- EGR 393: Research Projects in Engineering 2022
- NEUROSCI 493: Research Independent Study 1 2022
- NEUROSCI 494: Research Independent Study 2 2022
- BME 394: Projects in Biomedical Engineering (GE) 2021
- BME 493: Projects in Biomedical Engineering (GE) 2021
- BME 771: Bioconjugation in drug biomaterials and drug delivery systems 2021
- BME 790: Advanced Topics for Graduate Students in Biomedical Engineering 2021
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