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Hongtao Michael Guo

Associate Professor of Orthopaedic Surgery
Orthopaedic Surgery
Box 3077, DUMC, Durham, NC 27710
200 Trent Drive, Durham, NC 27710

Selected Publications


Vaccination targeting human HER3 alters the phenotype of infiltrating T cells and responses to immune checkpoint inhibition.

Journal Article Oncoimmunology · 2017 Expression of human epidermal growth factor family member 3 (HER3), a critical heterodimerization partner with EGFR and HER2, promotes more aggressive biology in breast and other epithelial malignancies. As such, inhibiting HER3 could have broad applicabil ... Full text Link to item Cite

Precision cancer immunotherapy: optimizing dendritic cell-based strategies to induce tumor antigen-specific T-cell responses against individual patient tumors.

Journal Article J Immunother · May 2015 Most dendritic cell (DC)-based vaccines have loaded the DC with defined antigens, but loading with autologos tumor-derived antigens would generate DCs that activate personalized tumor-specific T-cell responses. We hypothesized that DC matured with an optim ... Full text Link to item Cite

Human mesenchymal stem cell and epithelial hepatic carcinoma cell lines in admixture: concurrent stimulation of cancer-associated fibroblasts and epithelial-to-mesenchymal transition markers.

Journal Article Surgery · September 2012 BACKGROUND: The microenvironments of neoplasms influence both mesenchymal stem cell differentiation into cancer-associated fibroblasts (CAF) and tumor cell line differentiation to mesenchymal phenotypes via epithelial-to-mesenchymal transition (EMT). Using ... Full text Link to item Cite

Osteopontin regulates epithelial mesenchymal transition-associated growth of hepatocellular cancer in a mouse xenograft model.

Journal Article Ann Surg · February 2012 OBJECTIVE: To determine the efficacy of osteopontin (OPN) targeting in hepatocellular cancer (HCC). SUMMARY/BACKGROUND: OPN is associated with HCC growth and metastasis and represents a unique therapeutic target. METHODS: OPN and epithelial-mesenchymal tra ... Full text Link to item Cite

Increasing vaccine potency through exosome antigen targeting.

Journal Article Vaccine · November 21, 2011 While many tumor associated antigens (TAAs) have been identified in human cancers, efforts to develop efficient TAA "cancer vaccines" using classical vaccine approaches have been largely ineffective. Recently, a process to specifically target proteins to e ... Full text Link to item Cite

Pharmacokinetic characterization of an RNA aptamer against osteopontin and demonstration of in vivo efficacy in reversing growth of human breast cancer cells.

Journal Article Surgery · August 2011 BACKGROUND: We report pharmacokinetic (PK) data, evaluation of modifications for increased stability, evaluation for cellular uptake, and mediation of regression of breast cancer for the aptamer OPN-R3. METHODS: The OPN-R3 aptamer was assessed for PK data ... Full text Link to item Cite

Osteopontin promotes CCL5-mesenchymal stromal cell-mediated breast cancer metastasis.

Journal Article Carcinogenesis · April 2011 The interaction between cancer and its local microenvironment can determine properties of growth and metastasis. A critical component of the tumor microenvironment in this context is the cancer-associated fibroblast (CAF), which can promote tumor growth, a ... Full text Link to item Cite

Osteopontin and protein kinase C regulate PDLIM2 activation and STAT1 ubiquitination in LPS-treated murine macrophages.

Journal Article J Biol Chem · November 26, 2010 The molecular pathways regulating signal transducer and activator of transcription 1 (STAT1) levels in states of inflammation are incompletely understood. The suppressor of cytokine signaling, protein inhibitor of STAT, and SHP-1/2 tyrosine phosphatases ul ... Full text Link to item Cite

Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines.

Journal Article Surgery · August 2010 BACKGROUND: Osteopontin (OPN) is a variably expressed, secreted glycophosphoprotein that mediates the growth and metastases of hepatocellular cancer (HCC). MicroRNAs (miRNAs) may be responsible for variant OPN expression, interrupting translation by bindin ... Full text Link to item Cite

Identification of osteopontin-dependent signaling pathways in a mouse model of human breast cancer.

Journal Article BMC Res Notes · July 1, 2009 BACKGROUND: Osteopontin (OPN) is a secreted phosphoprotein which functions as a cell attachment protein and cytokine that signals through two cell adhesion molecules, alphavbeta3-integrin and CD44, to regulate cancer growth and metastasis. However, the sig ... Full text Link to item Cite

EF1A1-actin interactions alter mRNA stability to determine differential osteopontin expression in HepG2 and Hep3B cells.

Journal Article Exp Cell Res · January 15, 2009 Cancer progression depends on an accumulation of metastasis-supporting physiological changes which are regulated by cell signaling molecules. One such molecule, osteopontin (OPN), is a secreted phosphoprotein which mediates increased cellular migratory and ... Full text Link to item Cite

RNA aptamer blockade of osteopontin inhibits growth and metastasis of MDA-MB231 breast cancer cells.

Journal Article Mol Ther · January 2009 Osteopontin (OPN) is a secreted phosphoprotein which mediates tumorigenesis, local growth, and metastasis in a variety of cancers. It is a potential therapeutic target for the regulation of cancer metastasis. RNA aptamer technology targeting OPN may repres ... Full text Link to item Cite

Characterization of short range DNA looping in endotoxin-mediated transcription of the murine inducible nitric-oxide synthase (iNOS) gene.

Journal Article J Biol Chem · September 12, 2008 The local structural properties and spatial conformations of chromosomes are intimately associated with gene expression. The spatial associations of critical genomic elements in inducible nitric-oxide synthase (iNOS) transcription have not been previously ... Full text Link to item Cite

Osteopontin mediates Stat1 degradation to inhibit iNOS transcription in a cecal ligation and puncture model of sepsis.

Journal Article Surgery · August 2008 BACKGROUND: Osteopontin (OPN) represses inducible nitric oxide synthase (iNOS) expression by increasing ubiquitin (Ub)-proteasome degradation of Stat1, a critical transcription factor for iNOS expression. We investigated the in vivo relevance of our findin ... Full text Link to item Cite

Vaccination targeting human HER3 alters the phenotype of infiltrating T cells and responses to immune checkpoint inhibition.

Journal Article Oncoimmunology · 2017 Expression of human epidermal growth factor family member 3 (HER3), a critical heterodimerization partner with EGFR and HER2, promotes more aggressive biology in breast and other epithelial malignancies. As such, inhibiting HER3 could have broad applicabil ... Full text Link to item Cite

Precision cancer immunotherapy: optimizing dendritic cell-based strategies to induce tumor antigen-specific T-cell responses against individual patient tumors.

Journal Article J Immunother · May 2015 Most dendritic cell (DC)-based vaccines have loaded the DC with defined antigens, but loading with autologos tumor-derived antigens would generate DCs that activate personalized tumor-specific T-cell responses. We hypothesized that DC matured with an optim ... Full text Link to item Cite

Human mesenchymal stem cell and epithelial hepatic carcinoma cell lines in admixture: concurrent stimulation of cancer-associated fibroblasts and epithelial-to-mesenchymal transition markers.

Journal Article Surgery · September 2012 BACKGROUND: The microenvironments of neoplasms influence both mesenchymal stem cell differentiation into cancer-associated fibroblasts (CAF) and tumor cell line differentiation to mesenchymal phenotypes via epithelial-to-mesenchymal transition (EMT). Using ... Full text Link to item Cite

Osteopontin regulates epithelial mesenchymal transition-associated growth of hepatocellular cancer in a mouse xenograft model.

Journal Article Ann Surg · February 2012 OBJECTIVE: To determine the efficacy of osteopontin (OPN) targeting in hepatocellular cancer (HCC). SUMMARY/BACKGROUND: OPN is associated with HCC growth and metastasis and represents a unique therapeutic target. METHODS: OPN and epithelial-mesenchymal tra ... Full text Link to item Cite

Increasing vaccine potency through exosome antigen targeting.

Journal Article Vaccine · November 21, 2011 While many tumor associated antigens (TAAs) have been identified in human cancers, efforts to develop efficient TAA "cancer vaccines" using classical vaccine approaches have been largely ineffective. Recently, a process to specifically target proteins to e ... Full text Link to item Cite

Pharmacokinetic characterization of an RNA aptamer against osteopontin and demonstration of in vivo efficacy in reversing growth of human breast cancer cells.

Journal Article Surgery · August 2011 BACKGROUND: We report pharmacokinetic (PK) data, evaluation of modifications for increased stability, evaluation for cellular uptake, and mediation of regression of breast cancer for the aptamer OPN-R3. METHODS: The OPN-R3 aptamer was assessed for PK data ... Full text Link to item Cite

Osteopontin promotes CCL5-mesenchymal stromal cell-mediated breast cancer metastasis.

Journal Article Carcinogenesis · April 2011 The interaction between cancer and its local microenvironment can determine properties of growth and metastasis. A critical component of the tumor microenvironment in this context is the cancer-associated fibroblast (CAF), which can promote tumor growth, a ... Full text Link to item Cite

Osteopontin and protein kinase C regulate PDLIM2 activation and STAT1 ubiquitination in LPS-treated murine macrophages.

Journal Article J Biol Chem · November 26, 2010 The molecular pathways regulating signal transducer and activator of transcription 1 (STAT1) levels in states of inflammation are incompletely understood. The suppressor of cytokine signaling, protein inhibitor of STAT, and SHP-1/2 tyrosine phosphatases ul ... Full text Link to item Cite

Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines.

Journal Article Surgery · August 2010 BACKGROUND: Osteopontin (OPN) is a variably expressed, secreted glycophosphoprotein that mediates the growth and metastases of hepatocellular cancer (HCC). MicroRNAs (miRNAs) may be responsible for variant OPN expression, interrupting translation by bindin ... Full text Link to item Cite

Identification of osteopontin-dependent signaling pathways in a mouse model of human breast cancer.

Journal Article BMC Res Notes · July 1, 2009 BACKGROUND: Osteopontin (OPN) is a secreted phosphoprotein which functions as a cell attachment protein and cytokine that signals through two cell adhesion molecules, alphavbeta3-integrin and CD44, to regulate cancer growth and metastasis. However, the sig ... Full text Link to item Cite

EF1A1-actin interactions alter mRNA stability to determine differential osteopontin expression in HepG2 and Hep3B cells.

Journal Article Exp Cell Res · January 15, 2009 Cancer progression depends on an accumulation of metastasis-supporting physiological changes which are regulated by cell signaling molecules. One such molecule, osteopontin (OPN), is a secreted phosphoprotein which mediates increased cellular migratory and ... Full text Link to item Cite

RNA aptamer blockade of osteopontin inhibits growth and metastasis of MDA-MB231 breast cancer cells.

Journal Article Mol Ther · January 2009 Osteopontin (OPN) is a secreted phosphoprotein which mediates tumorigenesis, local growth, and metastasis in a variety of cancers. It is a potential therapeutic target for the regulation of cancer metastasis. RNA aptamer technology targeting OPN may repres ... Full text Link to item Cite

Characterization of short range DNA looping in endotoxin-mediated transcription of the murine inducible nitric-oxide synthase (iNOS) gene.

Journal Article J Biol Chem · September 12, 2008 The local structural properties and spatial conformations of chromosomes are intimately associated with gene expression. The spatial associations of critical genomic elements in inducible nitric-oxide synthase (iNOS) transcription have not been previously ... Full text Link to item Cite

Osteopontin mediates Stat1 degradation to inhibit iNOS transcription in a cecal ligation and puncture model of sepsis.

Journal Article Surgery · August 2008 BACKGROUND: Osteopontin (OPN) represses inducible nitric oxide synthase (iNOS) expression by increasing ubiquitin (Ub)-proteasome degradation of Stat1, a critical transcription factor for iNOS expression. We investigated the in vivo relevance of our findin ... Full text Link to item Cite

RNA stability regulates differential expression of the metastasis protein, osteopontin, in hepatocellular cancer.

Journal Article Surgery · June 2008 BACKGROUND: Osteopontin (OPN) is a potential therapeutic target in hepatocellular carcinoma (HCC), because it is a critical mediator of metastatic function. The molecular mechanisms that determine expression of OPN in HCC, however, are unknown. In this stu ... Full text Link to item Cite

Osteopontin regulates ubiquitin-dependent degradation of Stat1 in murine mammary epithelial tumor cells.

Journal Article Neoplasia · September 2007 BACKGROUND: Osteopontin (OPN) is a secreted glycoprotein that mediates cell-matrix interactions and cellular signaling by binding with integrin (primarily alpha(v)beta(3)) and CD44 receptors. OPN regulates cell adhesion, chemotaxis, macrophage-directed IL- ... Full text Link to item Cite

Sp1 regulates osteopontin expression in SW480 human colon adenocarcinoma cells.

Journal Article Surgery · August 2007 BACKGROUND: Osteopontin (OPN) mediates cancer metastasis. Mechanisms regulating OPN expression in human colorectal cancer are unknown. Using SW480 colon adenocarcinoma cells, we hypothesized that transcription determines OPN expression. METHODS: SW480 cons ... Full text Link to item Cite

Stat1 acetylation inhibits inducible nitric oxide synthase expression in interferon-gamma-treated RAW264.7 murine macrophages.

Journal Article Surgery · August 2007 BACKGROUND: We hypothesized that acetylation of the Stat1 regulates interferon-gamma (IFN-gamma) mediated macrophage expression of inducible nitric oxide synthase (iNOS). METHODS: RAW 264.7 iNOS expression was induced with IFN-gamma. Deacetylase inhibitors ... Full text Link to item Cite

Thrombin-cleaved COOH(-) terminal osteopontin peptide binds with cyclophilin C to CD147 in murine breast cancer cells.

Journal Article Cancer Res · May 1, 2007 Osteopontin is a glycoprotein that has been linked to metastatic function in breast, lung, and prostate cancers. However, the mechanism by which osteopontin acts to induce metastatic properties is largely unknown. One intriguing feature of osteopontin is t ... Full text Link to item Cite

Characterization of the PC4 binding domain and its interactions with HNF4alpha.

Journal Article J Biochem · May 2007 In the presence of oxidative stress, the hepatocellular inflammatory-redox (IR) state upregulates inducible nitric oxide synthase (iNOS) expression as an anti-oxidant function. In IL-1beta and peroxide treated hepatocytes, we have identified hepatocyte nuc ... Full text Link to item Cite

Osteopontin induces ubiquitin-dependent degradation of STAT1 in RAW264.7 murine macrophages.

Journal Article J Immunol · February 1, 2007 In systemic inflammation induced by endotoxin (LPS), the macrophage produces the majority of the circulating NO metabolites. However, while the molecular pathways which up-regulate iNOS expression have been extensively studied in the macrophage, little is ... Full text Link to item Cite

Osteopontin inhibits macrophage nitric oxide synthesis to enhance tumor proliferation.

Journal Article Surgery · August 2006 BACKGROUND: Interactions between tumor cells and their host environment can play a major role in regulating survival programs required for tumor progression. Osteopontin (OPN) is a glycophosphoprotein overexpressed by tumors, and is a key molecule for tumo ... Full text Link to item Cite

Ets-1 and runx2 regulate transcription of a metastatic gene, osteopontin, in murine colorectal cancer cells.

Journal Article J Biol Chem · July 14, 2006 Osteopontin (OPN) is a sialic acid-rich phosphoprotein secreted by a wide variety of cancers. We have shown previously that OPN is necessary for mediating hepatic metastasis in CT26 colorectal cancer cells. Although a variety of stimuli can induce OPN, the ... Full text Link to item Cite

Integrin-linked kinase regulates osteopontin-dependent MMP-2 and uPA expression to convey metastatic function in murine mammary epithelial cancer cells.

Journal Article Carcinogenesis · June 2006 Metastasis-supporting physiological alterations are regulated by cell signaling molecules, which target signal transduction pathways and gene expression. Osteopontin (OPN) overexpression may represent a key molecular event in cancer metastasis. In this stu ... Full text Link to item Cite

Phosphorylation of Ser158 regulates inflammatory redox-dependent hepatocyte nuclear factor-4alpha transcriptional activity.

Journal Article Biochem J · March 1, 2006 In IL-1beta (interleukin 1beta)-stimulated rat hepatocytes exposed to superoxide, we have previously identified an IRX (inflammatory redox)-sensitive DR1 [direct repeat of RG(G/T)TCA with one base spacing] cis-acting activator element (nt -1327 to -1315) i ... Full text Link to item Cite

Redox-mediated upregulation of hepatocyte iNOS transcription requires coactivator PC4.

Journal Article Surgery · July 2005 BACKGROUND: Redox-mediated upregulation of transcription of hepatocyte inducible nitric oxide synthase (iNOS) requires hepatocyte nuclear factor IV-alpha (HNF-4alpha). In this setting, PC4 is often isolated with HNF-4alpha in DNA-protein pull-down studies. ... Full text Link to item Cite

Transcriptional regulatory functions of heterogeneous nuclear ribonucleoprotein-U and -A/B in endotoxin-mediated macrophage expression of osteopontin.

Journal Article J Immunol · July 1, 2005 Osteopontin (OPN) is a highly hydrophilic and negatively charged sialoprotein of approximately 298 amino acids with diverse regulatory functions, including cell adhesion and migration, tumor growth and metastasis, atherosclerosis, aortic valve calcificatio ... Full text Link to item Cite

Nitric oxide-dependent osteopontin expression induces metastatic behavior in HepG2 cells.

Journal Article Dig Dis Sci · July 2005 Our objective was to delineate the role of nitric oxide (NO) in osteopontin (OPN)-associated metastatic properties in HepG2 cells. OPN is the major phosphoprotein secreted by malignant cells in patients with advanced metastatic cancer, is frequently overex ... Full text Link to item Cite

Osteopontin silencing by small interfering RNA suppresses in vitro and in vivo CT26 murine colon adenocarcinoma metastasis.

Journal Article Carcinogenesis · April 2005 Hepatic metastasis is a primary cause for failure of locoregional therapy in colorectal cancer. Increased expression of osteopontin (OPN), a ligand for alpha(v)beta3 integrin and CD44 receptors, is associated with metastasis in several types of cancer. How ... Full text Link to item Cite

Identification of S-nitrosylated proteins in endotoxin-stimulated RAW264.7 murine macrophages.

Journal Article Nitric Oxide · March 2005 Nitric oxide (NO) is an omnipresent regulator of cell function in a variety of physiologic and pathophysiologic states. In part, NO exerts its actions by S-nitrosylation of target thiols, primarily in cysteine residues. Delineating the functional correlate ... Full text Link to item Cite

Differential osteopontin expression in phenotypically distinct subclones of murine breast cancer cells mediates metastatic behavior.

Journal Article J Biol Chem · November 5, 2004 Cancer progression depends on an accumulation of metastasis-supporting cell signaling molecules, which target signal transduction pathways and, ultimately, gene expression. One such molecule, osteopontin (OPN), represents a key molecular signaling event in ... Full text Link to item Cite

A transcriptional repressor of osteopontin expression in the 4T1 murine breast cancer cell line.

Journal Article Biochem Biophys Res Commun · September 3, 2004 Osteopontin (OPN) is a highly hydrophilic and negatively charged sialoprotein of approximately 298 amino acids which is an important mediator of tumor metastatic behavior. We have previously demonstrated that endotoxin-dependent OPN gene transcription is r ... Full text Link to item Cite

Osteopontin increases CD44 expression and cell adhesion in RAW 264.7 murine leukemia cells.

Journal Article Immunol Lett · August 15, 2004 BACKGROUND: Osteopontin (OPN) is an inducible cell attachment protein which binds alphavbeta3-integrin and CD44 receptors to promote tumor metastasis. We hypothesized that OPN alters expression of its CD44 receptor to promote neoplastic cell migration. MET ... Full text Link to item Cite

S-nitrosylation of heterogeneous nuclear ribonucleoprotein A/B regulates osteopontin transcription in endotoxin-stimulated murine macrophages.

Journal Article J Biol Chem · March 19, 2004 Osteopontin (OPN) is a highly hydrophilic and negatively charged sialoprotein of approximately 298 amino acids that contains a Gly-Arg-Gly-Asp-Ser sequence. It is a secreted protein with diverse regulatory functions, including cell adhesion and migration, ... Full text Link to item Cite

Peroxide-mediated chromatin remodelling of a nuclear factor kappa B site in the mouse inducible nitric oxide synthase promoter.

Journal Article Biochem J · February 1, 2004 Hepatocyte expression of iNOS (inducible nitric oxide synthase) and synthesis of nitric oxide convey protective antioxidant functions in models of sepsis, shock and reperfusion. However, the underlying redox-sensitive mechanisms that regulate hepatocyte ex ... Full text Link to item Cite

Osteopontin-dependent CD44v6 expression and cell adhesion in HepG2 cells.

Journal Article Carcinogenesis · December 2003 The interaction of osteopontin (OPN) with CD44 and alphavbeta3-integrin has been implicated in numerous signal transduction pathways that may promote cancer metastasis. CD44v6 is a splice variant of CD44 which has been identified as a marker of cancer prog ... Full text Link to item Cite

Osteopontin inhibits expression of cytochrome c oxidase in RAW 264.7 murine macrophages.

Journal Article Biochem Biophys Res Commun · September 12, 2003 Osteopontin (OPN) functions as both a cell attachment protein and a cytokine that signals through two CAM molecules: alpha(v)beta(3)-integrin and CD44. OPN initiates a number of signal transduction pathways that control cell survival, proliferation, and mi ... Full text Link to item Cite

Serine/threonine phosphorylation regulates HNF-4alpha-dependent redox-mediated iNOS expression in hepatocytes.

Journal Article Am J Physiol Cell Physiol · April 2003 Nitric oxide (NO), endogenously synthesized by inducible NO synthase (iNOS), serves antioxidant and antiapoptotic functions in settings characterized by oxidative stress and proinflammatory cytokines such as sepsis and shock. However, the redox-sensitive m ... Full text Link to item Cite

CDK5 regulates cell adhesion and migration in corneal epithelial cells.

Journal Article Mol Cancer Res · November 2002 CDK5 and its activator, p35, are expressed in mouse corneal epithelium and can be coimmunoprecipited from corneal epithelial cell lysates. Immunostaining shows CDK5 and p35 in all layers of the corneal epithelium, especially along the basal side of the bas ... Link to item Cite

Endotoxin-stimulated nitric oxide production inhibits expression of cytochrome c oxidase in ANA-1 murine macrophages.

Journal Article J Immunol · May 1, 2002 In endotoxin (LPS)-mediated states of sepsis, inducible NO synthase expression and NO production are associated with molecular regulatory functions that determine the host inflammatory response. NO inhibits cellular respiration and mitochondrial electron t ... Full text Link to item Cite

Hepatocyte nuclear factor-4alpha mediates redox sensitivity of inducible nitric-oxide synthase gene transcription.

Journal Article J Biol Chem · February 15, 2002 The underlying redox-sensitive mechanisms that regulate hepatocyte expression of inducible nitric-oxide synthase (iNOS) and its antioxidant functions are largely unknown. We have demonstrated previously that oxidative stress induced by benzenetriol-mediate ... Full text Link to item Cite

Nitric oxide is necessary for CC-class chemokine expression in endotoxin-stimulated ANA-1 murine macrophages.

Journal Article Immunol Lett · January 1, 2002 The production of nitric oxide (NO) in response to endotoxin (LPS)-stimulation is associated with a myriad of NO-dependent regulatory functions. The study of NO-dependent genetic programs in the setting of endotoxin stimulation can be aided by determinatio ... Full text Link to item Cite

Nitric oxide inhibits expression of cytochrome B in endotoxin-stimulated murine macrophages.

Journal Article Biochem Biophys Res Commun · December 21, 2001 In LPS-mediated states of sepsis, inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production inhibit cellular respiration and mitochondrial electron transport. NO has been demonstrated to inhibit mitochondrial respiration by nitrosy ... Full text Link to item Cite

Surgical treatment of malignant esophageal tumors in PUMC Hospital.

Journal Article Chin Med Sci J · December 2001 To study how to prolong the postoperative survival time of the patients with malignant esophageal tumors. The clinical data of 1098 patients with malignant esophageal tumors from 1961 to 1992 were retrospectively analyzed. The deletion of fragile histamine ... Link to item Cite

Cdk5 mediates changes in morphology and promotes apoptosis of astrocytoma cells in response to heat shock.

Journal Article J Cell Sci · March 2001 The cyclin-dependent kinase member, Cdk5, is expressed in a variety of cell types, but neuron-specific expression of its activator, p35, is thought to limit its activity to neurons. Here we demonstrate that both Cdk5 and p35 are expressed in the human astr ... Full text Link to item Cite

Osteopontin is a negative feedback regulator of nitric oxide synthesis in murine macrophages.

Journal Article J Immunol · January 15, 2001 In a system of endotoxin (LPS)-mediated NO production in ANA-1 murine macrophages, suppression subtractive hybridization was used to identify genes up-regulated by NO. Osteopontin (OPN), a secreted acidic phosphoprotein that binds to a cell surface RGD int ... Full text Link to item Cite

[Surgical treatment of malignant esophageal tumors in PUMC Hospital].

Journal Article Zhongguo Yi Xue Ke Xue Yuan Xue Bao · December 2000 OBJECTIVE: To study how to prolong the postoperative survival time of the patients with malignant esophageal tumors. METHODS: The clinical data of 1,098 patients with malignant esophageal tumors from 1961 to 1992 were retrospectively analyzed. The deletion ... Link to item Cite

Endotoxin and NO induce MIP-1gamma gene transcription in ANA-1 murine macrophages.

Journal Article Biochem Biophys Res Commun · November 2, 2000 The host response to gram-negative endotoxin is characterized by an influx of inflammatory cells into host tissues, mediated in part by localized production of chemokines. In this study, using subtractive suppression hybridization analysis, we demonstrate ... Full text Link to item Cite

Nitric oxide-dependent ribosomal RNA cleavage is associated with inhibition of ribosomal peptidyl transferase activity in ANA-1 murine macrophages.

Journal Article J Immunol · October 1, 2000 NO can regulate specific cellular functions by altering transcriptional programs and protein reactivity. With respect to global cellular processes, NO has also been demonstrated to inhibit total protein synthesis and cell proliferation. The underlying mech ... Full text Link to item Cite

[An epidemiologic study of irritable bowel syndrome in Beijing - a stratified randomized study by clustering sampling].

Journal Article Zhonghua Liu Xing Bing Xue Za Zhi · February 2000 OBJECTIVE: To explore the prevalence of irritable bowel syndrome (IBS) and its risk factors in Beijing. METHODS: Phase I: a screening program for IBS was carried out in Beijing area according to symptoms using both Manning (modified including constipation) ... Link to item Cite

[Murine Doube Minute 2 oncogene inhibited apoptosis induced by retroviral-mediated wild-type p53].

Journal Article Zhongguo Yi Xue Ke Xue Yuan Xue Bao · December 1998 OBJECTIVE: To investigate the interaction between Murine Doube Minute 2 (MDM2) oncogene and p53 gene in the evolution of apoptosis in pancreatic carcinoma. METHODS: A recombinant retroviral vector expressing wild-type p53 was constructed and packaged by pa ... Link to item Cite

Inhibiting effect of murine double minute-2 oncogene on apoptosis induced by retroviral-mediated wild-type p53.

Journal Article Chin Med J (Engl) · December 1998 OBJECTIVE: To investigate the effects of wild-type p53 (wtp53) on inducing apoptosis by restoring wtp53 expression in pancreatic adenocarcinoma cell line (PC-2) which contains mutant p53, and the interaction between murine double minute-2 (MDM2) and wtp53 ... Link to item Cite

[The effect of adenovirus-mediated wild-type p53 expression on the growth of human pancreatic carcinoma cell line cells].

Journal Article Zhongguo Yi Xue Ke Xue Yuan Xue Bao · October 1998 OBJECTIVE: To evaluate the effect of the wild-type p53(wtp53) expression on the growth of pancreatic carcinoma cell line. METHODS: A recombinant adenovirus vector (E1 minus)-Ad5CMVwtp53 expressing human wild-type p53 was constructed, the vector contained a ... Link to item Cite

[Apoptosis induced by adenovirus-mediated wild-type p53 expression in human pancreatic cancer cells].

Journal Article Zhonghua Bing Li Xue Za Zhi · June 1998 OBJECTIVE: To investigate the biological effects of wtp53 expression on the growth rate and apoptosis of human pancreatic carcinoma. METHODS: The Ad5CMVwtp53 a recombinant replication-deficient adenoviral vector containing a human CMV promoter, a human wil ... Link to item Cite

Diagnosis and treatment of stage-III peripheral squamous cell carcinoma of right upper lung.

Journal Article Chin Med Sci J · December 1997 During a 12-year period, 44 patients were surgically treated for stage-III peripheral squamous cell carcinoma of the right upper lobe. The mean time from the discovery of the shadow in the right upper lobe to the confirmation of the diagnosis was 2 months. ... Link to item Cite

"Smart" co-drugs. Rapid intravitreal delivery of 5-FU and prolonged delivery of steroid from the same erodible device

Conference Investigative Ophthalmology and Visual Science · December 1, 1997 Purpose: The aim of this work was to develop a small implantahle hioerodihle device giving sustained release of 5-FU for 1-2 weeks and sustained release of a steroid for over 2 months. Previous work had indicated that this may be optimal for the treatment ... Cite

[The inhibitory effect of combined treatment of TNF alpha and antisense oligodeoxynucleotides on the growth of human pancreatic carcinoma cell line cells].

Journal Article Zhonghua Bing Li Xue Za Zhi · October 1997 OBJECTIVE: To study the inhibitory effect induced by combined treatment of TNF alpha and antisense oligodeoxynucleotide on the growth of human pancreatic adenocarcinoma cell line (PC-2) cells. METHOD: TNF alpha and synthesized antisense c-myc or Ki-ras pho ... Link to item Cite

[Systemic manifestations of myasthenia gravis and its putative pathogenesis].

Journal Article Zhonghua Nei Ke Za Zhi · June 1997 We studied the systemic manifestations of myasthenia gravis and its putative pathogenesis. 644 clinically, pharmacologically and electrophysiologically diagnosed patients with myasthenia gravis (MG) were studied. 33 patients had Graves disease, 5 periodic ... Link to item Cite

Oxidative stress mediates monocrotaline-induced alterations in tenascin expression in pulmonary artery endothelial cells.

Journal Article Int J Biochem Cell Biol · May 1997 Oxidative stress may be involved in monocrotaline (MCT)-induced endothelial cell injury and upregulation of extracellular matrix proteins in the pulmonary vasculature. To test this hypothesis, cytotoxicity, expression and distribution of tenascin (TN) as w ... Full text Link to item Cite

Multiple polyamine regulatory pathways control compensatory cardiovascular hypertrophy in coarctation hypertension.

Journal Article Clin Exp Hypertens · April 1997 While a number of factors may initiate structural alterations within the cardiovascular system in response to hypertension, there are obligate cellular signaling mechanisms, such as the polyamines, through which they must operate. This study examined the e ... Full text Link to item Cite

Ion-paired codrug for increased ocular absorption

Journal Article Proceedings of the Controlled Release Society · January 1, 1997 Cite

[Studies on MDM2 oncogene expression and its effect on pancreatic carcinoma cells].

Journal Article Zhonghua Bing Li Xue Za Zhi · August 1996 In order to study the interrelation and interaction between MDM2 oncogene and wild type p53 in human pancreatic cancer, we studied the expression and amplification of MDM2 oncogene and its antagonistic effect on wild type p53 by use of gene recombination, ... Link to item Cite

Sustained release ciprofloxacin and diclofenac from a disposable contact lens

Journal Article Investigative Ophthalmology and Visual Science · February 15, 1996 Purpose: To develop contact lens giving 24 hour sustained release of an anti-inflammatory (diclofenac) and an anti-biotic (ciprofloxacin). Methods 1) Preliminary Work: Solutions of sodium diclofenac were prepared and added to solutions of ciprofloxacin HCl ... Cite

Antibiotic/nonsteroidal anti-inflammatory codrugs for topical application

Journal Article Investigative Ophthalmology and Visual Science · February 15, 1996 Purpose: This study investigated ion-paired codrug suspensions tobramycin with diclofenac as a means to achieve increased residence time and ocular absorption after topical application. Methods: Solutions of sodium diclofenac were prepared and added to sol ... Cite

Efficacy and pharmacokinetics of intravitreal sustained release triamcinolone/5-FU codrug suspension in the treatment of experimental PVR

Conference Investigative Ophthalmology and Visual Science · February 15, 1996 Purpose. Last year we described a novel method of delivering a triamcinolone acetonide (TA)/5-FU conjugate as a sustained release suspension for the treatment of PVR in a fibroblast -injection model. Herein, we evaluate the pharmacokinetics and efficacy of ... Cite

Oxidized lipid-mediated alterations in proteoglycan metabolism in cultured pulmonary endothelial cells.

Journal Article Atherosclerosis · February 1996 Compared to cholesterol or linoleic acid (18:2), oxidized lipids such as cholestan-3 beta, 5 alpha, 6 beta-triol (triol) and hydroperoxy linoleic acid (HPODE) markedly impair endothelial barrier function in culture [Hennig and Boissonneault, 1987; Hennig e ... Full text Link to item Cite

Disease enhanced delivery from erodible implants

Conference Proceedings of the Controlled Release Society · January 1, 1995 Cite

Sustained release of synergistic agents

Conference Proceedings of the Controlled Release Society · January 1, 1995 Cite

Anisodamine at higher concentrations in inhibiting alpha-adrenergic responses in isolated canine blood vessels.

Journal Article Chin Med J (Engl) · June 1993 The present study was designed to examine the effect of higher concentration of anisodamine on alpha-adrenergic responses in isolated canine blood vessels. Up to 10(-3) mol/L, anisodamine did not significantly affect the responses of saphenous vein to alph ... Link to item Cite