Scholars@Duke
Che-Chia Hsu

Che-Chia Hsu

Assistant Professor of Pathology
Pathology
che-chia.hsu@duke.edu
210 Research Dr, GSRB2 Building, Room 4014, Durham, NC 27710
210 Research Drive, GSRB2 Building, Room 4014, Durham, NC 27710

Selected Publications

IMPA1-derived inositol maintains stemness in castration-resistant prostate cancer via IMPDH2 activation.

Journal Article J Exp Med · November 4, 2024 Acquisition of prostate cancer stem cells (PCSCs) manifested during androgen ablation therapy (ABT) contributes to castration-resistant prostate cancer (CRPC). However, little is known about the specific metabolites critically orchestrating this process. H ... Full text Link to item Cite

NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance.

Journal Article Cell metabolism · October 2023 Glucose metabolism is known to orchestrate oncogenesis. Whether glucose serves as a signaling molecule directly regulating oncoprotein activity for tumorigenesis remains elusive. Here, we report that glucose is a cofactor binding to methyltransferase NSUN2 ... Full text Cite

Deregulated transcription factors in cancer cell metabolisms and reprogramming.

Journal Article Seminars in cancer biology · November 2022 Metabolic reprogramming is an important cancer hallmark that plays a key role in cancer malignancies and therapy resistance. Cancer cells reprogram the metabolic pathways to generate not only energy and building blocks but also produce numerous key signali ... Full text Cite

AMPK signaling and its targeting in cancer progression and treatment.

Journal Article Seminars in cancer biology · October 2022 The intrinsic mechanisms sensing the imbalance of energy in cells are pivotal for cell survival under various environmental insults. AMP-activated protein kinase (AMPK) serves as a central guardian maintaining energy homeostasis by orchestrating diverse ce ... Full text Cite

Identification of myo-inositol-binding proteins by using the biotin pull-down strategy in cultured cells.

Journal Article STAR Protoc · June 17, 2022 Metabolites are not only substrates in metabolic reactions, but they also serve as signaling molecules to regulate diverse biological functions. Identification of the binding proteins for the metabolites helps in the understanding of their functions beyond ... Full text Open Access Link to item Cite

SIRPγ-expressing cancer stem-like cells promote immune escape of lung cancer via Hippo signaling.

Journal Article The Journal of clinical investigation · March 2022 Cancer stem-like cells (CSLCs) acquire enhanced immune checkpoint responses to evade immune cell killing and promote tumor progression. Here we showed that signal regulatory protein γ (SIRPγ) determined CSLC properties and immune evasiveness in a small pop ... Full text Open Access Cite

Inositol serves as a natural inhibitor of mitochondrial fission by directly targeting AMPK.

Journal Article Molecular cell · September 2021 Mitochondrial dynamics regulated by mitochondrial fusion and fission maintain mitochondrial functions, whose alterations underline various human diseases. Here, we show that inositol is a critical metabolite directly restricting AMPK-dependent mitochondria ... Full text Open Access Cite

Author Correction: SETDB1-mediated methylation of Akt promotes its K63-linked ubiquitination and activation leading to tumorigenesis.

Journal Article Nat Cell Biol · June 2021 A Correction to this paper has been published: https://doi.org/10.1038/s41556-021-00686-x. ... Full text Link to item Cite

The Skp2 Pathway: A Critical Target for Cancer Therapy.

Journal Article Seminars in cancer biology · December 2020 Strictly regulated protein degradation by ubiquitin-proteasome system (UPS) is essential for various cellular processes whose dysregulation is linked to serious diseases including cancer. Skp2, a well characterized component of Skp2-SCF E3 ligase complex, ... Full text Open Access Cite

Phosphorylation of PDHA by AMPK Drives TCA Cycle to Promote Cancer Metastasis.

Journal Article Molecular cell · October 2020 Cancer metastasis accounts for the major cause of cancer-related deaths. How disseminated cancer cells cope with hostile microenvironments in secondary site for full-blown metastasis is largely unknown. Here, we show that AMPK (AMP-activated protein kinase ... Full text Cite

Increased activation of HDAC1/2/6 and Sp1 underlies therapeutic resistance and tumor growth in glioblastoma.

Journal Article Neuro-oncology · October 2020 BackgroundGlioblastoma is associated with poor prognosis and high mortality. Although the use of first-line temozolomide can reduce tumor growth, therapy-induced stress drives stem cells out of quiescence, leading to chemoresistance and glioblasto ... Full text Cite

Lactate Is a Natural Suppressor of RLR Signaling by Targeting MAVS.

Journal Article Cell · June 2019 RLR-mediated type I IFN production plays a pivotal role in elevating host immunity for viral clearance and cancer immune surveillance. Here, we report that glycolysis, which is inactivated during RLR activation, serves as a barrier to impede type I IFN pro ... Full text Open Access Cite

SETDB1-mediated methylation of Akt promotes its K63-linked ubiquitination and activation leading to tumorigenesis.

Journal Article Nature cell biology · February 2019 The serine/threonine kinase Akt plays a central role in cell proliferation, survival and metabolism, and its hyperactivation is linked to cancer progression. Here we report that Akt undergoes K64 methylation by SETDB1, which is crucial for cell membrane re ... Full text Cite

Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.

Journal Article Biochem Biophys Res Commun · November 4, 2017 It has been suggested that stress stimuli from the microenvironment maintain a subset of tumor cells with stem-like properties, including drug resistance. Here, we investigate whether Sp1, a stress-responsive factor, regulates stemness gene expression and ... Full text Link to item Cite

Specificity protein 1-modulated superoxide dismutase 2 enhances temozolomide resistance in glioblastoma, which is independent of O6-methylguanine-DNA methyltransferase.

Journal Article Redox Biol · October 2017 Acquisition of temozolomide (TMZ) resistance is a major factor leading to the failure of glioblastoma (GBM) treatment. The exact mechanism by which GBM evades TMZ toxicity is not always related to the expression of the DNA repair enzyme O6-methylguanine-DN ... Full text Link to item Cite

Suberoylanilide hydroxamic acid represses glioma stem-like cells.

Journal Article J Biomed Sci · November 18, 2016 BACKGROUND: Glioma stem-like cells (GSCs) are proposed to be responsible for high resistance in glioblastoma multiforme (GBM) treatment. In order to find new strategies aimed at reducing GSC stemness and improving GBM patient survival, we investigated the ... Full text Link to item Cite

RINT-1 interacts with MSP58 within nucleoli and plays a role in ribosomal gene transcription.

Journal Article Biochem Biophys Res Commun · September 16, 2016 The nucleolus is the cellular site of ribosomal (r)DNA transcription and ribosome biogenesis. The 58-kDa microspherule protein (MSP58) is a nucleolar protein involved in rDNA transcription and cell proliferation. However, regulation of MSP58-mediated rDNA ... Full text Link to item Cite

The 58-kda microspherule protein (MSP58) represses human telomerase reverse transcriptase (hTERT) gene expression and cell proliferation by interacting with telomerase transcriptional element-interacting factor (TEIF).

Journal Article Biochim Biophys Acta · March 2014 58-kDa microspherule protein (MSP58) plays an important role in a variety of cellular processes including transcriptional regulation, cell proliferation and oncogenic transformation. Currently, the mechanisms underlying the oncogenic effect of MSP58 are no ... Full text Link to item Cite

58-kDa microspherule protein (MSP58) is novel Brahma-related gene 1 (BRG1)-associated protein that modulates p53/p21 senescence pathway.

Journal Article The Journal of biological chemistry · June 2012 The nucleolar 58-kDa microspherule protein (MSP58) protein is a candidate oncogene implicated in modulating cellular proliferation and malignant transformation. In this study, we show that knocking down MSP58 expression caused aneuploidy and led to apoptos ... Full text Cite